Sedimentation Equilibrium Analysis of ClpB Self-Association in Diluted and Crowded Solutions

Methods in Enzymology

Volumn 562, Issue , 2015, Pages 135-160

  Alfonso, Carlos ^a   Del Castillo, Urko ^b   Martín, Ianire ^b   Muga, Arturo ^b   Rivas, Germán ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b CSIC UPV Unidad de Biofisica UBF   (Spain)

Author keywords

Analytical ultracentrifugation; Chaperone; ClpB; Macromolecular crowding; Protein aggregation; Protein protein interactions; Static light scattering

Indexed keywords

HEAT SHOCK PROTEIN; NUCLEOTIDE; OLIGOMER; PROTEIN AGGREGATE; PROTEIN CLPB; PROTEIN DISAGGREGASE; UNCLASSIFIED DRUG; ADENOSINE TRIPHOSPHATE; CLPB PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; POTASSIUM CHLORIDE; SOLUTION AND SOLUBILITY;

ENVIRONMENTAL FACTOR; ENZYME ACTIVITY; ENZYME STABILITY; HYDRODYNAMICS; IONIC STRENGTH; LIGHT SCATTERING; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN FUNCTION; SEDIMENTATION; SEDIMENTATION EQUILIBRIUM; SEDIMENTATION RATE; ULTRACENTRIFUGATION; CHEMISTRY; ISOLATION AND PURIFICATION; OSMOLARITY; PROTEIN MULTIMERIZATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; SOLUTION AND SOLUBILITY;

ADENOSINE TRIPHOSPHATE; ESCHERICHIA COLI PROTEINS; HEAT-SHOCK PROTEINS; HYDRODYNAMICS; OSMOLAR CONCENTRATION; POTASSIUM CHLORIDE; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; SOLUTIONS; ULTRACENTRIFUGATION;

EID: 84948582332     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2015.04.007     Document Type: Chapter

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