Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography

Journal of Chromatography A

Volumn 1429, Issue , 2016, Pages 292-303

  Cunha, Bárbara ^a,b   Silva, Ricardo J S ^a,b   Aguiar, Tiago ^a,b   Serra, Margarida ^a,b   Daicic, John ^c   Maloisel, Jean Luc ^c   Clachan, John ^c   Åkerblom, Anna ^c   Carrondo, Manuel J T ^b,d   Peixoto, Cristina ^a,b   Alves, Paula M ^a,b  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

^c GE Healthcare Bio Sciences AB   (Sweden)

^d UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

Cell purification; Cell washing; Downstream processing; Expanded bed chromatography; Human mesenchymal stem cells

Indexed keywords

CELL CULTURE; CELLS; CHROMATOGRAPHY; CYTOLOGY; ECONOMIC AND SOCIAL EFFECTS; PACKED BEDS; PROTEINS; PURIFICATION; RECOVERY; STEM CELLS; WASHING;

CELL PURIFICATION; CLINICAL APPLICATION; DOWNSTREAM-PROCESSING; EXPANDED BED ADSORPTION; EXPANDED BEDS; HUMAN MESENCHYMAL STEM CELLS; PACKED BED CHROMATOGRAPHY; TANGENTIAL FLOW FILTRATIONS (TFF);

MOLECULAR BIOLOGY;

ADSORPTION KINETICS; ARTICLE; CELL ADHESION; CELL DIFFERENTIATION; CELL EXPANSION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; EXPANDED BED CHROMATOGRAPHY; FILTRATION; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; MESENCHYMAL STEM CELL; PRIORITY JOURNAL; PROTEIN METABOLISM; TANGENTIAL FLOW FILTRATION; CELL SURVIVAL; CHROMATOGRAPHY; CYTOLOGY; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMA CELL; PROCEDURES;

CELL SURVIVAL; CHROMATOGRAPHY; FILTRATION; HUMANS; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMAL CELLS;

EID: 84954198315     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2015.12.052     Document Type: Article

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