High-throughput real-time analysis of cell oxygenation using intracellular oxygen-sensitive probes

Methods in Molecular Biology

Volumn 1264, Issue , 2015, Pages 203-217

  Hynes, James ^a   Carey, Conn ^a  

^a NONE

Author keywords

Cell metabolism; Electron transport chain; High throughput; Hypoxia; Intracellular oxygen; Mitochondrial function; Oxidative phosphorylation; Oxygen sensitive probe; Oxygenation; Time resolved fluorescence

Indexed keywords

OXYGEN;

ADHERENT CELL; ARTICLE; CELL DENSITY; DEOXYGENATION; GLYCOLYSIS; HIGH THROUGHPUT SCREENING; INCUBATION TIME; INTRACELLULAR SPACE; MICROPLATE READER; MICROTITER PLATE ASSAY; MOLECULAR PROBE; MONOLAYER CULTURE; OXYGEN CONCENTRATION; OXYGEN SENSITIVE PROBE; OXYGENATION; PRIORITY JOURNAL; TEMPERATURE MEASUREMENT; CELL RESPIRATION; CULTURE TECHNIQUE; DEVICES; DRUG EFFECTS; HEPG2 CELL LINE; HUMAN; KINETICS; METABOLISM; MITOCHONDRION; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION; PROCEDURES;

CELL CULTURE TECHNIQUES; CELL RESPIRATION; GLYCOLYSIS; HEP G2 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INTRACELLULAR SPACE; KINETICS; MITOCHONDRIA; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION;

EID: 84958605395     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2257-4_19     Document Type: Article

References (14)

1. Michael RD, Gyorgy S (2010) Roles of mitochondria in human disease. Essays Biochem 47:115–137
2. Gottlieb R, Quarato G, Abel ED (2012) Mitochondria in cardiac disease. In: Patterson C, Willis MS (eds) Translational cardiology, Molecular and translational medicine. Humana, Totowa, NJ, pp 63–82
3. Lin MT, Beal MF (2006) Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature 443(7113):787–795
4. Lenihan CR, Taylor CT (2013) The impact of hypoxia on cell death pathways. Biochem Soc Trans 41(2):657–663
5. Taylor CT, Moncada S (2010) Nitric oxide, cytochrome C oxidase, and the cellular response to hypoxia. Arterioscler Thromb Vasc Biol 30(4):643–647
6. Sung HJ et al (2010) Mitochondrial respiration protects against oxygen-associated DNA damage. Nat Commun 1:5
7. Toussaint O, Weemaels G, Debacq-Chainiaux F, Scharffetter-Kochanek K, Wlaschek M (2011) Artefactual effects of oxygen on cell culture models of cellular senescence and stem cell biology. J Cell Physiol 226(2):315–321
8. Hynes J, Floyd S, Soini AE, O’Connor R, Papkovsky DB (2003) Fluorescence-based cell viability screening assays using water-soluble oxygen probes. J Biomol Screen 8(3):264–272
9. O’Riordan TC et al (2007) Sensing intracellular oxygen using near-infrared phosphorescent probes and live-cell fluorescence imaging. Am J Physiol Regul Integr Comp Physiol 292:R1613–R1620
10. Valeur B (2002) Molecular fluorescence: principles and applications. Wiley-VCH, New York, NY
11. Hynes J et al (2009) In vitro analysis of cell metabolism using a long-decay pH-sensitive lanthanide probe and extracellular acidification assay. Anal Biochem 390(1):21–28
12. Fercher A, Borisov SM, Zhdanov AV, Klimant I, Papkovsky DB (2011) Intracellular O2 sensing probe based on cell-penetrating phosphore scent nanoparticles. ACS Nano 5(7):5499–5508
13. Zhdanov AV et al (2010) Extracellular calcium depletion transiently elevates oxygen consumption in neurosecretory PC12 cells through activation of mitochondrial Na+/Ca2+ exchange. Biochim Biophys Acta Bioenerg 1797(9):1627–1637
14. Hynes J, Natoli E, Will Y (2009) Fluorescent pH and oxygen probes of the assessment of mitochondrial toxicity in isolated mitochondria and whole cells. In: Maines M, Costa LG, Hodson E, Reed JC (eds) Current protocols in toxicology. Wiley, New York, NY, pp 40:42.16.41–42.16.22