Col2-Cre and tamoxifen-inducible Col2-CreER target different cell populations in the knee joint

Osteoarthritis and Cartilage

Volumn 24, Issue 1, 2016, Pages 188-191

  Nagao, M ^a   Cheong, C W ^a   Olsen, B R ^a  

^a HARVARD SCHOOL OF DENTAL MEDICINE   (United States)

Author keywords

Col2 Cre; Col2 CreER; Osteoarthritis; Tamoxifen

Indexed keywords

COLLAGEN TYPE 2; TAMOXIFEN; PHOTOPROTEIN;

ANIMAL TISSUE; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CONTROLLED STUDY; EPIPHYSIS; GROWTH PLATE; KNEE; KNEE MENISCUS; KNEE OSTEOARTHRITIS; LIGAMENT; MOUSE; NONHUMAN; PRIORITY JOURNAL; SYNOVIUM; TRANSGENIC MOUSE; ANIMAL; BONE; CHONDROCYTE; EPIPHYSIS PLATE; METABOLISM; REPORTER GENE; STIFLE;

ANIMALS; BONE AND BONES; CARTILAGE, ARTICULAR; CHONDROCYTES; COLLAGEN TYPE II; GENES, REPORTER; GROWTH PLATE; KNEE JOINT; LUMINESCENT PROTEINS; MICE; MICE, TRANSGENIC; STIFLE; TAMOXIFEN;

EID: 84951806319     PISSN: 10634584     EISSN: 15229653     Source Type: Journal    
DOI: 10.1016/j.joca.2015.07.025     Document Type: Article

References (13)

1. Sakai K., Hiripi L., Glumoff V., Brandau O., Eerola R., Vuorio E., et al. Stage-and tissue-specific expression of a Col2a1-Cre fusion gene in transgenic mice. Matrix Biol 2001, 19:761-767. pii: S0945053X00001220.
2. Fosang A.J., Golub S.B., East C.J., Rogerson F.M. Abundant LacZ activity in the absence of Cre expression in the normal and inflamed synovium of adult Col2a1-Cre; ROSA26RLacZ reporter mice. Osteoarthritis Cartilage 2013, 21:401-404. 10.1016/j.joca.2012.11.013.
3. Ono N., Ono W., Nagasawa T., Kronenberg H.M. A subset of chondrogenic cells provides early mesenchymal progenitors in growing bones. Nat Cell Biol 2014, 16:1157-1167. 10.1038/ncb3067.
4. Long F., Zhang X.M., Karp S., Yang Y., McMahon A.P. Genetic manipulation of hedgehog signaling in the endochondral skeleton reveals a direct role in the regulation of chondrocyte proliferation. Development 2001, 128:5099-5108.
5. Nakamura E., Nguyen M.T., Mackem S. Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreER(T) to assay temporal activity windows along the proximodistal limb skeleton. Dev Dyn 2006, 235:2603-2612. 10.1002/dvdy.20892.
6. Little C.B., Hunter D.J. Post-traumatic osteoarthritis: from mouse models to clinical trials. Nat Rev Rheumatol 2013, 9:485-497. 10.1038/nrrheum.2013.72.
7. Yang L., Tsang K.Y., Tang H.C., Chan D., Cheah K.S. Hypertrophic chondrocytes can become osteoblasts and osteocytes in endochondral bone formation. Proc Natl Acad Sci USA 2014, 111:12097-12102. 10.1073/pnas.1302703111.
8. Grimmer C., Balbus N., Lang U., Aigner T., Cramer T., Muller L., et al. Regulation of type II collagen synthesis during osteoarthritis by prolyl-4-hydroxylases: possible influence of low oxygen levels. Am J Pathol 2006, 169:491-502. pii: S0002-9440(10)62732-0.
9. Chen M., Lichtler A.C., Sheu T.J., Xie C., Zhang X., O'Keefe R.J., et al. Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase. Genesis 2007, 45:44-50. 10.1002/dvg.20261.
10. Hilton M.J., Tu X., Long F. Tamoxifen-inducible gene deletion reveals a distinct cell type associated with trabecular bone, and direct regulation of PTHrP expression and chondrocyte morphology by Ihh in growth region cartilage. Dev Biol 2007, 308:93-105. pii: S0012-1606(07)00905-0.
11. Wang M., Sampson E.R., Jin H., Li J., Ke Q.H., Im H.J., et al. MMP13 is a critical target gene during the progression of osteoarthritis. Arthritis Res Ther 2013, 15:R5. 10.1186/ar4133.
12. Sugita S., Hosaka Y., Okada K., Mori D., Yano F., Kobayashi H., et al. Transcription factor Hes1 modulates osteoarthritis development in cooperation with calcium/calmodulin-dependent protein kinase 2. Proc Natl Acad Sci USA 2015, 112:3080-3085. 10.1073/pnas.1419699112.
13. Weng T., Yi L., Huang J., Luo F., Wen X., Du X., et al. Genetic inhibition of fibroblast growth factor receptor 1 in knee cartilage attenuates the degeneration of articular cartilage in adult mice. Arthritis Rheum 2012, 64:3982-3992. 10.1002/art.34645.