Immunofluorescence tomography of mouse ocular surface epithelial stem cells and their niche microenvironment

Investigative Ophthalmology and Visual Science

Volumn 56, Issue 12, 2015, Pages 7338-7344

  Parfitt, Geraint J ^a   Kavianpour, Behdad ^a   Wu, Karen L ^a   Xie, Yilu ^a   Brown, Donald J ^a   Jester, James V ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

3 D reconstruction; Conjunctiva; Cornea; H2B GFP; Limbal epithelial stem cells

Indexed keywords

ABC TRANSPORTER B5; B LYMPHOCYTE INDUCED MATURATION PROTEIN 1; CELL MARKER; COLLAGEN TYPE 4; CYTOKERATIN 5; DOXYCYCLINE; GREEN FLUORESCENT PROTEIN; HISTONE H2B; TRANSCRIPTION FACTOR SOX9;

ANIMAL TISSUE; ARTICLE; CELL DIVISION; FLUORESCENCE MICROSCOPE; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOFLUORESCENCE TOMOGRAPHY; IMMUNOHISTOCHEMISTRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; STEM CELL NICHE; TOMOGRAPHY; ANIMAL; CELL COUNT; CELL CULTURE; CELL CYCLE; CONJUNCTIVA; CORNEA EPITHELIUM; CYTOLOGY; PROCEDURES; STEM CELL; THREE DIMENSIONAL IMAGING; TRANSGENIC MOUSE;

ANIMALS; CELL COUNT; CELL CYCLE; CELLS, CULTURED; CONJUNCTIVA; EPITHELIUM, CORNEAL; FLUORESCENT ANTIBODY TECHNIQUE; IMAGING, THREE-DIMENSIONAL; MICE; MICE, TRANSGENIC; STEM CELL NICHE; STEM CELLS; TOMOGRAPHY;

EID: 84946944997     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.15-18038     Document Type: Article

References (38)

