Nanobody-based electrochemical immunoassay for Bacillus thuringiensis Cry1Ab toxin by detecting the enzymatic formation of polyaniline

Microchimica Acta

Volumn 182, Issue 15-16, 2015, Pages 2451-2459

  Zhu, Min ^a,b   Li, Min ^a   Li, Guanghui ^a,b   Zhou, Zikai ^a   Liu, Hong ^d   Lei, Hongtao ^e   Shen, Yanfei ^a   Wan, Yakun ^a,b,c  

^a SOUTHEAST UNIVERSITY   (China)

^b SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

^c Jiangsu Nanobody Engineering and Research Center   (China)

^d Plexera LLC   (United States)

^e SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cry1Ab toxin; Differential pulse voltammetry; Immunosensor; Nanobody; Polyaniline; Sandwich immunoassay

Indexed keywords

EID: 84944512725     PISSN: 00263672     EISSN: 14365073     Source Type: Journal    
DOI: 10.1007/s00604-015-1602-9     Document Type: Article

References (35)

1. Soberon M, Gill SS, Bravo A (2009) Signaling versus punching hole: how do bacillus thuringiensis toxins kill insect midgut cells? Cell Mol Life Sci: CMLS 66(8):1337–1349. doi:10.1007/s00018-008-8330-9
2. Raybould A, Caron-Lormier G, Bohan DA (2011) Derivation and interpretation of hazard quotients to assess ecological risks from the cultivation of insect-resistant transgenic crops. J Agric Food Chem 59(11):5877–5885. doi:10.1021/jf1042079
3. Lemaux PG (2008) Genetically engineered plants and foods: a scientist's analysis of the issues (part I). Annu Rev Plant Biol 59:771–812. doi:10.1146/annurev.arplant.58.032806.103840
4. James C, International Service for the Acquisition of Agri-Biotech Applications. Global status of commercialized biotech/GM crops, 2009: brief 41. ISAAA briefs, vol no 41
5. Gassmann AJ, Petzold-Maxwell JL, Keweshan RS, Dunbar MW (2011) Field-evolved resistance to Bt maize by western corn rootworm. PLoS One 6(7), e22629. doi:10.1371/journal.pone.0022629
6. Whiting SA, Strain KE, Campbell LA, Young BG, Lydy MJ (2014) A multi-year field study to evaluate the environmental fate and agronomic effects of insecticide mixtures. Sci Total Environ 497–498:534–542. doi:10.1016/j.scitotenv.2014.07.115
7. Zhang Y, Lai C, Su R, Zhang M, Xiong Y, Qing H, Deng Y (2012) Quantification of Cry1Ab in genetically modified maize leaves by liquid chromatography multiple reaction monitoring tandem mass spectrometry using 18O stable isotope dilution. Analyst 137(11):2699–2705. doi:10.1039/c2an35383k
8. Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev: MMBR 62(3):775–806
9. Crickmore N, Zeigler DR, Feitelson J, Schnepf E, Van Rie J, Lereclus D, Baum J, Dean DH (1998) Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol Mol Biol Rev: MMBR 62(3):807–813
10. Sanahuja G, Banakar R, Twyman RM, Capell T, Christou P (2011) Bacillus thuringiensis: a century of research, development and commercial applications. Plant Biotechnol J 9(3):283–300. doi:10.1111/j.1467-7652.2011.00595.x
11. De Meyer T, Muyldermans S, Depicker A (2014) Nanobody-based products as research and diagnostic tools. Trends Biotechnol 32(5):263–270. doi:10.1016/j.tibtech.2014.03.001
12. De Genst E, Silence K, Decanniere K, Conrath K, Loris R, Kinne J, Muyldermans S, Wyns L (2006) Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies. Proc Natl Acad Sci U S A 103(12):4586–4591. doi:10.1073/pnas.0505379103
13. Choi O, Lee Y, Han I, Kim H, Goo E, Kim J, Hwang I (2013) A simple and sensitive biosensor strain for detecting toxoflavin using beta-galactosidase activity. Biosens Bioelectrons 50:256–261. doi:10.1016/j.bios.2013.06.058
14. Lai G, Yan F, Wu J, Leng C, Ju H (2011) Ultrasensitive multiplexed immunoassay with electrochemical stripping analysis of silver nanoparticles catalytically deposited by gold nanoparticles and enzymatic reaction. Anal Chem 83(7):2726–2732. doi:10.1021/ac103283p
15. Muguruma H, Hoshino T, Nowaki K (2014) Electronically type-sorted carbon nanotube-based electrochemical biosensors with glucose oxidase and dehydrogenase. ACS Appl Mater Interfaces. doi:10.1021/am506758u
16. Tang N, Zheng J, Sheng Q, Zhang H, Liu R (2011) A novel H2O2 sensor based on the enzymatically induced deposition of polyaniline at a horseradish peroxide/aligned single-wall carbon nanotubes modified Au electrode. Analyst 136(4):781–786. doi:10.1039/c0an00379d
