Detection and tracking of dual-labeled hiv particles using wide-field live cell imaging to follow viral core integrity

Methods in Molecular Biology

Volumn 1354, Issue , 2016, Pages 49-59

  Mamede, João I ^a   Hope, Thomas J ^a  

^a NONE

Author keywords

HIV early steps of infection; HIV fusion; HIV uncoating; HIV viral core; Live cell imaging; Wide field microscopy

Indexed keywords

BLEACHING; CONTROLLED STUDY; DATA ANALYSIS; DATA ANALYSIS SOFTWARE; FLUORESCENCE MICROSCOPY; HEK293 CELL LINE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN IMMUNODEFICIENCY VIRUS; MOLECULAR IMAGING; NONHUMAN; PHOTOTOXICITY; SIGNAL NOISE RATIO; T LYMPHOCYTE; VIRION; VIRUS CORE; VIRUS DETECTION; VIRUS PARTICLE; CELL CULTURE TECHNIQUE; CELL LINE; FLUORESCENCE IMAGING; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; ISOLATION AND PURIFICATION; MICROSCOPY; PATHOLOGY; PHYSIOLOGY; PROCEDURES; SOFTWARE; STAINING; VIROLOGY; VIRUS UNCOATING;

CELL CULTURE TECHNIQUES; CELL LINE; HIV INFECTIONS; HIV-1; HUMANS; MICROSCOPY; OPTICAL IMAGING; SOFTWARE; STAINING AND LABELING; VIRUS UNCOATING;

EID: 84952837992     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3046-3_4     Document Type: Chapter

References (15)

1. Fassati A, Goff SP (2001) Characterization of intracellular reverse transcription complexes of human immunodefi ciency virus type 1. J Virol 75:3626–3635. doi: 10.1128/JVI.75.8.3626-3635.2001
2. Goff SP (2001) Intracellular trafficking of retroviral genomes during the early phase of infection: viral exploitation of cellular pathways. J Gene Med 3:517–528. doi: 10.1002/1521-2254(200111)3:6<517::AIDJGM234>3.0.CO;2-E
3. McDonald D, Vodicka MA, Lucero G, Svitkina TM, Borisy GG, Emerman M, Hope TJ (2002) Visualization of the intracellular behavior of HIV in living cells. J Cell Biol 159:441–452. doi: 10.1083/jcb.200203150
4. Hulme AE, Perez O, Hope TJ (2011) Complementary assays reveal a relationship between HIV-1 uncoating and reverse transcription. Proc Natl Acad Sci U S A 108:9975–9980. doi: 10.1073/pnas.1014522108
5. Nermut MV, Fassati A (2003) Structural analyses of purifi ed human immunodefi ciency virus type 1 intracellular reverse transcription complexes. J Virol 77:8196–8206
6. Warrilow D, Tachedjian G, Harrich D (2009) Maturation of the HIV reverse transcription complex: putting the jigsaw together. Rev Med Virol 19:324–337. doi: 10.1002/rmv.627
7. Xu H, Franks T, Gibson G, Huber K, Rahm N, De Castillia CS, Luban J, Aiken C, Watkins S, Sluis-Cremer N, Ambrose Z (2013) Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay. Retrovirology 10:70. doi: 10.1186/1742-4690-10-70
8. Hübner W, Chen P, Portillo AD, Liu Y, Gordon RE, Chen BK (2007) Sequence of human immunodefi ciency virus type 1 (HIV- 1) gag localization and oligomerization monitored with live confocal imaging of a replicationcompetent, fl uorescently tagged HIV-1. J Virol 81:12596–12607. doi: 10.1128/JVI.01088-07
9. Yu Z, Dobro MJ, Woodward CL, Levandovsky A, Danielson CM, Sandrin V, Shi J, Aiken C, Zandi R, Hope TJ, Jensen GJ (2013) Unclosed HIV-1 capsids suggest a curled sheet model of assembly. J Mol Biol 425:112–123. doi: 10.1016/j.jmb.2012.10.006
10. Padilla-Parra S, Marin M, Gahlaut N, Suter R, Kondo N, Melikyan GB (2013) Fusion of mature HIV-1 particles leads to complete release of a Gag-GFP-based content marker and raises the intraviral pH. PLoS One 8, e71002. doi: 10.1371/journal.pone.0071002
11. Finkelshtein D, Werman A, Novick D, Barak S, Rubinstein M (2013) LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus. Proc Natl Acad Sci U S A 110:7306–7311. doi: 10.1073/pnas.1214441110
12. Jaqaman K, Loerke D, Mettlen M, Kuwata H, Grinstein S, Schmid SL, Danuser G (2008) Robust single-particle tracking in live-cell time- lapse sequences. Nat Methods 5:695–702. doi: 10.1038/nmeth.1237
13. Diaspro, A., Chirico, G., Usai, C., Ramoino, P., and Dobrucki, J. (2006) Photobleaching. In: Pawley JB (ed) Handb. Biol. Confocal Microsc. Springer US, pp 690–702
14. Tinevez J-Y, Dragavon J, Baba-Aissa L, Rox P, Perret E, Canivet A, Galy V, Shorte S (2012) Chapter fifteen-a quantitative method for measuring phototoxicity of a live cell imaging microscope. In: Conn M (ed) Methods enzymol. Academic, New York, pp 291–309
15. Zhang Y, Hatziioannou T, Zang T, Braaten D, Luban J, Goff SP, Bieniasz PD (2002) Envelope-dependent, cyclophilin-independent effects of glycosaminoglycans on human immunodefi ciency virus type 1 attachment and infection. J Virol 76:6332–6343. doi: 10.1128/JVI.76.12.6332-6343.2002