Visualizing G protein-coupled receptor-receptor interactions in brain using proximity ligation in situ assay

Current Protocols in Cell Biology

Volumn 2015, Issue , 2015, Pages 17.17.1-17.17.16

  Taura, Jaume ^a   Fernández Dueñas, Víctor ^a   Ciruela, Francisco ^a,b  

^a UNIVERSITY OF BARCELONA   (Spain)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Brain; GPCR; Oligomerization; Proximity ligation assay

Indexed keywords

G PROTEIN COUPLED RECEPTOR; OLIGOMER; PROTEIN BINDING;

ANIMAL TISSUE; ARTICLE; BIOASSAY; BRAIN; CONTROLLED STUDY; INVESTIGATIVE PROCEDURES; MOLECULAR IMAGING; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; PROCESS DESIGN; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; PROXIMITY LIGATION IN SITU ASSAY; SENSITIVITY ANALYSIS; VALIDATION STUDY; ANIMAL; IMAGE PROCESSING; METABOLISM; MOUSE; PROCEDURES; TISSUE FIXATION;

ANIMALS; BIOLOGICAL ASSAY; BRAIN; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; PROTEIN BINDING; RECEPTORS, G-PROTEIN-COUPLED; TISSUE FIXATION;

EID: 84949294350     PISSN: 19342500     EISSN: 19342616     Source Type: Journal    
DOI: 10.1002/0471143030.cb1717s67     Document Type: Article

References (20)

1. Agnati, L.F., Fuxe, K., Zini, I., Lenzi, P., and Hokfelt, T. 1980. Aspects on receptor regulation and isoreceptor identification. Med. Biol. 58:182-187.
2. Albizu, L., Cottet, M., Kralikova, M., Stoev, S., Seyer, R., Brabet, I., Roux, T., Bazin, H., Bourrier, E., Lamarque, L., Breton, C., Rives, M.L., Newman, A., Javitch, J., Trinquet, E., Manning, M., Pin, J.P., Mouillac, B., and Durroux, T. 2010. Time-resolved FRET between GPCR ligands reveals oligomers in native tissues. Nat. Chem. Biol. 6:587-594.
3. Besmer, P., Miller, R.C. Jr., Caruthers, M.H., Kumar, A., Minamoto, K., Van de Sande, J.H., Sidarova, N., and Khorana, H.G. 1972. Studies on polynucleotides. CXVII. Hybridization of polydeoxynucleotides with tyrosine transfer RNA sequences to the r-strand of phi80psu + 3 DNA. J. Mol. Biol. 72:503-522.
4. Clark, J.D., Gebhart, G.F., Gonder, J.C., Keeling, M.E., and Kohn, D.F. 1997. Special report: The 1996 guide for the care and use of laboratory animals. ILAR J. 38:41-48.
5. Fernández-Dueñas, V., Taura, J.J., Cottet, M., Gómez-Soler, M., López-Cano, M., Ledent, C., Watanabe, M., Trinquet, E., Pin, J.-P., Luján, R., Durroux, T., and Ciruela, F. 2015. Untangling dopamine-adenosine receptor-receptor assembly in experimental parkinsonism in rats. Dis. Model. Mech. 8:57-63.
6. Flecknell, P.A. 1993. Anaesthesia of animals for biomedical research. Br. J. Anaesth. 71:885-894.
7. Fredriksson, S., Gullberg, M., Jarvius, J., Olsson, C., Pietras, K., Gústafsdóttir, S.M., Ostman, A., and Landegren, U. 2002. Protein detection using proximity-dependent DNA ligation assays. Nat. Biotechnol. 20:473-477.
8. Fuxe, K., Agnati, L.F., Benfenati, F., Celani, M., Zini, I., Zoli, M., and Mutt, V. 1983. Evidence for the existence of receptor-receptor interactions in the central nervous system. Studies on the regulation of monoamine receptors by neuropeptides. J. Neural Transm. 18:165-179.
9. Jarvius, M., Paulsson, J., Weibrecht, I., Leuchowius, K.-J., Andersson, A.-C., Wählby, C., Gullberg, M., Botling, J., Sjöblom, T., Markova, B., Ostman, A., Landegren, U., and Söderberg, O. 2007. In situ detection of phosphorylated platelet-derived growth factor receptor ß using a generalized proximity ligation method. Mol. Cell Proteomics 6:1500-1509.
10. Matamales, M., Bertran-Gonzalez, J., Salomon, L., Degos, B., Deniau, J., Valjent, E., Hervé, D., and Girault, J. 2009. Striatal medium-sized spiny neurons: Identification by nuclear staining and study of neuronal subpopulations in BAC transgenic mice. PLoS One 4:e4770.
11. Miller, J. and Stagljar, I. 2004. Using the yeast twohybrid system to identify interacting proteins. Methods Mol. Biol. 261:247-262.
12. Palczewski, K. 2006. G protein-coupled receptor rhodopsin. Annu. Rev. Biochem. 75:743-767.
13. Ramos-Vara, J.A. and Miller, M.A. 2014. When tissue antigens and antibodies get along: Revisiting the technical aspects of immunohistochemistry-the red, brown, and blue technique. Vet. Pathol. 51:42-87.
14. Ritter, S.L. and Hall, R.A. 2009. Fine-tuning of GPCR activity by receptor-interacting proteins. Nat. Rev. Mol. Cell Biol. 10:819-830.
15. Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.Y., White, D.J., Hartenstein, V., Eliceiri, K., Tomancak, P., and Cardona, A. 2012. Fiji: An open-source platform for biological-image analysis. Nat. Methods 9:676-982.
16. Selbach, M. and Mann, M. 2006. Protein interaction screening by quantitative immunoprecipitation combined with knockdown (QUICK). Nat. Methods 3:981-983.
17. Smith, C.L. 2011. Basic confocal microscopy. Curr. Protoc. Neurosci. 56:2.2.1-2.2.18.
18. Söderberg, O., Leuchowius, K.-J., Gullberg, M., Jarvius, M., Weibrecht, I., Larsson, L.-G., and Landegren, U. 2008. Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay. Methods 45:227-232.
19. Söderberg, O., Gullberg, M., Jarvius, M., Ridderstrale, K., Leuchowius, K.-J., Jarvius, J., Wester, K., Hydbring, P., Bahram, F., Larsson, L.-G., and Landegren, U. 2006. Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat. Methods 3:995-1000.
20. Trifilieff, P., Rives, M.-L., Urizar, E., Piskorowski, R.A., Vishwasrao, H.D., Castrillon, J., Schmauss, C., Slättman, M., Gullberg, M., and Javitch, J.A. 2011. Detection of antigen interactions ex vivo by proximity ligation assay: Endogenous dopamine D2-adenosine A2A receptor complexes in the striatum. Biotechniques 51:111-118.