Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production

Methods in Molecular Biology

Volumn 1264, Issue , 2015, Pages 245-261

  Krumschnabel, Gerhard ^a   Fontana Ayoub, Mona ^a   Sumbalova, Zuzana ^a   Heidler, Juliana ^a   Gauper, Kathrin ^a   Fasching, Mario ^a   Gnaiger, Erich ^a  

^a NONE

Author keywords

Amplex Red; High resolution respirometry; Mouse brain mitochondria; Oxygraph; Resorufin; Respiration media; Substrate uncouple inhibitor titration

Indexed keywords

HYDROGEN PEROXIDE; RESORUFIN; REACTIVE OXYGEN METABOLITE;

AMPLEX RED ASSAY; ANIMAL CELL; ARTICLE; ASSAY; BIOENERGY; BRAIN MITOCHONDRION; FLUORESCENCE; MITOCHONDRIAL RESPIRATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; RESPIROMETRY; SENSITIVITY ANALYSIS; SUBSTRATE UNCOUPLER AND INHIBITOR TITRATION; TITRIMETRY; ANIMAL; BRAIN; CELL RESPIRATION; CHEMISTRY; DEVICES; DRUG EFFECTS; FLUOROMETRY; METABOLISM; MITOCHONDRION; OXYGEN CONSUMPTION; PROCEDURES;

ANIMALS; BRAIN; CELL RESPIRATION; FLUOROMETRY; HYDROGEN PEROXIDE; MICE; MITOCHONDRIA; OXYGEN CONSUMPTION; REACTIVE OXYGEN SPECIES;

EID: 84958674756     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2257-4_22     Document Type: Article

References (26)

1. Tahara EB, Navarete FD, Kowaltowski AJ (2009) Tissue-, substrate-, and site-specific characteristics of mitochondrial reactive oxygen species generation. Free Radic Biol Med 46: 1283–1297
2. Kowaltowski AJ, de Souza-Pinto NC, Castilho RF, Vercesi AE (2009) Mitochondria and reactive oxygen species. Free Radic Biol Med 47: 333–343
3. Dröse S, Brandt U (2012) Molecular mechanisms of superoxide production by the mitochondrial respiratory chain. Adv Exp Med Biol 748:145–169
4. Mohanty JG, Jaffe JS, Schulman ES, Raible DG (1997) A highly sensitive fl uorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative. J Immunol Methods 202:133–141
5. Zhou M, Diwu Z, Panchuk-Voloschina N et al (1997) A stable non fluorescent derivative of reso-rufi n for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidize and other oxidizes. Anal Biochem 253(2):162–168
6. Towne V, Will M, Oswald B, Zhao Q (2004) Complexities in horseradish peroxidase-catalyzed oxidation of dihydroxyphenoxazine derivatives: appropriate ranges for pH values and hydrogen peroxide concentrations in quantitative analysis. Anal Biochem 334:290–296
7. Summers FA, Zhao B, Ganini D et al (2013) Photo oxidation of Ample Red to resourcing: implications of exposing the Ample Red assay to light. Methods Enzymol 526:1–17
8. Starkov AA (2010) Measurement of mitochondrial ROS production. Methods Mol Biol 648:245–255
9. Malinska D, Kudin AP, Debska-Vielhaber G et al (2009) Quantification of superoxide production by mouse brain and skeletal muscle mitochondria. Methods Enzymol 456:419–437
10. Votyakova TV, Reynolds IJ (2004) Detection of hydrogen peroxide with Ample Red: interference by NADH and reduced glutathione auto-oxidation. Arch Biochem Biophys 431(1): 138–144
11. Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibers of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91–100
12. Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277–297
13. Pesta D, Gnaiger E (2012) High-resolution res-pirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25–58
14. Chinopoulos C, Vajda S, Csanady L, Mandi M, Mathe K, Adam-Vizi V (2009) A novel kinetic assay of mitochondrial ATP-ADP exchange rate mediated by the ANT. Biophys J 96:2490–2504
15. Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163–181
16. Fasching M, Gradl P, Gnaiger E (2014) O2k-fl uorescence LED2-module. Mitochondr Physiol Network 17.05(05):1–7. http://www.bioblast.at/index.php/MiPNet17.05
17. Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Klingenspor M, Heldmaier G (eds) Life in the cold. Springer, Heiderlberg, pp 431–442
18. Komary Z, Tretter L, Adam-Vizi V (2010) Membrane potential-related effect of calcium on reactive oxygen species generation in isolated brain mitochondria. Biochim Biophys Acta 1797:922–928
19. Fontana-Ayoub M, Fasching M, Gnaiger E (2014) Selected media and chemicals for respi-rometry with mitochondrial preparations. Mitochondr Physiol Network 03.02(17):1–9. http://bioblast.at/index.php/MiPNet03.02
20. Sumbalová Z, Kucharská J, Kristek F (2010) Losartan improved respiratory function and coenzyme Q content in brain mitochondria of young spontaneously hypertensive rats. Cell Mol Neurobiol 30:751–758
21. Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. Mitochondria physiology network 17.18. OROBOROS MiPNet Publications, Innsbruck, p 64
22. Frieden E, Walter C (1963) Prevalence and significance of the product inhibition of enzymes. Nature 198:834–837
23. Piwonski HM, Goomanovsk M, Bensimon D et al (2012) Allosteric inhibition of individual enzyme molecules trapped in lipid vesicles. Proc Natl Acad Sci U S A 109(22): E1437–E1443
24. Cooper WJ, Zika RG, Petasne RG, Plane JM (1988) photochemical formation of hydrogen peroxide in natural waters exposed to sunlight. Environ Sci Technol 22(10):1156–1160
25. Boveris A, Chance B (1973) the mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen. Biochem J 134:707–716
26. Tretter L, Adam-Vizi V (2012) High Ca 2+ load promotes hydrogen peroxide generation via activation of α-glycerophosphate dehydrogenate in brain mitochondria. Free Radic Biol Med 53:2119–21130