Genetic manipulation of acinetobacter baumannii

Current Protocols in Microbiology

Volumn 2014, Issue , 2014, Pages 6G.2.1-6G.2.11

  Jacobs, Anna C ^a   Thompson, Mitchell G ^a   Gebhardt, Michael ^b   Corey, Brendan W ^a   Yildirim, Suleyman ^a   Shuman, Howard A ^b   Zurawski, Daniel V ^a  

^a WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

Acinetobacter baumannii; Conjugation; Electroporation; Mutagenesis; Plasmid; Transformation

Indexed keywords

AGAR; PHOSPHATE BUFFERED SALINE; BACTERIAL DNA;

ACINETOBACTER BAUMANNII; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL; BACTERIUM CONJUGATION; BACTERIUM TRANSFORMATION; BROTH DILUTION; CELL MOTILITY; CULTURE MEDIUM; DNA TRANSFER; ELECTROPORATION; GENE REPLICATION; GENETIC MANIPULATION; LYSOGENY BROTH; MUTAGENESIS; NONHUMAN; PLASMID; PRIORITY JOURNAL; SOC MEDIUM; GENE EXPRESSION REGULATION; GENE TRANSFER; GENETICS; MICROBIOLOGICAL EXAMINATION; PHYSIOLOGY;

ACINETOBACTER BAUMANNII; BACTERIOLOGICAL TECHNIQUES; CONJUGATION, GENETIC; DNA, BACTERIAL; ELECTROPORATION; GENE EXPRESSION REGULATION, BACTERIAL; GENE TRANSFER TECHNIQUES;

EID: 84949233456     PISSN: 19348525     EISSN: 19348533     Source Type: Journal    
DOI: 10.1002/9780471729259.mc06g02s35     Document Type: Article

References (23)

