In vitro analysis of cell metabolism using a long-decay pH-sensitive lanthanide probe and extracellular acidification assay

Analytical Biochemistry

Volumn 390, Issue 1, 2009, Pages 21-28

  Hynes, James ^a   O'Riordan, Tomás C ^a   Zhdanov, Alexander V ^a   Uray, Georg ^b   Will, Yvonne ^c   Papkovsky, Dmitri B ^a  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b UNIVERSITY OF GRAZ   (Austria)

^c PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

Fluorescent pH sensitive lanthanide chelate; Glycolytic flux; Lifetime based pH sensing; Microplate assay; Mitochondrial dysfunction; Screening; Time resolved fluorescence

Indexed keywords

ACIDIFICATION; BUDGET CONTROL; CELL CULTURE; CELLS; DIAGNOSIS; FLUORESCENCE; PH SENSORS; PROBES; RARE EARTH ELEMENTS; SCREENING;

GLYCOLYTIC FLUX; LANTHANIDE CHELATES; MICROPLATE ASSAY; MITOCHONDRIAL DYSFUNCTION; PH SENSING; TIME-RESOLVED FLUORESCENCE;

METABOLISM;

ADENOSINE TRIPHOSPHATE; LANTHANIDE;

ACIDIFICATION; ARTICLE; CELL CULTURE; CELL METABOLISM; FLUORESCENCE; GLYCOLYSIS; IN VITRO STUDY; OXYGEN CONSUMPTION; PH; PRIORITY JOURNAL; SCREENING;

EID: 65649105140     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.04.016     Document Type: Article

References (36)

