Sucrose induced osmotic stress and photoperiod regimes enhanced the biomass and production of antioxidant secondary metabolites in shake-flask suspension cultures of Prunella vulgaris L.

Plant Cell, Tissue and Organ Culture

Volumn 124, Issue 3, 2016, Pages 573-581

  Fazal, Hina ^a,b   Abbasi, Bilal Haider ^a   Ahmad, Nisar ^c   Ali, Mohammad ^a   Ali, Shahid ^c  

^a QUAID I AZAM UNIVERSITY   (Pakistan)

^b PAKISTAN COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH   (Pakistan)

^c University of Swat   (Pakistan)

Author keywords

Flavonoids; pH; Prunella; Stress; Sucrose

Indexed keywords

ANTIOXIDANTS; BIOMASS; BIOMOLECULES; CELL CULTURE; FLAVONOIDS; METABOLITES; NAPHTHALENE; PH; PROTEINS; STRESSES; SUGAR (SUCROSE);

ANTI-OXIDANT ACTIVITIES; BIOACTIVE COMPOUNDS; CELL SUSPENSION CULTURES; DIFFERENTIAL EFFECT; MURASHIGE AND SKOOG MEDIUMS; NAPHTHALENEACETIC ACID; PRUNELLA; SECONDARY METABOLITES;

PLANTS (BOTANY);

EID: 84957431506     PISSN: 01676857     EISSN: 15735044     Source Type: Journal    
DOI: 10.1007/s11240-015-0915-z     Document Type: Article

References (42)

