Simple Method to Prepare Oligonucleotide-Conjugated Antibodies and Its Application in Multiplex Protein Detection in Single Cells

Bioconjugate Chemistry

Volumn 27, Issue 1, 2016, Pages 217-225

  Gong, Haibiao ^a   Holcomb, Ilona ^a   Ooi, Aik ^a   Wang, Xiaohui ^a   Majonis, Daniel ^a   Unger, Marc A ^a   Ramakrishnan, Ramesh ^a  

^a FLUIDIGM CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; BINDERS; CHEMICAL DETECTION; COST EFFECTIVENESS; DNA; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS;

CLICK REACTION; DNA-TEMPLATE; GENOMIC STUDIES; GENOMICS TOOLS; ITS APPLICATIONS; MULTIPLEX PROTEINS; PROTEIN DETECTION; PROTEIN TARGETS; SIMPLE METHOD; SINGLE CELLS;

ANTIBODIES;

ANTIBODY CONJUGATE; BSG PROTEIN; CASPASE 3; CCNB1 PROTEIN; CSTB PROTEIN; CYCLIN A2; DEATH RECEPTOR 4; DEATH RECEPTOR 5; EPITHELIAL CELL ADHESION MOLECULE; MUCIN 1; OLIGONUCLEOTIDE; PROTEIN; SCATTER FACTOR RECEPTOR; UNCLASSIFIED DRUG; VASCULOTROPIN A; ANTIBODY; RECOMBINANT PROTEIN;

ANTIGEN BINDING; ARTICLE; CONTROLLED STUDY; CYCLOADDITION; DNA SYNTHESIS; FAST PROTEIN LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN EXPRESSION; SINGLE CELL ANALYSIS; SOLUBILITY; WESTERN BLOTTING; CELL LINE; CHEMISTRY; CLICK CHEMISTRY; GENETICS; LIMIT OF DETECTION; METABOLISM; POLYMERASE CHAIN REACTION; PROCEDURES;

ANTIBODIES; CELL LINE; CLICK CHEMISTRY; CYCLOADDITION REACTION; HUMANS; LIMIT OF DETECTION; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; PROTEINS; RECOMBINANT PROTEINS; SINGLE-CELL ANALYSIS;

EID: 84956761145     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/acs.bioconjchem.5b00613     Document Type: Article

References (25)

