High efficiency electrotransformation of Lactobacillus casei

FEMS Microbiology Letters

Volumn 362, Issue 2, 2015, Pages

  Welker, Dennis L ^a   Hughes, Joanne E ^a   Steele, James L ^b   Broadbent, JeffR ^a  

^a UTAH STATE UNIVERSITY   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Electroporation; Genetic manipulation; Lactic acid bacteria; Transformation

Indexed keywords

BACTERIAL DNA; DITHIOTHREITOL; GLYCINE; LITHIUM ACETATE; SODIUM CHLORIDE; WATER; ACETIC ACID DERIVATIVE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; COLONY FORMING UNIT; CONTROLLED STUDY; ELECTROTRANSFORMATION; GENE INACTIVATION; GENETIC MANIPULATION; GROWTH INHIBITION; LACTIC ACID BACTERIUM; LACTOBACILLUS CASEI; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; BACTERIUM TRANSFORMATION; ELECTROPORATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; PHYSIOLOGY; PROCEDURES;

ACETATES; DNA, BACTERIAL; ELECTROPORATION; GLYCINE; LACTOBACILLUS CASEI; SODIUM CHLORIDE; TRANSFORMATION, BACTERIAL;

EID: 84949216054     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1093/femsle/fnu033     Document Type: Article

References (17)

1. Broadbent JR, Neeno-Eckwall EC, Stahl B, et al. Analysis of the Lactobacillus casei supragenome and its influence in species evolution and lifestyle adaptation. BMC Genomics 2012;13:533.
2. Broadbent JR, Oberg TS, Hughes JE, et al. Influence of polysorbate 80 and cyclopropane fatty acid synthase activity on the lactic acid production by Lactobacillus casei ATCC 334 at lowpH. J Ind Microbiol Biot 2014;41:545-53.
3. Chassy BM, Flickinger JL. Transformation of Lactobacillus casei by electroporation. FEMS Microbiol Lett 1987;44:173-7.
4. Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 1987;19:11-5.
5. Holo H, Nes IF. High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl Environ Microb 1989;55:3119-23.
6. Kondo JK, Johansen E. Product development strategies for foods in the era of molecular biotechnology. Anton Leeuw 2002;82:291-302.
7. Luchansky JB, Muriana PM, Klaenhammer TR. Application of electroporation for the transfer of plasmid DNA to Lactobacillus, Lactococcus, Leuconostoc, Listera, Pediococcus, Bacillus, Staphylococcus, Enterococcus and Propionibacterium. Mol Microbiol 1988;2:637-46.
8. Mason CK, Collins MA, Thompson K. Modified electroporation protocol for Lactobacilli isolated from the chicken crop facilitates transformation and the use of a genetic tool. J Microbiol Meth 2005;60:353-63.
9. Natori Y, Kano Y, Imamoto F. Genetic transformation of Lactobacillus casei by electroporation. Biochimie 1990;72:265-9.
10. O'Sullivan DJ, Klaenhammer TR. High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening. Gene 1993;137:227-31.
11. Palomino MM, Allievi MC, Gründling A, et al. Osmotic stress adaptation in Lactobacillus casei BL23 leads to structural changes in the cell wall polymer lipoteichoic acid. Microbiology 2013;159:2416-26.
12. Palomino MM, Allievi MC, Prado-Acosta M, et al. New method for electroporation of Lactobacillus species grown in high salt. J Microbiol Meth 2010;83:164-7.
13. Papagianni M, Avramidis N, Filioussis G. High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol. BMC Biotechnol 2007;7:15.
14. Serafini F, Turroni F, Guglielmetti S, et al. An efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria. FEMS Microbiol Lett 2012;333:146-52.
15. Spath K, Heinl S, Egger E, et al. Lactobacillus plantarum and Lactobacillus buchneri as expression systems: evaluation of different origins of replication for the design of suitable shuttle vectors. Mol Biotechnol 2012;52:40-8.
16. Spath K, Heinl S, Grabherr R. Direct cloning in Lactobacillus plantarum: electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete. Microb Cell Fact 2012;11:141.
17. Wei M-Q, Rush CM, Norman JM, et al. An improved method for transformation of Lactobacillus strains using electroporation. J Microbiol Meth 1995;21:97-109.