TSG101 silencing suppresses hepatocellular carcinoma cell growth by inducing cell cycle arrest and autophagic cell death

Medical Science Monitor

Volumn 21, Issue , 2015, Pages 3371-3379

  Shao, Zhuo ^a   Ji, Weiping ^a   Liu, Anan ^b   Qin, Ancheng ^c   Shen, Li ^a   Li, Gang ^a   Zhou, Yinqi ^a   Hu, Xiangui ^a   Yu, Enda ^a   Jin, Gang ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

^b SECOND MILITARY MEDICAL UNIVERSITY   (China)

^c SUZHOU MUNICIPAL HOSPITAL   (China)

Author keywords

Autophagy; Carcinoma, Hepatocellular; Cell cycle; Genes, tumor suppressor; RNA; Small interfering

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; PROTEIN KINASE B; SMALL INTERFERING RNA; TUMOR SUSCEPTIBILITY GENE 101 PROTEIN; CYCLIN A; CYCLIN D; DNA BINDING PROTEIN; ESCRT PROTEIN; TRANSCRIPTION FACTOR;

APOPTOSIS; ARTICLE; AUTOPHAGY; CELL CYCLE ARREST; CELL CYCLE ASSAY; CELL GROWTH ASSAY; CELL INVASION ASSAY; CELL MIGRATION ASSAY; CONTROLLED STUDY; FLOW CYTOMETRY; GENE DELETION; GENE EXPRESSION; GENE OVEREXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; LIVER CELL CARCINOMA; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; TUMOR GROWTH; WESTERN BLOTTING; CARCINOMA, HEPATOCELLULAR; CELL CYCLE CHECKPOINT; CELL CYCLE G1 PHASE; CELL DEATH; CELL MOTION; CELL PROLIFERATION; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; GENETICS; LIVER TUMOR; METABOLISM; PHYSIOLOGY; TUMOR CELL LINE; TUMOR INVASION;

APOPTOSIS; AUTOPHAGY; CARCINOMA, HEPATOCELLULAR; CELL CYCLE CHECKPOINTS; CELL DEATH; CELL LINE, TUMOR; CELL MOVEMENT; CELL PROLIFERATION; CYCLIN A; CYCLIN D; DNA-BINDING PROTEINS; ENDOSOMAL SORTING COMPLEXES REQUIRED FOR TRANSPORT; FLOW CYTOMETRY; G1 PHASE; GENE DELETION; GENE SILENCING; HUMANS; LIVER NEOPLASMS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NEOPLASM INVASIVENESS; RNA, SMALL INTERFERING; TRANSCRIPTION FACTORS;

EID: 84946605556     PISSN: 12341010     EISSN: 16433750     Source Type: Journal    
DOI: 10.12659/MSM.894447     Document Type: Article

References (30)

