Optogenetics for in vivo cardiac pacing and resynchronization therapies

Nature Biotechnology

Volumn 33, Issue 7, 2015, Pages 750-754

  Nussinovitch, Udi ^a,b   Gepstein, Lior ^a,b  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^b Rambam Health Care Campus ^*  (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

HEART; VIRUSES;

ACTIVATION TIME; ADENO-ASSOCIATED VIRUS; CARDIAC CONDUCTIONS; DIFFUSE ILLUMINATION; ELECTRICAL SYNCHRONIZATION; OPTICAL MAPPING; RESYNCHRONIZATION; RESYNCHRONIZATION THERAPY;

CARDIAC RESYNCHRONIZATION THERAPY;

PARVOVIRUS VECTOR; RHODOPSIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLUE LIGHT; CARDIAC RESYNCHRONIZATION THERAPY; CARDIOVASCULAR GENE THERAPY; CHANNELRHODOPSIN 2 GENE; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; HEART PACING; HEART VENTRICLE CONTRACTION; HUMAN; HUMAN CELL; ILLUMINATION; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; ISOLATED HEART; MALE; NERVE CELL EXCITABILITY; NONHUMAN; OPTOGENETICS; PRIORITY JOURNAL; RAT; TRANSGENE; VIRAL GENE DELIVERY SYSTEM; ANIMAL; DEPENDOPARVOVIRUS; FLUORESCENCE IMAGING; GENETICS; HEART; IMAGE PROCESSING; METABOLISM; PHYSIOLOGY; PROCEDURES;

ANIMALS; CARDIAC RESYNCHRONIZATION THERAPY; DEPENDOVIRUS; FEMALE; HEART; IMAGE PROCESSING, COMPUTER-ASSISTED; MALE; OPTICAL IMAGING; OPTOGENETICS; RATS; RHODOPSIN;

EID: 84953398735     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.3268     Document Type: Article

References (22)

1. Boyden, E.S., Zhang, F., Bamberg, E., Nagel, G. , Deisseroth, K. Millisecond-timescale, genetically targeted optical control of neural activity. Nat. Neurosci. 8, 1263-1268 (2005).
2. Deisseroth, K. Optogenetics. Nat. Methods 8, 26-29 (2011).
3. Kravitz, A.V. , Kreitzer, A.C. Optogenetic manipulation of neural circuitry in vivo. Curr. Opin. Neurobiol. 21, 433-439 (2011).
4. Szobota, S. , Isacoff, E.Y. Optical control of neuronal activity. Annu. Rev. Biophys. 39, 329-348 (2010).
5. Bristow, M.R. et al. Cardiac-resynchronization therapy with or without an implantable defibrillator in advanced chronic heart failure. N. Engl. J. Med. 350, 2140-2150 (2004).
6. Cho, H.C. , Marban, E. Biological therapies for cardiac arrhythmias: can genes and cells replace drugs and devices? Circ. Res. 106, 674-685 (2010).
7. Rosen, M.R., Robinson, R.B., Brink, P.R. , Cohen, I.S. The road to biological pacing. Nat. Rev. Cardiol. 8, 656-666 (2011).
8. Nagel, G. et al. Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc. Natl. Acad. Sci. USA 100, 13940-13945 (2003).
9. Jia, Z. et al. Stimulating cardiac muscle by light: cardiac optogenetics by cell delivery. Circ. Arrhythm. Electrophysiol. 4, 753-760 (2011).
10. Nussinovitch, U., Shinnawi, R. , Gepstein, L. Modulation of cardiac tissue electrophysiological properties with light-sensitive proteins. Cardiovasc. Res. 102, 176-187 (2014).
11. Arrenberg, A.B., Stainier, D.Y., Baier, H. , Huisken, J. Optogenetic control of cardiac function. Science 330, 971-974 (2010).
12. Bruegmann, T. et al. Optogenetic control of heart muscle in vitro and in vivo. Nat. Methods 7, 897-900 (2010).
13. Boyle, P.M., Williams, J.C., Ambrosi, C.M., Entcheva, E. , Trayanova, N.A. A comprehensive multiscale framework for simulating optogenetics in the heart. Nat. Commun. 4, 2370 (2013).
14. Inagaki, K. et al. Robust systemic transduction with AAV9 vectors in mice: efficient global cardiac gene transfer superior to that of AAV8. Mol. Ther. 14, 45-53 (2006).
15. Wilkoff, B.L. et al. Dual-chamber pacing or ventricular backup pacing in patients with an implantable defibrillator: the Dual Chamber and VVI Implantable Defibrillator (DAVID) Trial. J. Am. Med. Assoc. 288, 3115-3123 (2002).
16. Miake, J., Marban, E. , Nuss, H.B. Biological pacemaker created by gene transfer. Nature 419, 132-133 (2002).
17. Plotnikov, A.N. et al. Biological pacemaker implanted in canine left bundle branch provides ventricular escape rhythms that have physiologically acceptable rates. Circulation 109, 506-512 (2004).
18. Kapoor, N., Liang, W., Marban, E. , Cho, H.C. Direct conversion of quiescent cardiomyocytes to pacemaker cells by expression of Tbx18. Nat. Biotechnol. 31, 54-62 (2012).
19. Kehat, I. et al. Electromechanical integration of cardiomyocytes derived from human embryonic stem cells. Nat. Biotechnol. 22, 1282-1289 (2004).
20. Plotnikov, A.N. et al. Xenografted adult human mesenchymal stem cells provide a platform for sustained biological pacemaker function in canine heart. Circulation 116, 706-713 (2007).
21. Potapova, I. et al. Human mesenchymal stem cells as a gene delivery system to create cardiac pacemakers. Circ. Res. 94, 952-959 (2004).
22. Lin, J.Y., Knutsen, P.M., Muller, A., Kleinfeld, D. , Tsien, R.Y. ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. Nat. Neurosci. 16, 1499-1508 (2013).