1. Stepp MA, Zieske JD. The corneal epithelial stem cell niche. Ocul Surf. 2005;3:15-26.
2. Alonso L, Fuchs E. Stem cells of the skin epithelium. Proc Natl Acad Sci U S A. 2003;100(suppl 1):11830-11835.
3. Slack JM. Stem cells in epithelial tissues. Science. 2000;287: 1431-1433.
4. Thoft RA, Friend J. The X, Y, Z hypothesis of corneal epithelial maintenance. Invest Ophthalmol Vis Sci. 1983;24:1442-1443.
5. Li W, Hayashida Y, Chen YT, Tseng SC. Niche regulation of corneal epithelial stem cells at the limbus. Cell Res. 2007;17: 26-36.
6. Watt FM, Hogan BL. Out of Eden: stem cells and their niches. Science. 2000;287:1427-1430.
7. Lavker RM, Sun TT. Epidermal stem cells: properties, markers, and location. Proc Natl Acad Sci U S A. 2000;97:13473-13475.
8. Schermer A, Galvin S, Sun TT. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol. 1986;103:49-62.
9. Pellegrini G, Golisano O, Paterna P, et al. Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J Cell Biol. 1999;145:769-782.
10. Budak MT, Alpdogan OS, Zhou M, Lavker RM, Akinci MA, Wolosin JM. Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells. J Cell Sci. 2005;118:1715-1724.
11. Cotsarelis G, Cheng SZ, Dong G, Sun TT, Lavker RM. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell. 1989;57:201-209.
12. Zhao J, Mo V, Nagasaki T. Distribution of label-retaining cells in the limbal epithelium of a mouse eye. J Histochem Cytochem. 2009;57:177-185.
13. Kuwagata M, Ogawa T, Nagata T, Shioda S. The evaluation of early embryonic neurogenesis after exposure to the genotoxic agent 5-bromo-2′-deoxyuridine in mice. Neurotoxicology. 2007;28:780-789.
14. Duque A, Rakic P. Different effects of bromodeoxyuridine and [3H]thymidine incorporation into DNA on cell proliferation, position, and fate. J Neurosci. 2011;31:15205-15217.
15. Fuchs E, Horsley V. Ferreting out stem cells from their niches. Nat Cell Biol. 2011;13:513-518.
16. Tumbar T, Guasch G, Greco V, et al. Defining the epithelial stem cell niche in skin. Science. 2004;303:359-363.
17. Pajoohesh-Ganji A, Stepp MA. In search of markers for the stem cells of the corneal epithelium. Biol Cell. 2005;97:265- 276.
18. Chen Z, de Paiva CS, Luo L, Kretzer FL, Pflugfelder SC, Li DQ. Characterization of putative stem cell phenotype in human limbal epithelia. Stem Cells. 2004;22:355-366.
19. Schlotzer-Schrehardt U, Kruse FE. Identification and characterization of limbal stem cells. Exp Eye Res. 2005;81:247-264.
20. Turner CA Jr, Mack DH, Davis MM. Blimp-1, a novel zinc fingercontaining protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells. Cell. 1994; 77:297-306.
21. Katoh K, Omori Y, Onishi A, Sato S, Kondo M, Furukawa T. Blimp1 suppresses Chx10 expression in differentiating retinal photoreceptor precursors to ensure proper photoreceptor development. J Neurosci. 2010;30:6515-6526.
22. Horsley V, O'Carroll D, Tooze R, et al. Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland. Cell. 2006;126:597-609.
23. Wei ZG, Sun TT, Lavker RM. Rabbit conjunctival and corneal epithelial cells belong to two separate lineages. Invest Ophthalmol Vis Sci. 1996;37:523-533.
24. Wei ZG, Cotsarelis G, Sun TT, Lavker RM. Label-retaining cells are preferentially located in fornical epithelium: implications on conjunctival epithelial homeostasis. Invest Ophthalmol Vis Sci. 1995;36:236-246.
25. Wei ZG, Lin T, Sun TT, Lavker RM. Clonal analysis of the in vivo differentiation potential of keratinocytes. Invest Ophthalmol Vis Sci. 1997;38:753-761.
26. Parfitt GJ, Geyfman M, Xie Y, Jester JV. Characterization of quiescent epithelial cells in mouse meibomian glands and hair follicle/sebaceous glands by immunofluorescence tomography. J Invest Dermatol. 2015;135:1175-1177.
27. Parfitt GJ, Xie Y, Geyfman M, Brown DJ, Jester JV. Absence of ductal hyper-keratinization in mouse age-related meibomian gland dysfunction (ARMGD). Aging. 2013;5:825-834.
28. Diamond I, Owolabi T, Marco M, Lam C, Glick A. Conditional gene expression in the epidermis of transgenic mice using the tetracycline-regulated transactivators tTA and rTA linked to the keratin 5 promoter. J Invest Dermatol. 2000;115:788-794.
29. Parfitt GJ, Xie Y, Reid KM, Dervillez X, Brown DJ, Jester JV. A novel immunofluorescent computed tomography (ICT) method to localise and quantify multiple antigens in large tissue volumes at high resolution. PLoS One. 2012;7:e53245.
30. Fuchs E. The tortoise and the hair: slow-cycling cells in the stem cell race. Cell. 2009;137:811-819.
31. Di Girolamo N, Bobba S, Raviraj V, et al. Tracing the fate of limbal epithelial progenitor cells in the murine cornea. Stem Cells. 2015;33:157-169.
32. Kawakita T, Espana EM, He H, Li W, Liu CY, Tseng SC. Intrastromal invasion by limbal epithelial cells is mediated by epithelial-mesenchymal transition activated by air exposure. Am J Path. 2005;167:381-393.
33. de Paiva CS, Chen Z, Corrales RM, Pflugfelder SC, Li DQ. ABCG2 transporter identifies a population of clonogenic human limbal epithelial cells. Stem Cells. 2005;23:63-73.
34. Epstein SP, Wolosin JM, Asbell PA. P63 expression levels in side population and low light scattering ocular surface epithelial cells. Trans Am Ophthalmol Soc. 2005;103:187-199, discussion 199.
35. Nowak JA, Polak L, Pasolli HA, Fuchs E. Hair follicle stem cells are specified and function in early skin morphogenesis. Cell Stem Cell. 2008;3:33-43.
36. Wirtschafter JD, Ketcham JM, Weinstock RJ, Tabesh T, McLoon LK. Mucocutaneous junction as the major source of replacement palpebral conjunctival epithelial cells. Invest Ophthalmol Vis Sci. 1999;40:3138-3146.
37. Parfitt GJ, Geyfman M, Xie Y, Jester JV. Characterization of quiescent epithelial cells in mouse meibomian glands and hair follicle/sebaceous glands by immunofluorescence tomography. J Invest Derm. 2015;135:1175-1177.
38. Wei ZG, Wu RL, Lavker RM, Sun TT. In vitro growth and differentiation of rabbit bulbar, fornix, and palpebral conjunctival epithelia. Implications on conjunctival epithelial transdifferentiation and stem cells. Invest Ophthalmol Vis Sci. 1993;34:1814-1828.