An evaluation of new and established methods to determine T-DNA copy number and homozygosity in transgenic plants

Plant Cell and Environment

Volumn 39, Issue 4, 2016, Pages 908-917

  Głowacka, Katarzyna ^a,b   Kromdijk, Johannes ^a   Leonelli, Lauriebeth ^c   Niyogi, Krishna K ^c,d   Clemente, Tom E ^e   Long, Stephen P ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b INSTITUTE OF PLANT GENETICS   (Poland)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^e UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

DdPCR; Digital droplet PCR; QPCR; Segregation analysis; Selectable marker; Southern blot; TAIL PCR; Transformation

Indexed keywords

BIOASSAY; BIOLOGICAL ANALYSIS; BIOMARKER; BIOTRANSFORMATION; DNA; EXPERIMENTAL STUDY; GENE EXPRESSION; LABORATORY METHOD; POLYMERASE CHAIN REACTION; TRANSGENIC PLANT;

NICOTIANA TABACUM;

BACTERIAL DNA; T-DNA;

CHROMOSOME SEGREGATION; CROSS BREEDING; GENE DOSAGE; GENE LOCUS; GENETICS; HOMOZYGOTE; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SOUTHERN BLOTTING; TOBACCO; TRANSGENIC PLANT;

BLOTTING, SOUTHERN; CHROMOSOME SEGREGATION; CROSSES, GENETIC; DNA, BACTERIAL; GENE DOSAGE; GENETIC LOCI; HOMOZYGOTE; PLANTS, GENETICALLY MODIFIED; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; TOBACCO;

EID: 84955511045     PISSN: 01407791     EISSN: 13653040     Source Type: Journal    
DOI: 10.1111/pce.12693     Document Type: Article

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