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Volumn 164, Issue 2, 2003, Pages 183-187

A rapid method for the analysis of zygosity in transgenic plants

Author keywords

Ct cycle threshold; Heterozygous; Homozygous; Real time PCR

Indexed keywords

BIOASSAY; DNA; GENES; GROWTH KINETICS;

EID: 0037327510     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(02)00381-3     Document Type: Article
Times cited : (41)

References (10)
  • 1
    • 0033165712 scopus 로고    scopus 로고
    • Overexpression of Arabidopsis hexokinase in tomato plants inhibits growth, reduces photosynthesis, and induces rapid senescence
    • N. Dai, A. Schaffer, M. Petreikov, Y. Shahak, Y. Giller, K. Ratner, A. Levine, D. Granot, Overexpression of Arabidopsis hexokinase in tomato plants inhibits growth, reduces photosynthesis, and induces rapid senescence, Plant Cell 11 (1999) 1253-1266.
    • (1999) Plant Cell , vol.11 , pp. 1253-1266
    • Dai, N.1    Schaffer, A.2    Petreikov, M.3    Shahak, Y.4    Giller, Y.5    Ratner, K.6    Levine, A.7    Granot, D.8
  • 2
    • 0242495513 scopus 로고    scopus 로고
    • Homogenous assay system using the nuclease activity of as nucleic acid polymerase. U.S. Patent no. 5 210 015 May 11 (1993)
    • D.H. Gelfand, P.M. Holland, R.K. Saiki, R.M. Watosno, Homogenous assay system using the nuclease activity of as nucleic acid polymerase. U.S. Patent no. 5 210 015 May 11 (1993).
    • Gelfand, D.H.1    Holland, P.M.2    Saiki, R.K.3    Watosno, R.M.4
  • 3
    • 0034953244 scopus 로고    scopus 로고
    • Quantitative real-time PCR assay for determining transgene copy number in transformed plants
    • D.J. Ingham, S. Beer, S. Money, G. Hansen, Quantitative real-time PCR assay for determining transgene copy number in transformed plants, Biotechniques 31 (2001) 132-140.
    • (2001) Biotechniques , vol.31 , pp. 132-140
    • Ingham, D.J.1    Beer, S.2    Money, S.3    Hansen, G.4
  • 4
    • 0028834086 scopus 로고
    • Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
    • K.J. Livak, S.J. Flood, J. Marmaro, W. Giusti, K. Deetz, Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization, PCR Methods Appl. 4 (1995) 357-362.
    • (1995) PCR Methods Appl. , vol.4 , pp. 357-362
    • Livak, K.J.1    Flood, S.J.2    Marmaro, J.3    Giusti, W.4    Deetz, K.5
  • 6
    • 0027550491 scopus 로고
    • Isolation and characterization of fruit vacuolar invertase genes from two tomato species and temporal differences in mRNA levels during fruit ripening
    • K.J. Elliot, W.O. Butler, C.D. Dikenson, Y. Konno, T.S. Vedvick, L. Fitzmaurice, T.E. Mirkov, Isolation and characterization of fruit vacuolar invertase genes from two tomato species and temporal differences in mRNA levels during fruit ripening, Plant Mol. Biol. 21 (1993) 515-524.
    • (1993) Plant Mol. Biol. , vol.21 , pp. 515-524
    • Elliot, K.J.1    Butler, W.O.2    Dikenson, C.D.3    Konno, Y.4    Vedvick, T.S.5    Fitzmaurice, L.6    Mirkov, T.E.7
  • 7
  • 8
    • 84982358134 scopus 로고
    • A revised medium for rapid growth and bioassay with tobacco tissue cultures
    • T. Murashige, F. Skoog, A revised medium for rapid growth and bioassay with tobacco tissue cultures, Plant Physiol. 15 (1962) 473-496.
    • (1962) Plant Physiol. , vol.15 , pp. 473-496
    • Murashige, T.1    Skoog, F.2
  • 9
    • 0025978277 scopus 로고
    • A simple and rapid method for the preparation of plant genomic DNA for PCR analysis
    • K. Edwards, C. Johnstone, C. Thompson, A simple and rapid method for the preparation of plant genomic DNA for PCR analysis, Nucleic Acids Res. 19 (1991) 1349.
    • (1991) Nucleic Acids Res. , vol.19 , pp. 1349
    • Edwards, K.1    Johnstone, C.2    Thompson, C.3
  • 10
    • 0036008336 scopus 로고    scopus 로고
    • Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR
    • L. Tesson, J.M. Heslan, S. Menoret, I. Anegon, Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR, Transgenic Res. 11 (2002) 43-48.
    • (2002) Transgenic Res. , vol.11 , pp. 43-48
    • Tesson, L.1    Heslan, J.M.2    Menoret, S.3    Anegon, I.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.