1. Amin, I.M., Richmond, G.E., Sen, P., Koh, T.H., Piddock, L.J., and Chua, K.L. 2013. A method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii BMC Microbiol. 13:158.
2. Aranda, J., Poza, M., Pardo, B.G., Rumbo, S., Rumbo, C., Parreira, J.R., Rodŕıguez-Velo, P., and Bou, G. 2010. A rapid and simple method for constructing stable mutants of Acinetobacter baumannii. BMC Microbiol. 10:279.
3. Bryksin, A.V. and Matsumura, I. 2010. Rational design of a plasmid origin that replicates efficiently in both Gram-positive and Gram-negative bacteria PLoS One 5:e13244.
4. Camarena, L., Bruno, V., Euskirchen, G., Poggio, S., and Snyder, M. 2010. Molecular mechanisms of ethanol-induced pathogenesis revealed byRNA-sequencing. PLoS Pathog. 6:e1000834.
5. Choi, A.H., Slamti, L., Avci, F.Y., Pier, G.B., and Maira-Litŕan, T. 2009. The pgaABCD locus of Acinetobacter baumannii encodes the production of poly-beta-1-6-N-acetylglucosamine, which is critical for biofilm formation. J. Bacteriol 191:5953-5963.
6. Dorsey, C.W., Tomaras, A.P., and Actis, L.A. 2002 Genetic and phenotypic analysis of Acinetobacter baumannii insertion derivatives generated with a transposome system. Appl. Environ. Microbiol 68:6353-6360.
7. Hujer, K.M., Hujer, A.M., Hulten, E.A., Bajaksouzian, S., Adams, J.M., Donskey, C.J., Ecker, D.J., Massire, C., Eshoo, M.W., Sampath, R., Thomson, J.M., Rather, P.N., Craft, D.W., Fishbain, J.T., Ewell, A.J., Jacobs, M.R., Paterson, D.L., and Bonomo, R.A. 2006. Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter sp. isolates from military and civilian patients treated at theWalter Reed Army Medical Center. Antimicrob. Agents Chemother 50:4114-4123.
8. Hunger, M., Schmucker, R., Kishan, V., and Hillen, W. 1990. Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids. Gene 87:45-51.
9. Jacobs, A.C., Thompson, M.G., Black, C.C., Kessler, J.L., Clark, L.P., McQueary, C.N., Gancz, H.Y., Corey, B.W., Moon, J.K., Si, Y., Owen, M.T., Hallock, J.D., Kwak, Y.I., Summers, A., Li, C.Z., Rasko, D.A., Penwell, W.F., Honnold, C.L., Wise, M.C., Waterman, P.E., Lesho, E.P., Stewart, R.L., Actis, L.A., Palys, T.J., Craft, D.W., and Zurawski, D.V. 2014 AB5075, a highly virulent isolate of Acinetobacter baumannii, as a model strain for the evaluation of pathogenesis and antimicrobial treatments MBio 5:e01076-14.
10. Kalivoda, E.J., Horzempa, J., Stella, N.A., Sadaf, A., Kowalski, R.P., Nau, G.J., and Shanks, R.M. 2011. New vector tools with a hygromycin resistance marker for use with opportunistic pathogens. Mol. Biotechnol. 48:7-14.
11. Kumar, A., Dalton, C., Cortez-Cordova, J., and Schweizer, H.P. 2010. Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii. J. Microbiol. Methods 82:296-300.
12. Luke, N.R., Sauberan, S.L., Russo, T.A., Beanan, J.M., Olson, R., Loehfelm, T.W., Cox, A.D., St Michael, F., Vinogradov, E.V., and Campagnari, A.A. 2010. Identification and characterization of a glycosyltransferase involved in Acinetobacter baumannii lipopolysaccharide core biosynthesis Infect. Immun. 78:2017-2023.
13. McConnell, M.J., Actis, L., and Pachón, J. 2013 Acinetobacter baumannii: Human infections, factors contributing to pathogenesis and animal models. FEMS Microbiol. Rev. 37:130-155.
14. McQueary, C.N., Kirkup, B.C., Si, Y., Barlow, M., Actis, L.A., Craft, D.W., and Zurawski, D.V. 2012. Extracellular stress and lipopolysaccharide modulate Acinetobacter baumannii surfaceassociated motility. J. Microbiol. 50:434-443.
15. Mussi, M.A., Gaddy, J.A., Cabruja, M., Arivett, B.A., Viale, A.M., Rasia, R., and Actis, L.A. 2010. The opportunistic human pathogen Acinetobacter baumannii senses and responds to light J. Bacteriol. 192:6336-6345.
16. O'Shea, M.K. 2012. Acinetobacter in modern warfare Int. J. Antimicrob. Agents 39:363-375.
17. Quandt, J. and Hynes, M.F. 1993. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127:15-21.
18. Roca, I., Marti, S., Espinal, P., Martínez, P., Gibert, I., and Vila, J. 2009. CraA, a major facilitator superfamily efflux pump associated with chloramphenicol resistance in Acinetobacter baumannii Antimicrob. Agents Chemother. 53:4013-4014.
19. Schweizer, H.P. and Hoang, T.T. 1995. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa Gene 158:15-22.
20. Tucker, A.T., Nowicki, E.M., Boll, J.M., Knauf, G.A., Burdis, N.C., Trent, M.S., and Davies, B.W. 2014. Defining gene-phenotype relationships in Acinetobacter baumannii through one-step chromosomal gene inactivation. mBio 5:e01313-14.
21. Weber, B.S., Miyata, S.T., Iwashkiw, J.A., Mortensen, B.L., Skaar, E.P., Pukatzki, S., and Feldman, M.F. 2013. Genomic and functional analysis of the type VI secretion system in Acinetobacter. PLoS One 8:e55142.
22. Wilharm, G., Piesker, J., Laue, M., and Skiebe, E 2013. DNA uptake by the nosocomial pathogen Acinetobacter baumannii occurs during movement along wet surfaces. J. Bacteriol. 195:4146-4153.
23. Zimbler, D.L., Arivett, B.A., Beckett, A.C., Menke, S.M., and Actis, L.A. 2013. Functional features of TonB energy transduction systems of Acinetobacter baumannii. Infect. Immun. 81:3382-3394.