Antimicrobial activity of bacteriophage endolysin produced in nicotiana benthamiana plants

Journal of Microbiology and Biotechnology

Volumn 26, Issue 1, 2015, Pages 160-170

  Kovalskaya, Natalia ^a   Foster Frey, Juli ^a   Donovan, David M ^a   Bauchan, Gary ^a   Hammond, Rosemarie W ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Endolysin; Nicotiana benthamiana; Potato proteinase inhibitor I; Potato virus X; Transient expression; Vacuolar targeting

Indexed keywords

BACTERIAL PROTEIN; CP933 ENDOLYSIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; ENDOLYSIN; PROTEINASE; VIRAL PROTEIN;

AMINO TERMINAL SEQUENCE; ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIOPHAGE; CELL VACUOLE; CLAVIBACTER MICHIGANENSIS; CONTROLLED STUDY; CP933 GENE; CYTOLYSIS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GROWTH INHIBITION; NICOTIANA BENTHAMIANA; NONHUMAN; POTATO VIRUS X; PROTEIN SYNTHESIS; PROTEIN TARGETING; ACTINOBACTERIA; BIOSYNTHESIS; DRUG EFFECTS; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; TOBACCO;

ACTINOBACTERIA; ANTI-BACTERIAL AGENTS; BACTERIOPHAGES; ENDOPEPTIDASES; ESCHERICHIA COLI; TOBACCO; VIRAL PROTEINS;

EID: 84955593562     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1505.05060     Document Type: Article

References (42)

