Combined mass quantitation and phenotyping of intact extracellular vesicles by a microarray platform

Analytica Chimica Acta

Volumn 902, Issue , 2016, Pages 160-167

  Gagni, Paola ^a   Cretich, Marina ^a   Benussi, Luisa ^b   Tonoli, Elisa ^b   Ciani, Miriam ^b   Ghidoni, Roberta ^b   Santini, Benedetta ^c   Galbiati, Elisabetta ^c   Prosperi, Davide ^c   Chiari, Marcella ^a  

^a CNR   (Italy)

^b IRCCS ISTITUTO CENTRO SAN GIOVANNI DI DIO FATEBENEFRATELLI   (Italy)

^c UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

Biomarkers; Exosomes; Extracellular vesicles; Fluorescence; Interferometry; Microarrays

Indexed keywords

BIOMARKERS; FLUORESCENCE; INTERFEROMETRY; MICROARRAYS;

EXOSOMES; EXTRACELLULAR; HIGH SENSITIVITY; INTERCELLULAR COMMUNICATIONS; INTERFEROMETRIC MEASUREMENT; LABEL FREE; PHENOTYPING; SANDWICH IMMUNOASSAY;

BIOASSAY;

ANTIBODY; SILICON; SILICON DIOXIDE; NANOPARTICLE;

ARTICLE; CONTROLLED STUDY; EXOSOME; FEASIBILITY STUDY; FLUORESCENCE ANALYSIS; HEK293 CELL LINE; HUMAN; HUMAN CELL; IMMUNOASSAY; INTERFEROMETRY; MICROARRAY ANALYSIS; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SANDWICH IMMUNOASSAY; SENSITIVITY ANALYSIS; SKIN FIBROBLAST; WESTERN BLOTTING; CHEMISTRY; FLUORESCENCE; MASS SPECTROMETRY;

EXTRACELLULAR VESICLES; FLUORESCENCE; HUMANS; MASS SPECTROMETRY; NANOPARTICLES;

EID: 84956880106     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2015.10.017     Document Type: Article

References (35)

