Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus

Journal of Biomedical Optics

Volumn 20, Issue 10, 2015, Pages

  Yamashita, Norio ^a   Morita, Masahiko ^a   Legant, Wesley R ^b   Chen, Bi Chang ^b,c   Betzig, Eric ^b   Yokota, Hideo ^a   Mimori Kiyosue, Yuko ^d  

^a Institute of Physical and Chemical Research   (Japan)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c RESEARCH CENTER FOR APPLIED SCIENCES   (Taiwan)

^d RIKEN   (Japan)

Author keywords

automated three dimensional tracking; EB1 GFP; lattice light sheet microscopy; mathematical computing; microtubules; mitosis

Indexed keywords

CELL PROLIFERATION; MACHINERY; PROTEINS;

EB1-GFP; LIGHT-SHEET MICROSCOPIES; MATHEMATICAL COMPUTING; MICROTUBULES; MITOSIS; THREE-DIMENSIONAL TRACKING;

FILAMENTS (LAMP);

FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MAPRE1 PROTEIN, HUMAN; MICROTUBULE ASSOCIATED PROTEIN;

CELL ENLARGEMENT; CELL TRACKING; FLUORESCENCE MICROSCOPY; GENETICS; HELA CELL LINE; HUMAN; MICROTUBULE; PHYSIOLOGY; PROCEDURES; REPORTER GENE; SPINDLE APPARATUS; THREE DIMENSIONAL IMAGING; ULTRASTRUCTURE;

CELL ENLARGEMENT; CELL TRACKING; FLUORESCENT DYES; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, FLUORESCENCE; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; RECOMBINANT FUSION PROTEINS; SPINDLE APPARATUS;

EID: 84947460154     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.JBO.20.10.101206     Document Type: Article

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