Isolation of exosomes from the plasma of HIV-1 positive individuals

Journal of Visualized Experiments

Volumn 2016, Issue 107, 2016, Pages

  Konadu, Kateena Addae ^a   Huang, Ming Bo ^a   Roth, William ^a   Armstrong, Wendy ^b   Powell, Michael ^a   Villinger, Francois ^b,c   Bond, Vincent ^a  

^a MOREHOUSE SCHOOL OF MEDICINE   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c EMORY UNIVERSITY   (United States)

Author keywords

Cytokines; Exosomes; HIV 1; Immunology; Iodixanol; Issue 107; Plasma; Velocity Gradients

Indexed keywords

BIOLOGICAL MARKER; CYTOKINE; LEUKOCYTE ANTIGEN;

BLOOD; CELL CULTURE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXOSOME; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; METABOLISM; PROCEDURES; ULTRACENTRIFUGATION; VIRION; VIROLOGY;

ANTIGENS, CD; BIOMARKERS; CELLS, CULTURED; CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXOSOMES; HIV INFECTIONS; HIV-1; HUMANS; ULTRACENTRIFUGATION; VIRION;

EID: 84954560336     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/53495     Document Type: Article

References (27)

1. UNAIDS Report on the Global AIDS Epidemic. Joint United Nations Programme on HIV/AIDS (UNAIDS), Geneva Switzerland. (2013).
2. Levacher, M., Hulstaert, F., Tallet, S., Ullery, S., Pocidalo, J. J. Bach BA. The significance of activation markers on CD8 lymphocytes in human immunodeficiency syndrome: staging and prognostic value. Clin Exp Immun. 90, 376-382 (1992).
3. Giorgi, J. V., Liu, Z., Hultin, L. E., Cumberland, W. G., Hennessey, K., Detels, R. Elevated levels of CD38+ CD8+ T cells in HIV infection add to the prognostic value of low CD4+ T cell levels: results of 6 years of follow-up. The Los Angeles Center, Multicenter AIDS Cohort Study. J Acq Immun Def Synd. 6, 904-912 (1993).
4. Bofill, M., et al. Increased numbers of primed activated CD8+CD38+CD45RO+ T cells predict the decline of CD4+ T cells in HIV-1-infected patients. AIDS. 10, 827-834 (1996).
5. Liu, Z., Cumberland, W. G., Hultin, L. E., Prince, H. E., Detels, R., Giorgi, J. V. Elevated CD38 antigen expression on CD8+ T cells is a stronger marker for the risk of chronic HIV disease progression to AIDS and death in the Multicenter AIDS Cohort Study than CD4+ cell count, soluble immune activation markers, or combinations of HLA-DR and CD38 expression. J Acq Immun Def Synd.16, 83-92 (1997).
6. Douek, D. C., Roederer, M., Koup, R. A. Emerging concepts in the immunopathogenesis of AIDS. Annu Rev Med. 60, 471-484. (2009).
7. Roberts, L., et al. Plasma cytokine levels during acute HIV-1 infection predict HIV disease progression. AIDS. 24, 819-831. (2010).
8. Mueller, Y. M., et al. Interleukin-15 increases effector memory CD8+ T cells and NK Cells in simian immunodeficiency virus-infected macaques. J Virol. 79, 4877-4885. (2005).
9. Picker, L. J., et al. IL-15 induces CD4 effector memory T cell production and tissue emigration in nonhuman primates. J Clin Invest. 116, 1514-1524 (2006).
10. Mueller, Y. M., et al. IL-15 treatment during acute simian immunodeficiency virus (SIV) infection increases viral set point and accelerates disease progression despite the induction of stronger SIV-specific CD8+ T cell responses. J Immunol. 180, 350-360 (2008).
11. O'Brien, M., Manches O, Bhardwaj N, Plasmacytoid dendritic cells in HIV infection. Adv Exp Med Biol. 762, 71-107 (2013).
12. Chihara, T., et al. HIV-1 proteins preferentially activate anti-inflammatory M2-type macrophages. J Immunol. 188, 3620-3627 (2012).
13. Johnstone, R. M., Adam, M., Hammond, J. R., Orr, L., Turbide, C. Vesicle formation during reticulocyte maturation. J Bio Chem. 262, 9412-9420 (1987).
14. Meckes Jr., D. G., Raab-Traub, N. Microvesicles and viral infection. J. Virol. 85, 12844-12854 (2011).
15. Campbell, T.D, Khan M., Huang, MB., Bond, VC., Powell, MD. HIV-1 Nef protein is secreted into vesicles that can fuse with target cells and virions. Ethn. Dis. 18[2 Suppl 2], S2-S9 PMID: 18646314 (2008).
16. Muratori, C., et al. Massive Secretion by T Cells Is Caused by HIV Nef in Infected Cells and by Nef Transfer to Bystander Cells. Cell Host. Microbe. 6, 218-230. (2009).
17. Lenassi, M., et al. HIV Nef is Secreted in Exosomes and Triggers Apoptosis in Bystander CD4 T Cells. Traffic. 11, 110-122 (2009).
18. Raymond, A. D., et al. HIV Type 1 Nef Is Released from Infected Cells in CD45+ Microvesicles and Is Present in the Plasma of HIV-Infected Individuals. AIDS Res Hum Retrovir. 27, 167-178 /aid.2009.0170 (2011).
19. James, C. O., Huang, M-B., Khan, M., Garcia-Barrio, M., Powell, M. D., Bond, V. C. Extracellular Nef Protein Targets CD4+ T Cells for Apoptosis by Interacting with CXCR4 Surface Receptors. Journal of Virology. 78, 3099-3109 (2004).
20. Huang, M. B., Jin, L. L., James, C.O., Khan, M., Powell, M. D., Bond, V. C. Characterization of Nef-CXCR4 Interactions Important for Apoptosis Induction. Journal of Virology. 78,11084-11096 (2004).
21. Konadu, K. A., et al. Association of cytokines with exosomes in the plasma of HIV-1-seropositive individuals. Journal of Infectious Diseases. (2014).
22. Cantin, R. Diou, J. Belanger, D. Tremblay, AM. Gilbert, C. Discrimination between exosomes and HIV-1: purification of both vesicles from cellfree supernatants. J Immunol Methods. 338, 21-30 (2008).
23. Witwer, K. W., et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J Extracellular Vesicles. 2, 20360 (2013).
24. Lässer, C. Eldh, M., Lötvall, J. Isolation and Characterization of RNA-Containing Exosomes. J. Vis. Exp. (59), e3037 (2012).
25. McDonald, M. K., Capasso, K. E., Ajit, S. K. Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media. J. Vis. Exp. (76), e50294 (2013).
26. Kanchi, R. R., Khosroheidari, M., DiStefano, J. K. A Modified Precipitation Method to Isolate Urinary Exosomes. J. Vis. Exp. (95), e51158 (2015).
27. Jan Lötvall, J., et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. J Extracell Vesicles. 3:26913 (2014).