1. Jemal A, Bray F, Center MM et al: Global cancer statistics. Cancer J Clin, 2011; 61(2): 69-90
2. Maluccio M, Covey A: Recent progress in understanding, diagnosing, and treating hepatocellular carcinoma. Cancer J Clin, 2012; 62(6): 394-99
3. Kudo M: Treatment of advanced hepatocellular carcinoma with emphasis on hepatic arterial infusion chemotherapy and molecular targeted therapy. Liver Cancer, 2012; 1(2): 62
4. Li L, Cohen SN: Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells. Cell, 1996; 85(3): 319-29
5. Ponting C, Cai Y, Bork P: The breast cancer gene product TSG101: a regulator of ubiquitination? J Mol Med, 1997; 75(7): 467-69
6. Garrus JE et al: Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell, 2001; 107(1): 55-65
7. Zhang Y, Song M, Cui ZS et al: Down-regulation of TSG101 by small interfering RNA inhibits the proliferation of breast cancer cells through the MAPK/ ERK signal pathway. Histol Histopathol, 2011; 26(1): 87
8. Watanabe M, Yanagi Y, Masuhiro Y et al: A putative tumor suppressor, TSG101, acts as a transcriptional suppressor through its coiled-coil domain. Biochem Biophys Res Commun, 1998; 245(3): 900-5
9. Wagner KU, Krempler A, Qi Y et al: Tsg101 is essential for cell growth, proliferation, and cell survival of embryonic and adult tissues. Mol Cell Biol, 2003. 23(1): 150-62
10. Ruland J, Sirard C, Elia A et al: p53 accumulation, defective cell proliferation, and early embryonic lethality in mice lacking tsg101. Proc Natl Acad Sci USA, 2001; 98(4): 1859-64
11. Young TW, Rosen DG, Mei FC et al: Up-regulation of tumor susceptibility gene 101 conveys poor prognosis through suppression of p21 expression in ovarian cancer. Clin Cancer Res, 2007; 13(13): 3848-54
12. Liu RT, Huang CC, You HL et al: Overexpression of tumor susceptibility gene TSG101 in human papillary thyroid carcinomas. Oncogene, 2002; 21(31): 4830-37
13. Koon N, Schneider-Stock R, Sarlomo-Rikala M et al: Molecular targets for tumour progression in gastrointestinal stromal tumours. Gut, 2004; 53(2): 235-40
14. Zhang M, Wang Q, Ren XJ et al: [Changing of TSG101 expression on the invasion and metastasis of gastric cancer cells]. Zhonghua Yi Xue Za Zhi, 2013; 93(16): 1219-23 [in Chinese]
15. Broniarczyk JK, Warowicka A, Kwaśniewska A et al: Expression of TSG101 protein and LSF transcription factor in HPV-positive cervical cancer cells. Oncol Lett, 2014; 7(5): 1409-13
16. Liu Z, Yang Z, Liu D et al: TSG101 and PEG10 are prognostic markers in squamous cell/adenosquamous carcinomas and adenocarcinoma of the gallbladder. Oncol Lett, 2014; 7(4): 1128-38
17. Ma XR, Edmund Sim UH, Pauline B et al: Overexpression of WNT2 and TSG101 genes in colorectal carcinoma. Trop Biomed, 2008; 25(1): 46-57
18. Zhu G, Gilchrist R, Borley N et al: Reduction of TSG101 protein has a negative impact on tumor cell growth. Int J Cancer, 2004; 109(4): 541-47
19. Bruix J, Gores GJ, Mazzaferro V: Hepatocellular carcinoma: clinical frontiers and perspectives. Gut, 2014; 63(5): 844-55
20. Williams GH, Stoeber K: The cell cycle and cancer. J Pathol, 2012; 226(2): 352-64
21. Morris CR, Stanton MJ, Manthey KC et al: A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. PloS One, 2012; 7(3): e34308
22. Hanahan D, Weinberg RA: Hallmarks of cancer: the next generation. Cell, 2011; 144(5): 646-74
23. Wu MH, Hong TM, Cheng HW et al: Galectin-1-mediated tumor invasion and metastasis, up-regulated matrix metalloproteinase expression, and reorganized actin cytoskeletons. Mol Cancer Res, 2009; 7(3): 311-18
24. Casimiro MC, Crosariol M, Loro E et al: Cyclins and cell cycle control in cancer and disease. Genes Cancer, 2012; 3(11-12): 649-57
25. Cuervo AM, Bergamini E, Brunk UT et al: Autophagy and aging: the importance of maintaining" clean" cells. Autophagy, 2005; 1(3): 131-40
26. Kroemer G, Levine B: Autophagic cell death: the story of a misnomer. Nat Rev Mol Cell Biol, 2008; 9(12): 1004-10
27. Ouyang L, Shi Z, Zhao S et al: Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis. Cell Prolif, 2012; 45(6): 487-98
28. Shen S, Kepp O, Kroemer G: The end of autophagic cell death? Autophagy, 2012; 8(1): 1-3
29. Owen KA, Meyer CB, Bouton AH, Casanova JE: Activation of focal adhesion kinase by Salmonella suppresses autophagy via an Akt/mTOR signaling pathway and promotes bacterial survival in macrophages. PLoS Pathog, 2014; 10(6): e1004159
30. Liu Y, Yang Y, Ye YC et al: Activation of ERK-p53 and ERK-mediated phosphorylation of Bcl-2 are involved in autophagic cell death induced by the c-Met iInhibitor SU11274 in human lung cancer A549 cells. J Pharmacol Sci, 2012; 118(4): 423-32