1. Ali M, Abbasi BH (2014) Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. J Photochem Photobiol B: Biol 140:223–227
2. Ali MB, Khatun S, Hahn EJ, Paek KY (2006) Enhancement of phenylpropanoid enzymes and lignin in Phalaenopsis orchid and their influence on plant acclimatization at different levels of photosynthetic photon flux. Plant Growth Regul 49:137–146
3. Baque MA, Elgirban A, Lee EJ, Paek KY (2012) Sucrose regulated enhanced induction of anthraquinone, phenolics, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.). Acta Physiol Plant 34:405–415
4. Barz W, Daniel S, Hinderer W, Jaques U, Kessmann H, Koster J, Tiemann K (1988) Elicitation and metabolism of phytoalexins in plant cell cultures. In: Pais M, Mavituna F, Novais J (eds) Plant cell biotechnol. NATO ASI Series. Springer, Berlin, pp 211–230
5. Bhakuni RS, Jain DC, Sharma RP, Kumar S (2001) Secondary metabolites of Artemisia annua and their biological activity. Curr Sci 80:35–48
6. Bidchol AM, Wilfred A, Abhijna P, Harish R (2011) Free radical scavenging activity of aqueous and ethanolic extract of Brassica oleracea L. var. italica. Food Bioproc Tech 4:1137–1143
7. Bourgaud F, Gravot A, Milesi S, Gonteir E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851
8. Canadanovic-Brunet JM, Djilas SM, Cetkovic GS, Tumbas VT (2005) Free-radical scavenging activity of wormwood (Artemisia absinthium L.) extracts. J Sci Food Agric 85:265–272
9. Chen Y, Yu M, Zhu Z, Zhang L, Guo Q (2013) Optimisation of potassium chloride nutrition for proper growth, physiological development and bioactive component production in Prunella vulgaris L. PLoS ONE 8:1–7
10. Cheung HY, Zhang QF (2008) Enhanced analysis of triterpenes, flavonoids and phenolic compounds in Prunella vulgaris L. by capillary zone electrophoresis with the addition of running buffer modifiers. J Chromatogr A 1213:231–238
11. Cui XH, Murthy HM, Wu CH, Paek KY (2010) Sucrose induced osmotic stress affects biomass, metabolite, and antioxidant levels in root suspension cultures of Hypericum perforatum L. Plant Cell, Tissue Organ Cult 103:7–14
12. Eilert U (1987) Elicitation: methodology and aspects of application. In: Constabel F, Vasil I (eds) Cell culture and somatic cell genetics of plants, vol 4. Academic Press, San Diego, pp 153–196
13. Fazal H, Abbasi BH, Ahmad N (2014) Optimization of adventitious root culture for production of biomass and secondary metabolites in Prunella vulgaris L. Appl Biotechnol Biochem 174:2086–2095
14. Feng L, Au-yeung W, Xu YH, Wang SS, Quan Zhu Q, Xiang P (2011) Oleanolic acid from Prunella vulgaris L. induces SPC-A-1 cell line apoptosis via regulation of bax, bad and Bcl-2 expression. Asian Pac J Cancer Prev 12:403–408
15. Ferri M, Righetti L, Tassoni A (2011) Increasing sucrose concentrations promote phenylpropanoid biosynthesis in grapevine cell cultures. J Plant Physiol 168:189–195
16. Ghasemzadeh A, Jaafar HZE, Rahmat A, Wahab PEM, Halim MRA (2010) Effect of different light intensities on total phenolics and flavonoids synthesis and antioxidant activities in young ginger varieties (Zingiber officinale Roscoe). Int J Mol Sci 11:3885–3897
17. Giri L, Dhyani P, Rawat S, Bhatt ID, Nandi SK, Rawal RS, Pande V (2012) In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: a rare Himalayan medicinal orchid. Ind Crops Prod 39:1–6
18. Golembiovska OI, Tsurkan AA (2013) Anthocyanins profiling of Prunella vulgaris L. grown in Ukraine. Pharm Innov J 2:42–48
19. Hohe A, Reski R (2002) Optimization of a bioreactor culture of the moss Physcomitrella patens for mass production of proptoplasts. Plant Sci 0:1–6
20. Huang R, Zhao M, Yang X, Huang J, Yang Y, Chen B, Tan J, Huang J, Li Z, Lv Y, Ji G (2013) Effects of Prunella vulgaris on the mice immune function. PLoS ONE 8:1–8
21. Khan MA, Abbasi BH, Ahmed N, Ali H (2013) Effects of light regimes on in vitro seed germination and silymarin content in Silybum marianum. Ind Crops Prod 46:105–110
22. Kim HJ, Chen F, Xi W, Choi JH (2006) Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.). J Agric Food Chem 54:7263–7269
23. Lagrimini LM (1991) Wound-induced deposition of polyphenols in transgenic plants over expressing peroxidase. Plant Physiol 96:577–583
24. Liu GM, Jia XB, Wang HB, Feng L, Chen Y (2009) Review about current status of cancer prevention for the chemical composition or composition and function mechanism of Prunella vulgaris. J Chin Med Mater 3:1920–1926
25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
26. Meratan AA, Gaffari SM, Nikram V (2009) In vitro organogenesis and antioxidant enzymes activity in Acanthophyllum sordidum. Biol Plant 53:5–10
27. Morkunas I, Marczak L, Stachowiak J, Stobiecki M (2005) Sucrose-induced lupine defense against Fusarium oxysporum sucrose-stimulated accumulation of isoflavonoids as a defense response of lupine to Fusarium oxysporum. Plant Physiol Biochem 43:363–373
28. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
29. Nayyar H, Gupta D (2006) Differential sensitivity of C3 and C4 plants to water deficit stress: association with oxidative stress and antioxidants. Environ Exp Bot 58:106–113
30. Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153
31. Rasool R, Ganai BA, Akbar S, Kamili AN, Masood A (2010) Phytochemical screening of Prunella vulgaris L. an important medicinal plant of Kashmir. Pak J Pharm Sci 23(4):399–402
32. Riedel H, Cai Z, Kütük O, Smetanska I (2010) Obtaining phenolic acids from cell cultures of various Artemisia species. Afr J Biotechnol 9:8805–8809
33. Sakamoto A, Murata N (2002) The role of glycine betaine in the protection of plants from stress: clues from transgenic plants. Plant, Cell Environ 25:163–171
34. See KS, Bhatt A, Keng CL (2011) Effect of sucrose and methyl jasmonate on biomass and anthocyanins production in cell suspension culture of Melastoma malabathricum (Melastomaceae). Rev Biol Trop 59:597–606
35. Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S (2009) Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 22:102–106
36. Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY (2006) Effect of Light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochem 41:1176–1185
37. Tariq U, Ali M, Abbasi BH (2014) Morphogenic and biochemical variations under different spectral lights in callus cultures of Artemisia absinthium L. J Photochem Photobiol B: Biol 130:264–271
38. Wind J, Smeekens S, Hanson J (2010) Sucrose: metabolite and signaling molecule. Phytochemistry 71:1610–1614
39. Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199
40. Wu CH, Murthy HN, Hahn EJ, Paek KY (2007) Large-scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactor for the production of chichoric acid, chlorogenic acid and cafraric acid. Biotechnol Lett 29:1179–1182
41. Zhang YH, Zhong JJ, Yu JT (1996) Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: manipulation of medium sucrose. J Biotechnol 51:49–56
42. Zhong JJ, Seki T, Kinoshita S, Yoshida T (1991) Effect of light irradiation on anthocyanin production by suspended culture of Perilla frutescens. Biotech Bioeng 38:653–658