Mini-Tn7 vectors as genetic tools for single copy gene cloning in Acinetobacter baumannii

Journal of Microbiological Methods

Volumn 82, Issue 3, 2010, Pages 296-300

  Kumar, Ayush ^a   Dalton, Courtney ^a   Cortez Cordova, Jenny ^a   Schweizer, Herbert P ^b  

^a UNIVERSITY OF ONTARIO INSTITUTE OF TECHNOLOGY   (Canada)

^b Department of Ecosystem Science and Sustainability   (United States)

Author keywords

A. baumannii; Mini Tn7; Single copy gene cloning

Indexed keywords

BACTERIAL VECTOR; GLUTAMINE FRUCTOSE 6 PHOSPHATE AMINOTRANSFERASE; GREEN FLUORESCENT PROTEIN; PLASMID VECTOR;

ACINETOBACTER BAUMANNII; ARTICLE; BACTERIAL GENOME; BACTERIAL STRAIN; CHROMOSOME INSERTION; CLONING VECTOR; CONTROLLED STUDY; GENE DOSAGE; GENE EXPRESSION PROFILING; GENE INSERTION SEQUENCE; GENE LOCATION; GENETIC COMPLEMENTATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; TRANSPOSON; VALIDATION PROCESS;

ACINETOBACTER BAUMANNII; NEGIBACTERIA;

EID: 77955660026     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.07.002     Document Type: Article

References (19)

1. Beck-Sague C.M., Jarvis W.R. Epidemic bloodstream infections associated with pressure transducers: a persistent problem. Infect. Control Hosp. Epidemiol. 1989, 10:54-59.
2. Bou G., Cervero G., Dominguez M.A., Quereda C., Martinez-Beltran J. Characterization of a nosocomial outbreak caused by a multiresistant Acinetobacter baumannii strain with a carbapenem-hydrolyzing enzyme: high-level carbapenem resistance in A. baumannii is not due solely to the presence of beta-lactamases. J. Clin. Microbiol. 2000, 38:3299-3305.
3. Boyer H.W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 1969, 41:459-472.
4. Choi K.H., Schweizer H.P. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 2006, 1:153-161.
5. Choi K.H., Schweizer H.P. mini-Tn7 insertion in bacteria with secondary, non-glmS-linked attTn7 sites: example Proteus mirabilis HI4320. Nat. Protoc. 2006, 1:170-178.
6. Choi K.H., Gaynor J.B., White K.G., Lopez C., Bosio C.M., Karkhoff-Schweizer R.R., Schweizer H.P. A Tn7-based broad-range bacterial cloning and expression system. Nat. Methods. 2005, 2:443-448.
7. Choi K.H., DeShazer D., Schweizer H.P. mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344. Nat. Protoc. 2006, 1:162-169.
8. Clinton K.M., Duane R.H. Acinetobacter Infection in the ICU. Crit. Care Clinics. 2008, 24:237.
9. Davis K.A., Moran K.A., McAllister C.K., Gray P.J. Multidrug-resistant Acinetobacter extremity infections in soldiers. Emerg. Infect. Dis. 2005, 11:1218-1224.
10. Elston J.W., Bannan C.L., Chih D.T., Boutlis C.S. Acinetobacter spp. in gunshot injuries. Emerg. Infect. Dis. 2008, 14:178-180.
11. Figurski D.H., Helinski D.R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. U.S.A. 1979, 76:1648-1652.
12. Gillis R.J., White K.G., Choi K.H., Wagner V.E., Schweizer H.P., Iglewski B.H. Molecular basis of azithromycin-resistant Pseudomonas aeruginosa biofilms. Antimicrob. Agents Chemother. 2005, 49:3858-3867.
13. Herrero M., De Lorenzo V., Timmis K.N. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria. J. Bacteriol. 1990, 172:6557-6567.
14. Hoang T.T., Karkhoff-Schweizer R.R., Kutchma A.J., Schweizer H.P. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 1998, 212:77-86.
15. Jittawuttipoka T., Buranajitpakorn S., Fuangthong M., Schweizer H.P., Vattanaviboon P., Mongkolsuk S. Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp. FEMS Microbiol. Lett. 2009, 298:111-117.
16. Kumar A., Chua K.L., Schweizer H.P. Method for regulated expression of single-copy efflux pump genes in a surrogate Pseudomonas aeruginosa strain: identification of the BpeEF-OprC chloramphenicol and trimethoprim efflux pump of Burkholderia pseudomallei 1026b. Antimicrob. Agents Chemother. 2006, 50:3460-3463.
17. Mima T., Joshi S., Gomez-Escalada M., Schweizer H.P. Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins. J. Bacteriol. 2007, 189:7600-7609.
18. Peters J.E., Craig N.L. Tn7: smarter than we thought. Nat. Rev. Mol. Cell Biol. 2001, 2:806-814.
19. Waddell C.S., Craig N.L. Tn7 transposition: recognition of the attTn7 target sequence. Proc. Natl Acad. Sci. USA 1989, 86:3958-3962.