1. Sano, T., Smith, C. L., and Cantor, C. R. (1992) Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates Science 258, 120-2 10.1126/science.1439758
2. Fredriksson, S., Gullberg, M., Jarvius, J., Olsson, C., Pietras, K., Gustafsdottir, S. M., Ostman, A., and Landegren, U. (2002) Protein detection using proximity-dependent DNA ligation assays Nat. Biotechnol. 20, 473-7 10.1038/nbt0502-473
3. Lundberg, M., Eriksson, A., Tran, B., Assarsson, E., and Fredriksson, S. (2011) Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood Nucleic Acids Res. 39, e102 10.1093/nar/gkr424
4. Soderberg, O., Gullberg, M., Jarvius, M., Ridderstrale, K., Leuchowius, K. J., Jarvius, J., Wester, K., Hydbring, P., Bahram, F., and Larsson, L. G. et al. 2006, Direct observation of individual endogenous protein complexes in situ by proximity ligation Nat. Methods 3, 995-1000 10.1038/nmeth947
5. Mocanu, M. M., Varadi, T., Szollosi, J., and Nagy, P. (2011) Comparative analysis of fluorescence resonance energy transfer (FRET) and proximity ligation assay (PLA) Proteomics 11, 2063-70 10.1002/pmic.201100028
6. Zeng, D., Zeglis, B. M., Lewis, J. S., and Anderson, C. J. (2013) The growing impact of bioorthogonal click chemistry on the development of radiopharmaceuticals J. Nucl. Med. 54, 829-32 10.2967/jnumed.112.115550
7. Manova, R. K., Pujari, S. P., Weijers, C. A., Zuilhof, H., and van Beek, T. A. (2012) Copper-free click biofunctionalization of silicon nitride surfaces via strain-promoted alkyne-azide cycloaddition reactions Langmuir 28, 8651-63 10.1021/la300921e
8. van Hest, J. C. and van Delft, F. L. (2011) Protein modification by strain-promoted alkyne-azide cycloaddition ChemBioChem 12, 1309-12 10.1002/cbic.201100206
9. Hughes, A. J., Spelke, D. P., Xu, Z., Kang, C. C., Schaffer, D. V., and Herr, A. E. (2014) Single-cell western blotting Nat. Methods 11, 749-55 10.1038/nmeth.2992
10. Rissin, D. M., Kan, C. W., Campbell, T. G., Howes, S. C., Fournier, D. R., Song, L., Piech, T., Patel, P. P., Chang, L., and Rivnak, A. J. et al. 2010, Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations Nat. Biotechnol. 28, 595-9 10.1038/nbt.1641
11. Uhlen, M., Fagerberg, L., Hallstrom, B. M., Lindskog, C., Oksvold, P., Mardinoglu, A., Sivertsson, A., Kampf, C., Sjostedt, E., and Asplund, A. et al. 2015, Proteomics. Tissue-based map of the human proteome Science 347, 1260419 10.1126/science.1260419
12. Ullal, A. V., Peterson, V., Agasti, S. S., Tuang, S., Juric, D., Castro, C. M., and Weissleder, R. (2014) Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates Sci. Transl. Med. 6, 219ra9 10.1126/scitranslmed.3007361
13. Hansen, M. C., Nederby, L., Henriksen, M. O., Hansen, M., and Nyvold, C. G. (2014) Sensitive ligand-based protein quantification using immuno-PCR: A critical review of single-probe and proximity ligation assays Biotechniques 56, 217-28
14. Zimmerman, E. S., Heibeck, T. H., Gill, A., Li, X., Murray, C. J., Madlansacay, M. R., Tran, C., Uter, N. T., Yin, G., and Rivers, P. J. et al. 2014, Production of site-specific antibody-drug conjugates using optimized non-natural amino acids in a cell-free expression system Bioconjugate Chem. 25, 351-61 10.1021/bc400490z
15. Gong, H., Kovar, J. L., Baker, B., Zhang, A., Cheung, L., Draney, D. R., Correa, I. R., Jr., Xu, M. Q., and Olive, D. M. (2012) Near-infrared fluorescence imaging of mammalian cells and xenograft tumors with SNAP-tag PLoS One 7, e34003 10.1371/journal.pone.0034003
16. Rahim, M. K., Kota, R., and Haun, J. B. (2015) Enhancing reactivity for bioorthogonal pretargeting by unmasking antibody-conjugated trans-cyclooctenes Bioconjugate Chem. 26, 352-60 10.1021/bc500605g
17. Peng, X., Chen, H., Draney, D. R., Volcheck, W., Schutz-Geschwender, A., and Olive, D. M. (2009) A nonfluorescent, broad-range quencher dye for Forster resonance energy transfer assays Anal. Biochem. 388, 220-8 10.1016/j.ab.2009.02.024
18. Schwanhausser, B., Busse, D., Li, N., Dittmar, G., Schuchhardt, J., Wolf, J., Chen, W., and Selbach, M. (2011) Global quantification of mammalian gene expression control Nature 473, 337-42 10.1038/nature10098
19. Willison, K. R. and Klug, D. R. (2013) Quantitative single cell and single molecule proteomics for clinical studies Curr. Opin. Biotechnol. 24, 745-51 10.1016/j.copbio.2013.06.001
20. Wu, M. and Singh, A. K. (2012) Single-cell protein analysis Curr. Opin. Biotechnol. 23, 83-8 10.1016/j.copbio.2011.11.023
21. Assarsson, E., Lundberg, M., Holmquist, G., Bjorkesten, J., Thorsen, S. B., Ekman, D., Eriksson, A., Rennel Dickens, E., Ohlsson, S., and Edfeldt, G. et al. 2014, Homogenous 96-plex PEA immunoassay exhibiting high sensitivity, specificity, and excellent scalability PLoS One 9, e95192 10.1371/journal.pone.0095192
22. Henglein, B., Chenivesse, X., Wang, J., Eick, D., and Brechot, C. (1994) Structure and cell cycle-regulated transcription of the human cyclin A gene Proc. Natl. Acad. Sci. U. S. A. 91, 5490-4 10.1073/pnas.91.12.5490
23. Jacobberger, J. W., Sramkoski, R. M., Wormsley, S. B., and Bolton, W. E. (1999) Estimation of kinetic cell-cycle-related gene expression in G1 and G2 phases from immunofluorescence flow cytometry data Cytometry 35, 284-9 10.1002/(SICI)1097-0320(19990301)35:3<284::AID-CYTO12>3.0.CO;2-K
24. Gong, H., Kovar, J. L., Cheung, L., Rosenthal, E. L., and Olive, D. M. (2014) A comparative study of affibody, panitumumab, and EGF for near-infrared fluorescence imaging of EGFR- and EGFRvIII-expressing tumors Cancer Biol. Ther. 15, 185-93 10.4161/cbt.26719
25. Pollen, A. A., Nowakowski, T. J., Shuga, J., Wang, X., Leyrat, A. A., Lui, J. H., Li, N., Szpankowski, L., Fowler, B., and Chen, P. et al. 2014, Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex Nat. Biotechnol. 32, 1053-8 10.1038/nbt.2967