One-step purification and immobilization of his-tagged protein via Ni2+-functionalized Fe3O4@polydopamine magnetic nanoparticles

Biotechnology and Bioprocess Engineering

Volumn 20, Issue 5, 2015, Pages 901-907

  Yang, Jianbing ^a   Ni, Kefeng ^a   Wei, Dongzhi ^a   Ren, Yuhong ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

His tagged protein; immobilization; magnetic nanoparticles; polydopamine; purification

Indexed keywords

ENZYME ACTIVITY; ENZYMES; MAGNETISM; NANOPARTICLES; NICKEL; PROTEINS; PURIFICATION; RADIOACTIVE WASTE VITRIFICATION; REUSABILITY; SYNTHESIS (CHEMICAL);

AFFINITY INTERACTIONS; HIS-TAGGED PROTEINS; IMMOBILIZED ENZYME; MAGNETIC NANO-PARTICLES; OMEGA-TRANSAMINASES; ONE-STEP PURIFICATION AND IMMOBILIZATIONS; POLYDOPAMINE; RED FLUORESCENT PROTEINS;

NANOMAGNETICS;

AMINOTRANSFERASE; DOPAMINE; HIS TAGGED PROTEIN; IMMOBILIZED ENZYME; IRON OXIDE; MAGNETIC NANOPARTICLE; NANOCOATING; NICKEL; POLYDOPAMINE MAGNETIC NANOPARTICLE; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONJUGATION; CONTROLLED STUDY; ENZYME STABILITY; PROTEIN IMMOBILIZATION; PROTEIN PURIFICATION;

EID: 84947944882     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-015-0136-7     Document Type: Article

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