Anti-breast tumor activity of Eclipta extract in-vitro and in-vivo: Novel evidence of endoplasmic reticulum specific localization of Hsp60 during apoptosis

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Arya, Rakesh K ^a   Singh, Akhilesh ^a   Yadav, Navneet K ^a   Cheruvu, Srikanth H ^a   Hossain, Zakir ^a   Meena, Sanjeev ^a   Maheshwari, Shrankhla ^a,b   Singh, Anup K ^a   Shahab, Uzma ^a   Sharma, Chetan ^a   Singh, Kavita ^a   Narender, Tadigoppula ^a   Mitra, Kalyan ^a   Arya, Kamal R ^a   Singh, Rama K ^a   Gayen, Jiaur R ^a   Datta, Dipak ^a,b  

^a CENTRAL DRUG RESEARCH INSTITUTE   (India)

^b ACADEMY OF SCIENTIFIC AND INNOVATIVE RESEARCH ACSIR   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC AGENT; CHAPERONIN 60; CHLOROFORM; PLANT EXTRACT; X LINKED INHIBITOR OF APOPTOSIS;

ANIMAL; APOPTOSIS; BAGG ALBINO MOUSE; BREAST NEOPLASMS; CELL LINE; CELL SURVIVAL; CHEMISTRY; CONFOCAL MICROSCOPY; DRUG EFFECTS; ECLIPTA; ENDOPLASMIC RETICULUM; FEMALE; GENETICS; HUMAN; MAMMARY NEOPLASMS, EXPERIMENTAL; MCF-7 CELL LINE; METABOLISM; MITOCHONDRIAL MEMBRANE POTENTIAL; PATHOLOGY; PHYTOTHERAPY; PROCEDURES; RNA INTERFERENCE; SIGNAL TRANSDUCTION; TUMOR CELL LINE; WESTERN BLOTTING;

ANIMALS; ANTINEOPLASTIC AGENTS; APOPTOSIS; BLOTTING, WESTERN; BREAST NEOPLASMS; CELL LINE; CELL LINE, TUMOR; CELL SURVIVAL; CHAPERONIN 60; CHLOROFORM; ECLIPTA; ENDOPLASMIC RETICULUM; FEMALE; HUMANS; MAMMARY NEOPLASMS, EXPERIMENTAL; MCF-7 CELLS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MICE, INBRED BALB C; MICROSCOPY, CONFOCAL; PHYTOTHERAPY; PLANT EXTRACTS; RNA INTERFERENCE; SIGNAL TRANSDUCTION; X-LINKED INHIBITOR OF APOPTOSIS PROTEIN;

EID: 84950315734     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep18457     Document Type: Article

References (50)