1. Amacher D.E. Drug-associated mitochondrial toxicity and its detection. Curr. Med. Chem. 12 (2005) 1829-1839
2. Lin M.T., and Beal M.F. Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature 443 (2006) 787-795
3. Lowell B.B., and Shulman G.I. Mitochondrial dysfunction and type 2 diabetes. Science 307 (2005) 384-387
4. Schapira A.H. Mitochondrial dysfunction in Parkinson's disease. Cell Death Differ. 14 (2007) 1261-1266
5. Hynes J., Floyd S., Soini A.E., O'Connor R., and Papkovsky D.B. Fluorescence-based cell viability screening assays using water-soluble oxygen probes. J. Biomol. Screen. 8 (2003) 264-272
6. Arain S., Weiss S., Heinzle E., John G.T., Krause C., and Klimant I. Gas sensing in microplates with optodes: influence of oxygen exchange between sample, air, and plate material. Biotechnol. Bioeng. 90 (2005) 271-280
7. O'Riordan T.C., Buckley D., Ogurtsov V., O'Connor R., and Papkovsky D.B. A cell viability assay based on monitoring respiration by optical oxygen sensing. Anal. Biochem. 278 (2000) 221-227
8. O'Riordan T.C., Fitzgerald K., Ponomarev G.V., Mackrill J., Hynes J., Taylor C., and Papkovsky D.B. Sensing intracellular oxygen using near-infrared phosphorescent probes and live-cell fluorescence imaging. Am. J. Physiol. Regul. Integr. Comp. Physiol. 292 (2007) R1613-R1620
9. Hynes J., Marroquin L.D., Ogurtsov V.I., Christiansen K.N., Stevens G.J., Papkovsky D.B., and Will Y. Investigation of drug-induced mitochondrial toxicity using fluorescence-based oxygen-sensitive probes. Toxicol. Sci. 92 (2006) 186-200
10. Poole R.C., and Halestrap A.P. Transport of lactate and other monocarboxylates across mammalian plasma membranes. Am. J. Physiol. Cell Physiol. 264 (1993) C761-C782
11. Deuticke B. Monocarboxylate transport in red blood cells: kinetics and chemical modification. Methods Enzymol. 173 (1989) 300-329
12. Boron W.F. Regulation of intracellular pH. Adv. Physiol. Educ. 28 (2004) 160-179
13. Hafeman D.G., Parce J.W., and McConnell H.M. Light-addressable potentiometric sensor for biochemical systems. Science 240 (1988) 1182-1185
14. Baumann W.H., Lehmann M., Schwinde A., Ehret R., Brischwein M., and Wolf B. Microelectronic sensor system for microphysiological application on living cells. Sens. Actuat. B 55 (1999) 77-89
15. Wolfbeis O.S., Fürlinger E., Kroneis H., and Marsoner H. Fluorimetric analysis: 1A study on fluorescent indicators for measuring near neutral ("physiological") pH values. Fresenius Z. Anal. Chem. 314 (1983) 119-124
16. Xu Z., Rollins A., Alcala R., and Marchant R.E. A novel fibre-optic pH sensor incorporating carboxy SNAFL-2 and fluorescent wavelength-ratiometric detection. J. Biomed. Mater. Res. 39 (1998) 9-15
17. Saari L.A., and Seitz W.R. PH sensor based on immobilized fluorescein amine. Anal. Chem. 54 (1982) 821-823
18. Malins C., Glever H.G., Keyes T.E., Vos J.G., Dressickc W.J., and MacCraith B.D. Sol-gel immobilised ruthenium(II) polypyridyl complexes as chemical transducers for optical pH sensing. Sens. Actuat. B 67 (2000) 89-95
19. Lobnik A., Majcena N., Niederreiterb K., and Uray G. Optical pH sensor based on the absorption of antenna generated europium luminescence by bromothymol blue in a sol-gel membrane. Sens. Actuat. B 74 (2001) 200-206
20. Kermis H.R., Kostov Y., Harms P., and Rao G. Dual excitation ratiometric fluorescent pH sensor for noninvasive bioprocess monitoring: development and application. Biotechnol. Prog. 18 (2002) 1047-1053
21. Lin H.J., Szmacinski H., and Lakowicz J.R. Lifetime-based pH sensors: indicators for acidic environments. Anal. Biochem. 269 (1999) 162-167
22. Kosch U., Klimant I., and Wolfbeis O.S. Long-lifetime based pH microoptodes without oxygen interferences. Fresenius Z. Anal. Chem. 364 (1999) 48-53
23. Draxler S., and Lippitsch M.E. PH sensors using fluorescence decay time. Sens. Actuat. B 29 (1995) 199-203
24. G. Uray, K. Niederreiter, Luminescent 4-trifluoromethyl-2-quinolones with long-wave UV absorption and the use thereof, US patent 6,635 (2003) 759.
25. 2+ sensors with increased dynamic range. BMC Biotechnol. 7 (2007) 37
26. Thedinga E., Kob A., Holst H., Keuer A., Drechsler S., Niendorf R., Baumann W., Freund I., Lehmann M., and Ehret R. Online monitoring of cell metabolism for studying pharmacodynamic effects. Toxicol. Appl. Pharmacol. 220 (2007) 33-44
27. Wu M., Neilson A., Swift A.L., Moran R., Tamagnine J., Parslow D., Armistead S., Lemire K., Orrell J., Teich J., Chomicz S., and Ferrick D.A. Multiparameter metabolic analysis reveals a close link between attenuated mitochondrial bioenergetic function and enhanced glycolysis dependency in human tumor cells. Am. J. Physiol. Cell Physiol. 292 (2007) C125-C136
28. Rodríguez-Enríquez S., Juárez O., Rodríguez-Zavala J.S., and Moreno-Sánchez R. Multisite control of the crabtree effect in ascites hepatoma cells. Eur. J. Biochem. 268 (2001) 2512-2519
29. Marroquin L.D., Hynes J., Dykens J.A., Jamieson J.D., and Will Y. Circumventing the Crabtree effect: replacing media glucose with galactose increases susceptibility of HepG2 cells to mitochondrial toxicants. Toxicol. Sci. 97 (2007) 539-547
30. Hafner F. Cytosensor microphysiometer'echnology and recent applications. Biosens. Bioelectron. 15 (2000) 149-158
31. Ross R.A., Spengler B.A., and Biedler J.L. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71 (1983) 741-747
32. McCormack J.G., Halestrap A.P., and Denton R.M. Role of calcium ions in regulation of mammalian intramitochondrial metabolism. Physiol. Rev. 70 (1990) 391-425
33. Kadenbach B. Intrinsic and extrinsic uncoupling of oxidative phosphorylation. Biochim. Biophys. Acta 1604 (2003) 77-94
34. Hansford R.G., and Zorov D. Role of mitochondrial calcium transport in the control of substrate oxidation. Mol. Cell. Biochem. 184 (1998) 359-369
35. 2+ exchanger and protein kinase C. J. Cell Biol. 163 (2003) 511-523
36. 2+ exchanger increases mitochondrial metabolism and potentiates glucose-stimulated insulin secretion in rat pancreatic islets. Diabetes 52 (2003) 965-973