1. Valadi H., Ekström K., Bossios A., Sjöstrand M., Lee J.J., Lötvall J.O. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 2007, 9:654-659. 10.1038/ncb1596.
2. Raposo G., Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J. Cell Biol. 2013, 200:373-383. 10.1083/jcb.201211138.
3. Tetta C., Ghigo E., Silengo L., Deregibus M.C., Camussi G. Extracellular vesicles as an emerging mechanism of cell-to-cell communication. Endocrine 2013, 44:11-19. 10.1007/s12020-012-9839-0.
4. Lachenal G., Pernet-Gallay K., Chivet M., Hemming F.J., Belly A., Bodon G., et al. Release of exosomes from differentiated neurons and its regulation by synaptic glutamatergic activity. Mol. Cell. Neurosci. 2011, 46:409-418. 10.1016/j.mcn.2010.11.004.
5. Hood J.L., San Roman S., Wickline S.A. Exosomes released by melanoma cells prepare sentinel lymph nodes for tumor metastasis. Cancer Res. 2011, 71:3792-3801. 10.1158/0008-5472.CAN-10-4455.
6. Yuana Y., Sturk A., Nieuwland R. Extracellular vesicles in physiological and pathological conditions. Blood Rev. 2013, 27:31-39. 10.1016/j.blre.2012.12.002.
7. Robbins P.D., Morelli A.E. Regulation of immune responses by extracellular vesicles. Nat. Rev. Immunol. 2014, 14:195-208. 10.1038/nri3622.
8. Yoshioka Y., Kosaka N., Konishi Y., Ohta H., Okamoto H., Sonoda H., et al. Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. Nat. Commun. 2014, 5:3591. 10.1038/ncomms4591.
9. Varga Z., Yuana Y., Grootemaat A.E., van der Pol E., Gollwitzer C., Krumrey M., et al. Towards traceable size determination of extracellular vesicles. J. Extracell. Vesicles 2014, 3:1-10. 10.3402/jev.v3.23298.
10. Momen-Heravi F., Balaj L., Alian S., Tigges J., Toxavidis V., Ericsson M., et al. Alternative methods for characterization of extracellular vesicles. Front. Physiol. 2012, 3 SEP. 10.3389/fphys.2012.00354.
11. Wubbolts R., Leckie R.S., Veenhuizen P.T.M., Schwarzmann G., Möbius W., Hoernschemeyer J., et al. Proteomic and biochemical analyses of human B cell-derived exosomes: potential implications for their function and multivesicular body formation. J. Biol. Chem. 2003, 278:10963-10972. 10.1074/jbc.M207550200.
12. Lässer C., Eldh M., Lötvall J. Isolation and characterization of RNA-containing exosomes. J. Vis. Exp. 2012, 10.3791/3037.
13. van der Vlist E.J., Nolte-'t Hoen E.N.M., Stoorvogel W., Arkesteijn G.J.A., Wauben M.H.M. Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry. Nat. Protoc. 2012, 7:1311-1326. 10.1038/nprot.2012.065.
14. Jørgensen M., Bæk R., Pedersen S., Søndergaard E.K.L., Kristensen S.R., Varming K. Extracellular vesicle (EV) array: microarray capturing of exosomes and other extracellular vesicles for multiplexed phenotyping. J. Extracell. Vesicles 2013, 2:1-9. 10.3402/jev.v2i0.20920.
15. Jørgensen M.M., Bæk R., Varming K. Potentials and capabilities of the extracellular vesicle (EV) array. J. Extracell. Vesicles 2015, 4. (accessed April 23, 2015). http://www.journalofextracellularvesicles.net/index.php/jev/article/view/26048/xml_7.
16. Cretich M., Monroe M.R., Reddington A., Zhang X., Daaboul G.G., Damin F., et al. Interferometric silicon biochips for label and label-free DNA and protein microarrays. Proteomics 2012, 12:2963-2977. 10.1002/pmic.201200202.
17. Cretich M., Reddington A., Monroe M., Bagnati M., Damin F., Sola L., et al. Silicon biochips for dual label-free and fluorescence detection: application to protein microarray development. Biosens. Bioelectron. 2011, 26:3938-3943. 10.1016/j.bios.2011.03.016.
18. Ozkumur E., Needham J.W., Bergstein D.A., Gonzalez R., Cabodi M., Gershoni J.M., et al. Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications. Proc. Natl. Acad. Sci. U. S. A. 2008, 105:7988-7992. 10.1073/pnas.0711421105.
19. Ozkumur E., Yalçin A., Cretich M., Lopez C.A., Bergstein D.A., Goldberg B.B., et al. Quantification of DNA and protein adsorption by optical phase shift. Biosens. Bioelectron. 2009, 25:167-172. 10.1016/j.bios.2009.06.033.
20. Cretich M., Di Carlo G., Longhi R., Gotti C., Spinella N., Coffa S., et al. High sensitivity protein assays on microarray silicon slides. Anal. Chem. 2009, 81:5197-5203. 10.1021/ac900658c.
21. Cretich M., Breda D., Damin F., Borghi M., Sola L., Unlu S.M., et al. Allergen microarrays on high-sensitivity silicon slides. Anal. Bioanal. Chem. 2010, 398:1723-1733. 10.1007/s00216-010-4077-x.
22. Cretich M., Bagnati M., Damin F., Sola L., Chiari M. Overcoming mass transport limitations to achieve femtomolar detection limits on silicon protein microarrays. Anal. Biochem. 2011, 418:164-166.
23. Monroe M.R., Reddington A.P., Collins A.D., Laboda C., Cretich M., Chiari M., et al. Multiplexed method to calibrate and quantitate fluorescence signal for allergen-specific IgE. Anal. Chem. 2011, 83:9485-9491. 10.1021/ac202212k.
24. Gagni P., Sola L., Cretich M., Chiari M. Development of a high-sensitivity immunoassay for amyloid-beta 1-42 using a silicon microarray platform. Biosens. Bioelectron. 2013, 47:490-495.
25. Benussi L., Ghidoni R., Steinhoff T., Alberici A., Villa A., Mazzoli F., et al. Alzheimer disease-associated cystatin C variant undergoes impaired secretion. Neurobiol. Dis. 2003, 13:15-21. 10.1016/S0969-9961(03)00012-3.
26. Théry C., Amigorena S., Raposo G., Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr. Protoc. Cell Biol. 2006, (Chapter 3), Unit 3.22. 10.1002/0471143030.cb0322s30.
27. Cretich M., Pirri G., Damin F., Solinas I., Chiari M. A new polymeric coating for protein microarrays. Anal. Biochem. 2004, 332:67-74.
28. Kanwar S.S., Dunlay C.J., Simeone D.M., Nagrath S. Microfluidic device (ExoChip) for on-chip isolation, quantification and characterization of circulating exosomes. Lab. Chip 2014, 14:1891-1900. 10.1039/c4lc00136b.
29. Record M., Subra C., Silvente-Poirot S., Poirot M. Exosomes as intercellular signalosomes and pharmacological effectors. Biochem. Pharmacol. 2011, 81:1171-1182. 10.1016/j.bcp.2011.02.011.
30. Van Deun J., Mestdagh P., Sormunen R., Cocquyt V., Vermaelen K., Vandesompele J., et al. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J. Extracell. Vesicles 2014, 3. (accessed 23.04.15). http://www.journalofextracellularvesicles.net/index.php/jev/article/view/24858/35788.
31. Dragovic R.A., Gardiner C., Brooks A.S., Tannetta D.S., Ferguson D.J.P., Hole P., et al. Sizing and phenotyping of cellular vesicles using nanoparticle tracking analysis. Nanomedicine Nanotechnol. Biol. Med. 2011, 7:780-788. 10.1016/j.nano.2011.04.003.
32. Bacarese-Hamilton T., Mezzasoma L., Ardizzoni a., Bistoni F., Crisanti a. Serodiagnosis of infectious diseases with antigen microarrays. J. Appl. Microbiol. 2004, 96:10-17. 10.1046/j.1365-2672.2003.02111.x.
33. Anderson T., Wulfkuhle J., Liotta L., Winslow R.L., Petricoin E. Improved reproducibility of reverse-phase protein microarrays using array microenvironment normalization. Proteomics 2009, 9:5562-5566. 10.1002/pmic.200900505.
34. Cretich M., Damin F., Chiari M. Protein microarray technology: how far off is routine diagnostics?. Analyst 2014, 139:528-542. 10.1039/c3an01619f.
35. Platt G.W., Damin F., Swann M.J., Metton I., Skorski G., Cretich M., et al. Allergen immobilisation and signal amplification by quantum dots for use in a biosensor assay of IgE in serum. Biosens. Bioelectron. 2014, 52:82-88. 10.1016/j.bios.2013.08.019.