Abundant LacZ activity in the absence of Cre expression in the normal and inflamed synovium of adult Col2a1-Cre; ROSA26RLacZ reporter mice

Osteoarthritis and Cartilage

Volumn 21, Issue 2, 2013, Pages 401-404

  Fosang, A J ^a   Golub, S B ^a   East, C J ^a   Rogerson, F M ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Col2a1; Col2a1 Cre; Cre recombinase; Inflammatory arthritis; Synovium

Indexed keywords

ALANINE; ARGININE; BETA GALACTOSIDASE; COLLAGEN TYPE 2; COLLAGEN TYPE 2A1; CRE RECOMBINASE; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANIMAL EXPERIMENT; ARTHRITIS; ARTICLE; CELL MATURATION; CONTROLLED STUDY; ENZYME ACTIVITY; FIBROBLAST; GENE EXPRESSION; GENETIC RECOMBINATION; IMMUNOHISTOCHEMISTRY; JOINT; MEDIATOR; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; SYNOVIOCYTE; SYNOVITIS; SYNOVIUM;

ANIMALS; ARTHRITIS; COLLAGEN TYPE II; DISEASE MODELS, ANIMAL; FIBROBLASTS; GENE EXPRESSION REGULATION; GENES, REPORTER; INTEGRASES; KNEE JOINT; LAC OPERON; MICE; MICE, TRANSGENIC; PROTEINS; SYNOVIAL MEMBRANE; TIME FACTORS;

EID: 84872669347     PISSN: 10634584     EISSN: 15229653     Source Type: Journal    
DOI: 10.1016/j.joca.2012.11.013     Document Type: Article

References (11)

1. Sakai K., Hiripi L., Glumoff V., Brandau O., Eerola R., Vuorio E., et al. Stage-and tissue-specific expression of a Col2a1-Cre fusion gene in transgenic mice. Matrix Biol 2001, 19:761-767.
2. Ovchinnikov D.A., Deng J.M., Ogunrinu G., Behringer R.R. Col2a1-directed expression of Cre recombinase in differentiating chondrocytes in transgenic mice. Genesis 2000, 26:145-146.
3. Soriano P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 1999, 21:70-71.
4. Mao X., Fujiwara Y., Orkin S.H. Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A 1999, 96:5037-5042.
5. Hyde G., Dover S., Aszodi A., Wallis G.A., Boot-Handford R.P. Lineage tracing using matrilin-1 gene expression reveals that articular chondrocytes exist as the joint interzone forms. Dev Biol 2007, 304:825-833.
6. Koyama E., Shibukawa Y., Nagayama M., Sugito H., Young B., Yuasa T., et al. A distinct cohort of progenitor cells participates in synovial joint and articular cartilage formation during mouse limb skeletogenesis. Dev Biol 2008, 316:62-73.
7. Hyde G., Boot-Handford R.P., Wallis G.A. Col2a1 lineage tracing reveals that the meniscus of the knee joint has a complex cellular origin. J Anat 2008, 213:531-538.
8. Stanton H., Rogerson F.M., East C.J., Golub S.B., Lawlor K.E., Meeker C.T., et al. ADAMTS5 is the major aggrecanase in mouse cartilage in vivo and in vitro. Nature 2005, 434:648-652.
9. Pacifici M., Koyama E., Shibukawa Y., Wu C., Tamamura Y., Enomoto-Iwamoto M., et al. Cellular and molecular mechanisms of synovial joint and articular cartilage formation. Ann N Y Acad Sci 2006, 1068:74-86.
10. Nakamura E., Nguyen M.T., Mackem S. Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreER(T) to assay temporal activity windows along the proximodistal limb skeleton. Dev Dyn 2006, 235:2603-2612.
11. Chen M., Lichtler A.C., Sheu T.J., Xie C., Zhang X., O'Keefe R.J., et al. Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase. Genesis 2007, 45:44-50.