1. Ferlay, J. et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. International Journal of Cancer 136, E359-E386 (2015).
2. Cragg, G. M. & Newman, D. J. Natural products: A continuing source of novel drug leads. Biochim Biophys Acta 1830, 3670-3695 (2013).
3. Kaur, M., Singh, R. P., Gu, M., Agarwal, R. & Agarwal, C. Grape seed extract inhibits in vitro and in vivo growth of human colorectal carcinoma cells. Clin Cancer Res 12, 6194-6202 (2006).
4. Widodo, N. et al. Selective killing of cancer cells by leaf extract of Ashwagandha: Identification of a tumor-inhibitory factor and the first molecular insights to its effect. Clinical Cancer Research 13, 2298-2306 (2007).
5. Lee, K. W., Bode, A. M. & Dong, Z. Molecular targets of phytochemicals for cancer prevention. Nat Rev Cancer 11, 211-218 (2011).
6. Newman, D. J. & Cragg, G. M. Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod 75, 311-335 (2012).
7. Nagalingam, A., Kuppusamy, P., Singh, S. V., Sharma, D. & Saxena, N. K. Mechanistic elucidation of the antitumor properties of withaferin a in breast cancer. Cancer Res 74, 2617-2629 (2014).
8. Organization WH. WHO guidelines on safety monitoring of herbal medicines in pharmacovigilance systems. (2004).
9. Saxena, A. K., Singh, B. & Anand, K. K. Hepatoprotective effects of Eclipta alba on subcellular levels in rats. J Ethnopharmacol 40, 155-161 (1993).
10. Chaudhary, H., Dhuna, V., Singh, J., Kamboj, S. S. & Seshadri, S. Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: An in vitro study. J Ethnopharmacol 136, 363-367 (2011).
11. Mukherjee, S. et al. Green chemistry approach for the synthesis and stabilization of biocompatible gold nanoparticles and their potential applications in cancer therapy. Nanotechnology 23, 455103 (2012).
12. Wagner, H., Geyer, B., Kiso, Y., Hikino, H. & Rao, G. S. Coumestans as the Main Active Principles of the Liver Drugs Eclipta alba and Wedelia calendulacea. Planta Medica 52, 370-374 (1986).
13. Jadhav, V., Thorat, R., Kadam, V. & Salaskar, K. Chemical composition, pharmacological activities of Eclipta alba. Journal of Pharmacy Research 2, 1229-1231 (2009).
14. Singh, B., Saxena, A. K., Chandan, B. K., Agarwal, S. G. & Anand, K. K. In vivo hepatoprotective activity of active fraction from ethanolic extract of Eclipta alba leaves. Indian J Physiol Pharmacol 45, 435-441 (2001).
15. Lin, Y., Shi, R., Wang, X. & Shen H-M., Luteolin, a flavonoid with potentials for cancer prevention and therapy. Current cancer drug targets 8, 634 (2008).
16. Ren, W., Qiao, Z., Wang, H., Zhu, L. & Zhang, L. Flavonoids: Promising anticancer agents. Med Res Rev 23, 519-534 (2003).
17. Tsai, C. H. et al. Herbal extract of Wedelia chinensis attenuates androgen receptor activity and orthotopic growth of prostate cancer in nude mice. Clin Cancer Res 15, 5435-5444 (2009).
18. Green, D. R. & Kroemer, G. The pathophysiology of mitochondrial cell death. Science 305, 626-629 (2004).
19. Soltys, B. J. & Gupta, R. S. Immunoelectron microscopic localization of the 60-kDa heat shock chaperonin protein (Hsp60) in mammalian cells. Experimental cell research 222, 16-27 (1996).
20. Cappello, F., Conway de Macario, E., Marasà, L., Zummo, G. & Macario, A. J. Hsp60 expression, new locations, functions and perspectives for cancer diagnosis and therapy. Cancer Biol Ther 7, 801-809 (2008).
21. Cappello, F. et al. 60KDa chaperonin (HSP60) is over-expressed during colorectal carcinogenesis. Eur J Histochem 47, 105-110 (2003).
22. Beere, H. M. Death versus survival: Functional interaction between the apoptotic and stress-inducible heat shock protein pathways. J Clin Invest 115, 2633-2639 (2005).
23. Chandra, D., Choy, G. & Tang, D. G. Cytosolic accumulation of HSP60 during apoptosis with or without apparent mitochondrial release: Evidence that its pro-apoptotic or pro-survival functions involve differential interactions with caspase-3. J Biol Chem 282, 31289-31301 (2007).
24. Ghosh, J. C., Siegelin, M. D., Dohi, T. & Altieri, D. C. Heat shock protein 60 regulation of the mitochondrial permeability transition pore in tumor cells. Cancer Res 70, 8988-8993 (2010).
25. Ghosh, J. C., Dohi, T., Kang, B. H. & Altieri, D. C. Hsp60 regulation of tumor cell apoptosis. J Biol Chem 283, 5188-5194 (2008).
26. Xanthoudakis, S. et al. Hsp60 accelerates the maturation of pro-caspase-3 by upstream activator proteases during apoptosis. EMBO J 18, 2049-2056 (1999).