1. Borisjuk NV, Borisjuk LG, Logendra S, Petersen F, Gleba Y, Raskin I. 1999. Production of recombinant proteins in plant root exudates. Nat. Biotechnol. 17: 466-469.
2. Borysowski J, Weber-Dabrowska B, Gorski A. 2006. Bacteriophage endolysins as a novel class of antibacterial agent. Exp. Biol. Med. 231: 366-377.
3. Chapman S, Kavanagh T, Baulcombe D. 1992. Potato virus X as a vector for gene expression in plants. Plant J. 2: 549-557.
4. Company N, Nadal A, La Paz J-L, Martínez S, Rasche S, Schillberg S, et al. 2014. The production of recombinant cationic α-helical antimicrobial peptides in plant cells induces the formation of protein bodies derived from the endoplasmic reticulum. Plant Biotechnol. J. 12: 81-92.
5. Daniell H, Lee SB, Panchal T, Wiebe PO. 2001. Expression of the native cholera B toxin subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J. Mol. Biol. 311: 1001-1009.
6. Donini M, Lico C, Baschieri S, Conti S, Magliani W, Polonelli L, Benvenuto E. 2005. Production of an engineered killer peptide in Nicotiana benthamiana by using a Potato virus X expression system. Appl. Environ. Microbiol. 71: 6360-6367.
7. Donovan DM. 2007. Bacteriophage and peptidoglycan degrading enzymes with antimicrobial applications. Recent Pat. Biotechnol. 1: 1-10.
8. Donovan DM, Becker SC, Dong S, Baker JR, Foster-Frey JA, Pritchard DG. 2009. Peptidoglycan hydrolase enzyme fusions are uniquely suited for treating multi-drug resistant pathogens. Biotech. Int. 21: 6-10.
9. Drake PM, Chargelegue DM, Vine ND, van Dolleweerd CJ, Obregon P, Ma JK. 2003. Rhizosecretion of a monoclonal antibody protein complex from transgenic tobacco roots. Plant Mol. Biol. 52: 233-241.
10. Düring K, Porsch P, Mahn A, Brinkmann O, Gieffers W. 1999. The non-enzymatic microbicidal activity of lysozymes. FEBS Lett. 449: 93-100.
11. Filice GA, Nyman JA, Lexau C. 2010. Excess costs and utilization associated with Staphylococcus aureus infection. Infect. Control Hosp. Epidemiol. 31: 365-367.
12. Fischetti VA. 2008. Bacteriophage lysins as effective antibacterials. Curr. Opin. Microbiol. 11: 393-400.
13. Fischetti VA. 2010. Bacteriophage endolysins: a novel antiinfective to control gram-positive pathogens. Int. J. Med. Microbiol. 300: 357-362.
14. Haddix AC, Teutsch SM, Corso PS. 2003. Prevention Effectiveness: A Guide to Decision Analysis and Economic Evaluation, pp. 345-357. 2nd Ed. Oxford University Press, New York.
15. Jackson MA, Nutt KA, Hassall R, Rae AL. 2010. Comparative efficiency of subcellular targeting signals for expression of a toxic protein in sugarcane. Funct. Plant Biol. 37: 785-793.
16. Jauh G-Y, Fischer AM, Grimes HD, Ryan CA, Rogers JC. 1998. δ-Tonoplast intrinsic protein defines unique plant vacuole functions. Proc. Natl. Acad. Sci. USA 95: 12995-12999.
17. Jauh G-Y, Phillips TE, Rogers JC. 1999. Tonoplast intrinsic protein isoforms as markers for vacuolar functions. Plant Cell 11: 1867-1882.
18. Jørgensen CS, Ryder LR, Steinø A, Højrup P, Hansen J, Beyer NH, et al. 2003. Dimerization and oligomerization of the chaperone calreticulin. Eur. J. Biochem. 270: 4140-4148.
19. Komarnytsky S, Borisjuk NV, Borisjuk LG, Alam MZ, Raskin I. 2000. Production of recombinant proteins in tobacco guttation fluid. Plant Physiol. 124: 927-933.
20. Kovalskaya N, Hammond RW. 2009. Expression and functional characterization of the plant antimicrobial snakin- 1 and defensin recombinant proteins. Protein Expr. Purif. 63: 12-17.
21. Kovalskaya N, Zhao Y, Hammond RW. 2011. Antibacterial and antifungal activity of a snakin-defensin hybrid protein expressed in tobacco and potato plants. Open Plant Sci. J. 5: 29-42.
22. Lacomme C, Chapman S. 2008. Use of Potato virus X (PVX)- based vectors for gene expression and virus-induced gene silencing (VIGS). Curr. Protoc. Microbiol. 8: 161.1.1-161.1.13.
23. Lico C, Chen Q, Santi L. 2008. Viral vectors for production of recombinant proteins in plants. J. Cell. Physiol. 216: 366-377.
24. Loeffler JM, Fischetti VA. 2003. Synergistic lethal effect of a combination of phage lytic enzymes with different activities on penicillin-sensitive and -resistant Streptococcus pneumoniae strains. Antimicrob. Agents Chemother. 47: 375-377.
25. Loeffler JM, Nelson D, Fischetti VA. 2001. Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase. Science 294: 2170-2172.
26. Murray C, Sutherland PW, Phung MM, Lester MT, Marshall RK, Christeller JT. 2002. Expression of biotin-binding proteins, avidin and streptavidin, in plant tissues using plant vacuolar targeting sequences. Transgenic Res. 11: 199-214.
27. Oliveira H, Melo LDR, Santos SB, Nóbrega FL, Ferreira EC, Cerca N, et al. 2013. Molecular aspects and comparative genomics of bacteriophage endolysins. J. Virol. 87: 4558-4570.
28. Orito Y, Morita M, Hori K, Unno H, Tanji Y. 2004. Bacillus amyloliquefaciens phage endolysin can enhance permeability of Pseudomonas aeruginosa outer membrane and induce cell lysis. Appl. Microbiol. Biotechnol. 65: 105-109.
29. Pang T, Fleming TC, Pogliano K, Young R. 2013. Visualization of pinholin lesions in vivo. Proc. Natl. Acad. Sci. USA 110: E2054-E2063.
30. Pang T, Savva CG, Fleming KG, Struck DK, Young R. 2009. Structure of the lethal phage pinhole. Proc. Natl. Acad. Sci. USA 106: 18966-18971.
31. Park T, Struck DK, Dankenbring CA, Young R. 2007. The pinholin of lambdoid phage 21: control of lysis by membrane depolarization. J. Bacteriol. 189: 9135-9139.
32. Perna NT, Plunkett GIII, Burland V, Mau B, Glasner JD, Rose DJ, et al. 2001. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409: 529-533.
33. Rashel M, Uchiyama J, Ujihara T, Uehara Y, Kuramoto S, Sugihara S, et al. 2007. Efficient elimination of multidrugresistant Staphylococcus aureus by cloned lysin derived from bacteriophage phi MR11. J. Infect. Dis. 196: 1237-1247.
34. Saitoh H, Kiba A, Nishihara M, Yamamura S, Suzuki K, Terauchi R. 2001. Production of antimicrobial defensin in Nicotiana benthamiana with a Potato virus X vector. Mol. Plant Microbe Interact. 14: 111-115.
35. São-José C, Parreira R, Vieira G, Santos MA. 2000. The Nterminal region of the Oenococcus oeni bacteriophage fOg44 lysin behaves as a bona fide signal peptide in Escherichia coli and as a cis-inhibitory element, preventing lytic activity on oenococcal cells. J. Bacteriol. 182: 5823-5831.
36. Schmelcher M, Donovan DM, Loessner MJ. 2012. Bacteriophage endolysins as novel antimicrobials. Future Microbiol. 7: 1147-1171.
37. Shen Y, Mitchell MS, Donovan DM, Nelson DC. 2012. Phage-based enzybiotics, pp. 217-239. In Hyman P, Abedon ST (eds.). Bacteriophages in Health and Disease. CAB International, Wallingford, UK.
38. Tan M, Hegde RS, Jiang X. 2004. The P domain of novovirus capsid protein forms dimer and binds to histoblood group antigen receptors. J. Virol. 78: 6233-6242.
39. Tregoning JS, Nixon P, Kuroda H, Svab Z, Clare S, Bowe F, et al. 2003. Expression of tetanus toxin fragment C in tobacco chloroplasts. Nucleic Acids Res. 31: 1174-1179.
40. Wang I-N, Deaton J, Young R. 2003. Sizing the holin lesion with an endolysin-beta-galactosidase fusion. J. Bacteriol. 185: 779-787.
41. Wang I-N, Smith DL, Young R. 2000. Holins: the protein clocks of bacteriophage infections. Annu. Rev. Microbiol. 54: 799-825.
42. Xu M, Struck DK, Deaton J, Wang I-N, Young RY. 2004. A signal-arrest release sequence mediates export and control of the phage P1 endolysin. Proc. Natl. Acad. Sci. USA 101: 6415-6420.