17. Lai G, Zhang H, Tamanna T, Yu A (2014) Ultrasensitive immunoassay based on electrochemical measurement of enzymatically produced polyaniline. Anal Chem 86(3):1789–1793. doi:10.1021/ac4037119
18. Sheng Q, Zheng J (2009) Bienzyme system for the biocatalyzed deposition of polyaniline templated by multiwalled carbon nanotubes: a biosensor design. Biosens Bioelectrons 24(6):1621–1628. doi:10.1016/j.bios.2008.08.029
19. Yu H, Yan F, Dai Z, Ju H (2004) A disposable amperometric immunosensor for alpha-1-fetoprotein based on enzyme-labeled antibody/chitosan-membrane-modified screen-printed carbon electrode. Anal Biochem 331(1):98–105. doi:10.1016/j.ab.2004.03.042
20. Li H, Yan J, Ou W, Liu H, Liu S, Wan Y (2015) Construction of a biotinylated cameloid-like antibody for lable-free detection of apolipoprotein B-100. Biosens Bioelectrons 64:111–118. doi:10.1016/j.bios.2014.08.060
21. Zhu M, Hu Y, Li G, Ou W, Mao P, Xin S, Wan Y (2014) Combining magnetic nanoparticle with biotinylated nanobodies for rapid and sensitive detection of influenza H3N2. Nanoscale Res Lett 9(1):528. doi:10.1186/1556-276X-9-528
22. Wang P, Li G, Yan J, Hu Y, Zhang C, Liu X, Wan Y (2014) Bactrian camel nanobody-based immunoassay for specific and sensitive detection of Cry1Fa toxin. Toxicon: Off J Int Soc Toxinol 92:186–192. doi:10.1016/j.toxicon.2014.10.024
23. Vincke C, Gutierrez C, Wernery U, Devoogdt N, Hassanzadeh-Ghassabeh G, Muyldermans S (2012) Generation of single domain antibody fragments derived from camelids and generation of manifold constructs. Methods Mol Biol 907:145–176. doi:10.1007/978-1-61779-974-7_8
24. Mu B, Huang X, Bu P, Zhuang J, Cheng Z, Feng J, Yang D, Dong C, Zhang J, Yan X (2010) Influenza virus detection with pentabody-activated nanoparticles. J Virol Methods 169(2):282–289. doi:10.1016/j.jviromet.2010.07.024
25. Zhang X, Liu Y, Zhang C, Wang Y, Xu C, Liu X (2012) Rapid isolation of single-chain antibodies from a human synthetic phage display library for detection of Bacillus thuringiensis (Bt) Cry1B toxin. Ecotoxicol Environ Saf 81:84–90. doi:10.1016/j.ecoenv.2012.04.021
26. Zhu M, Gong X, Hu Y, Ou W, Wan Y (2014) Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1. J Transl Med 12(1):352. doi:10.1186/s12967-014-0352-5
27. Peng Y, Yi G, Gao Z (2010) A highly sensitive microRNA biosensor based on ruthenium oxide nanoparticle-initiated polymerization of aniline. Chem Commun 46(48):9131–9133. doi:10.1039/c0cc01990a
28. Liu Y, Liu Y, Feng H, Wu Y, Joshi L, Zeng X, Li J (2012) Layer-by-layer assembly of chemical reduced graphene and carbon nanotubes for sensitive electrochemical immunoassay. Biosens Bioelectrons 35(1):63–68. doi:10.1016/j.bios.2012.02.007
29. Puttharugsa C, Wangkam T, Huangkamhang N, Gajanandana O, Himananto O, Sutapun B, Amarit R, Somboonkaew A, Srikhirin T (2011) Development of surface plasmon resonance imaging for detection of Acidovorax avenae subsp. citrulli (Aac) using specific monoclonal antibody. Biosens Bioelectrons 26(5):2341–2346. doi:10.1016/j.bios.2010.10.007
30. Wang JM, Chen XP, Liang YY, Zhu HJ, Ding JT, Peng YF (2014) Influence of transgenic rice expressing a fused Cry1Ab/1Ac protein on frogs in paddy fields. Ecotoxicology 23(9):1619–1628. doi:10.1007/s10646-014-1301-z
31. Adel-Patient K, Guimaraes VD, Paris A, Drumare MF, Ah-Leung S, Lamourette P, Nevers MC, Canlet C, Molina J, Bernard H, Creminon C, Wal JM (2011) Immunological and metabolomic impacts of administration of Cry1Ab protein and MON 810 maize in mouse. PLoS One 6(1), e16346. doi:10.1371/journal.pone.0016346
32. Ming H, Wang M, Yin H (2014) Detection of Bacillus thuringiensis Cry1Ab protein based on surface plasmon resonance immunosensor. Anal Biochem 468C:59–65. doi:10.1016/j.ab.2014.09.014
33. Paul V, Steinke K, Meyer HH (2008) Development and validation of a sensitive enzyme immunoassay for surveillance of Cry1Ab toxin in bovine blood plasma of cows fed Bt-maize (MON810). Anal Chim Acta 607(1):106–113. doi:10.1016/j.aca.2007.11.022
34. Sims SR, Holden LR (1996) Insect bioassay for determining soil degradation of Bacillus thuringiensis subsp kurstaki CryIA(b) protein in corn tissue. Environ Entomol 25(3):659–664
35. Chen C, Wu J (2012) A fast and sensitive quantitative lateral flow immunoassay for Cry1Ab based on a novel signal amplification conjugate. Sensors 12(9):11684–11696. doi:10.3390/s120911684