27. Samali, A., Cai, J., Zhivotovsky, B., Jones, D. P. & Orrenius, S. Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells. EMBO J 18, 2040-2048 (1999).
28. Datta, D., Banerjee, P., Gasser, M., Waaga-Gasser, A. M. & Pal, S. CXCR3-B can mediate growth-inhibitory signals in human renal cancer cells by down-regulating the expression of heme oxygenase-1. J Biol Chem 285, 36842-36848 (2010).
29. Fulda, S. & Debatin, K. M. Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene 25, 4798-4811 (2006).
30. Cechetto, J. D., Soltys, B. J. & Gupta, R. S. Localization of mitochondrial 60-kD heat shock chaperonin protein (Hsp60) in pituitary growth hormone secretory granules and pancreatic zymogen granules. J Histochem Cytochem 48, 45-56 (2000).
31. Leventis, P. A. & Grinstein, S. The distribution and function of phosphatidylserine in cellular membranes. Annual review of biophysics 39, 407-427 (2010).
32. Pawar, V. K. et al. Immunotherapeutic vitamin E nanoemulsion synergies the antiproliferative activity of paclitaxel in breast cancer cells via modulating Th1 and Th2 immune response. Journal of Controlled Release 196, 295-306 (2014).
33. Ji, H. F., Li, X. J. & Zhang, H. Y. Natural products and drug discovery. EMBO reports 10, 194-200 (2009).
34. Garraway, L. A. & Jänne, P. A. Circumventing cancer drug resistance in the era of personalized medicine. Cancer discovery 2, 214-226 (2012).
35. Singh, A. K. et al. Tumor heterogeneity and cancer stem cell paradigm: Updates in concept, controversies and clinical relevance. International Journal of Cancer 136, 1991-2000 (2015).
36. Hanahan, D. & Weinberg, R. A. Hallmarks of cancer: The next generation. Cell 144, 646-674 (2011).
37. Munoz-Pinedo, C., El Mjiyad, N. & Ricci, J. Cancer metabolism: Current perspectives and future directions. Cell death & disease 3, e248 (2012).
38. Gogvadze, V., Orrenius, S. & Zhivotovsky, B. Mitochondria in cancer cells: What is so special about them? Trends in Cell Biology 18, 165-173.
39. Gupta, S. & Knowlton, A. A. Cytosolic heat shock protein 60, hypoxia, and apoptosis. Circulation 106, 2727-2733 (2002).
40. Kim, H. S. et al. Heat shock protein 60 modified with O-linked N-acetylglucosamine is involved in pancreatic ?-cell death under hyperglycemic conditions. FEBS letters 580, 2311-2316 (2006).
41. Hayoun, D. et al. HSP60 is transported through the secretory pathway of 3-MCA-induced fibrosarcoma tumour cells and undergoes N-glycosylation. FEBS J 279, 2083-2095 (2012).
42. Seelinger, G., Merfort, I., Wölfle, U. & Schempp, C. M. Anti-carcinogenic effects of the flavonoid luteolin. Molecules 13, 2628-2651 (2008).
43. Shi, R. X., Ong, C. N. & Shen, H. M. Protein kinase C inhibition and x-linked inhibitor of apoptosis protein degradation contribute to the sensitization effect of luteolin on tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in cancer cells. Cancer Res 65, 7815-7823 (2005).
44. Vichai, V. & Kirtikara, K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc 1, 1112-1116 (2006).
45. Chauhan, S. S. et al. Synthesis of novel ?-carboline based chalcones with high cytotoxic activity against breast cancer cells. Bioorganic & medicinal chemistry letters 24, 2820-2824 (2014).
46. Cossarizza, A., Baccarani-Contri, M., Kalashnikova, G. & Franceschi, C. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5? ,6,6?-tetrachloro-1,1? ,3,3?-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem Biophys Res Commun 197, 40-45 (1993).
47. Wieckowski, M. R., Giorgi, C., Lebiedzinska, M., Duszynski, J. & Pinton, P. Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells. Nature protocols 4, 1582-1590 (2009).
48. Datta, D. et al. Ras-induced modulation of CXCL10 and its receptor splice variant CXCR3-B in MDA-MB-435 and MCF-7 cells: Relevance for the development of human breast cancer. Cancer Res 66, 9509-9518 (2006).
49. Tuominen, V. J., Ruotoistenmaki, S., Viitanen, A., Jumppanen, M. & Isola, J. ImmunoRatio: A publicly available web application for quantitative image analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67. Breast Cancer Res 12, R56 (2010).
50. Datta, D. et al. Calcineurin inhibitors activate the proto-oncogene Ras and promote protumorigenic signals in renal cancer cells. Cancer Res 69, 8902-8909 (2009).