Fast-Tracking Determination of Homozygous Transgenic Lines and Transgene Stacking Using a Reliable Quantitative Real-Time PCR Assay

Applied Biochemistry and Biotechnology

Volumn 175, Issue 2, 2015, Pages 996-1006

  Wang, Xianghong ^a   Jiang, Daiming ^a   Yang, Daichang ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Molecular breeding; Quantitative real time PCR; Rice RBE4; Transgenic rice; Transgenic stacking

Indexed keywords

GENES; PLANTS (BOTANY);

MOLECULAR BREEDING; QUANTITATIVE REAL TIME PCR; RICE RBE4; TRANSGENIC RICE; TRANSGENICS;

POLYMERASE CHAIN REACTION;

1 DEOXY DEXTRO XYLULOSE 5 PHOSPHATE REDUCTOISOMERASE; 1,4 ALPHA GLUCAN BRANCHING ENZYME; ELONGATION FACTOR 1ALPHA; OS3BET3 PROTEIN; OS4TRS20 PROTEIN; PHOSPHATE; PROTEIN; RICE ACTIN PROTEIN; RICE ROOT SPECIFIC PROTEIN; STARCH; SUCROSE; SUCROSE PHOSPHATE SYNTHASE; SYNTHETASE; UNCLASSIFIED DRUG; GENETIC MARKER;

ARTICLE; COPY NUMBER VARIATION; GENE AMPLIFICATION; GENE INSERTION; GENETIC ANALYSIS; GENETIC SCREENING; GENETIC TRANSFORMATION; MOLECULAR BIOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT BREEDING; PLANT IDENTIFICATION; REAL TIME POLYMERASE CHAIN REACTION; RICE; SOUTHERN BLOTTING; TRANSGENE; TRANSGENIC PLANT; TRANSGENICS; BREEDING; GENE LOCUS; GENETIC MARKER; GENETICS; HOMOZYGOTE; PROCEDURES;

BREEDING; GENETIC LOCI; GENETIC MARKERS; HOMOZYGOTE; PLANTS, GENETICALLY MODIFIED; REAL-TIME POLYMERASE CHAIN REACTION; TRANSGENES;

EID: 84921051387     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-014-1322-3     Document Type: Article

References (19)

1. Baeumler, S., Wulff, D., Tagliani, L., & Song, P. (2006). Journal of Agricultural and Food Chemistry, 54, 6527–6534.
2. De Preter, K., Speleman, F., Combaret, V., Lunec, J., Laureys, G., Eussen, B. H., Francotte, N., Board, J., Pearson, A. D., De Paepe, A., Van Roy, N., & Vandesompele, J. (2002). Modern Pathology, 15, 159–166.
3. Higuchi, R., Fockler, C., Dollinger, G., & Watson, R. (1993). Biotechnology (NY), 11, 1026–1030.
4. Ahmed, F. E. (2002). Trends in Biotechnology, 20, 215–223.
5. Schmittgen, T. D., & Livak, K. J. (2008). Nature Protocols, 3, 1101–1108.
6. Heid, C. A., Stevens, J., Livak, K. J., & Williams, P. M. (1996). Genome Research, 6, 986–994.
7. Bubner, B., & Baldwin, I. T. (2004). Plant Cell Reports, 23, 263–271.
8. Yang, L., Ding, J., Zhang, C., Jia, J., Weng, H., Liu, W., & Zhang, D. (2005). Plant Cell Reports, 23, 759–763.
9. Yi, C. X., Zhang, J., Chan, K. M., Liu, X. K., & Hong, Y. (2008). Analytical Biochemistry, 375, 150–152.
10. Chen, S., Li, X., Liu, X., Xu, H., Meng, K., Xiao, G., Wei, X., Wang, F., & Zhu, Z. (2005). Plant Cell Reports, 23, 625–631.
11. Zhang, L., Shi, J., Jiang, D., Stupak, J., Ou, J., Qiu, Q., An, N., Li, J., & Yang, D. (2013). Journal of Biotechnology, 164, 300–308.
12. Sambrook, J., Russell, D.W. (2001). CSHL press.
13. Livak, K. J., & Schmittgen, T. D. (2001). Methods, 25, 402–408.
14. Hein, J., Schellenberg, U., Bein, G., & Hackstein, H. (2001). Scandinavian Journal of Immunology, 54, 285–291.
15. Zhang, Z., & Hu, J. (2007). Toxicological Sciences, 95, 356–368.
16. Mizuno, K., Kobayashi, E., Tachibana, M., Kawasaki, T., Fujimura, T., Funane, K., Kobayashi, M., & Baba, T. (2001). Plant and Cell Physiology, 42, 349–357.
17. Ji, W., Cai, L., Wright, M. B., Walker, G., Salgam, P., Vater, A., & Lindpaintner, K. (2000). Journal of Structural and Functional Genomics, 1, 1–7.
18. Bubner, B., Gase, K., & Baldwin, I. T. (2004). BMC Biotechnology, 4, 14.
19. Mieog, J. C., Howitt, C. A., & Ral, J. P. (2013). BMC Plant Biology, 13, 71.