Acoustic Methods to Monitor Protein Crystallization and to Detect Protein Crystals in Suspensions of Agarose and Lipidic Cubic Phase

Journal of Laboratory Automation

Volumn 21, Issue 1, 2016, Pages 107-114

  Ericson, Daniel L ^a,b   Yin, Xingyu ^a,c   Scalia, Alexander ^a,d   Samara, Yasmin N ^a,e   Stearns, Richard ^f   Vlahos, Harry ^f   Ellson, Richard ^f   Sweet, Robert M ^a   Soares, Alexei S ^a  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

^b UNIVERSITY AT BUFFALO   (United States)

^c STONY BROOK UNIVERSITY   (United States)

^d BINGHAMTON UNIVERSITY   (United States)

^e FEDERAL UNIVERSITY OF SANTA MARIA   (Brazil)

^f Labcyte Inc   (United States)

Author keywords

acoustic droplet ejection; crystal detection; crystallization; crystallography; lipidic cubic phase; sonar; visualization

Indexed keywords

CRYSTALLIZATION; CRYSTALLOGRAPHY; DROPS; FLOW VISUALIZATION; PROTEINS; SIGNAL DETECTION; SOLUTIONS; SONAR;

ACOUSTIC DROPLET EJECTIONS; ACOUSTIC REFLECTION; CHARACTERISTIC FREQUENCIES; CRYSTALLIZATION PLATES; LABORATORY TECHNIQUES; LIPIDIC CUBIC PHASE; PROTEIN CRYSTALLIZATION; SMALL PROTEIN CRYSTALS;

ACOUSTIC MEASURING INSTRUMENTS;

AGAROSE; LIPID; PROTEIN; SEPHAROSE; SUSPENSION;

ACOUSTIC DROPLET EJECTION; ACOUSTIC TECHNOLOGY; ACOUSTICS; ARTICLE; CHEMICAL PHENOMENA; CRYSTAL DETECTION; CRYSTAL REFLECTION; CRYSTALLIZATION; CRYSTALLOGRAPHY; DRUG DESIGN; ECHOLOCATION; LIPIDIC CUBIC PHASE; LIQUID HANDLING INSTRUMENT; LIQUID HANDLING TECHNOLOGY; PROTEIN CRYSTALLIZATION; CHEMISTRY; LABORATORY AUTOMATION; PROCEDURES; SUSPENSION;

ACOUSTICS; AUTOMATION, LABORATORY; CHEMISTRY; CRYSTALLIZATION; PROTEINS; SEPHAROSE; SUSPENSIONS;

EID: 84955454314     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1177/2211068215616365     Document Type: Article

References (16)

1. A.S.SoaresM.A.EngelR.Stearns. Acoustically Mounted Microcrystals Yield High-Resolution X-Ray Structures. Biochemistry2011, 50, 4399–4401.
2. C.G.RoesslerA.KuczewskiR.Stearns. Acoustic Methods for High-Throughput Protein Crystal Mounting at Next-Generation Macromolecular Crystallographic Beamlines. J. Synchrotron Rad. 2013, 20, 805–808.
3. C.M.CuttittaD.L.EricsonA.Scalia. Acoustic Transfer of Protein Crystals from Agarose Pedestals to Micromeshes for High-Throughput Screening. Acta. Cryst. 2015, D71, 94–103.
4. A.G.VillasenorA.WongA.Shao. Nanolitre-Scale Crystallization Using Acoustic Liquid-Transfer Technology. Acta. Cryst. 2012, D68, 893–900.
5. A.G.VillasenorA.WongA.Shao. Acoustic Matrix Microseeding: Improving Protein Crystal Growth with Minimal Chemical Bias. Acta. Cryst. 2010, D66, 568–576.
6. X.YinA.ScaliaL.Leroy. Hitting the Target: Fragment Screening with Acoustic In Situ Co-Crystallization of Proteins Plus Fragment Libraries on Pin-Mounted Data-Collection Micromeshes. Acta. Cryst. 2014, D70, 1177–1189.
7. R.D.WamplerD.J.KissickC.J.Dehen. Selective Detection of Protein Crystals by Second Harmonic Microscopy. J. Am. Chem. Soc. 2008, 130, 14076–14077.
8. M.NakazawaM.TabaruT.Aoyagi. Ultrasonics, Ferroelectrics, and Frequency Control. IEEE Transactions2013, 60, 2175–2188.
9. Square One Systems Design. UV Crystal Characterization Station. System Description Publication, 2014.
10. L.M.HaupertG.J.SimpsonScreening of Protein Crystallization Trials by Second Order Nonlinear Optical Imaging of Chiral Crystals (SONICC). Methods2011, 55, 379–386.
11. S.B.PothineniT.StrutzV.S.LamzinAutomated Detection and Centring of Cryocooled Protein Crystals. Acta. Cryst. 2006, D62, 1358–1368.
12. A.HérouxM.AllaireR.Buono. Macromolecular Crystallography Beamline X25 at the NSLS. J. Synchrotron. Rad. 2014, 21, 627–632.
13. B.HadimiogluR.StearnsR.Ellson. Moving Liquids with Sound: The Physics of Acoustic Droplet Ejection for Robust Laboratory Automation in Life Sciences. J. Lab. Autom., in press.
14. V.CherezovLipidic Cubic Phase Technologies for Membrane Protein Structural Studies. Curr. Opinion Struc. Bio. 2011, 21, 559–566.
15. H.S.GillEvaluating the Efficacy of Tryptophan Fluorescence and Absorbance as a Selection Tool for Identifying Protein Crystals. Acta. Cryst. 2010, F66, 364–372.
16. V.StojanoffJ.JakoncicD.A.Oren. From Screen to Structure with a Harvestable Microfluidic Device. Acta. Cryst. 2011, F67, 971–975.