Bactrian camel nanobody-based immunoassay for specific and sensitive detection of Cry1Fa toxin

Toxicon

Volumn 92, Issue , 2014, Pages 186-192

  Wang, Pingyan ^a   Li, Guanghui ^a   Yan, Junrong ^a   Hu, Yonghong ^c   Zhang, Cunzheng ^d   Liu, Xianjin ^d   Wan, Yakun ^a,b  

^a SOUTHEAST UNIVERSITY   (China)

^b Jiangsu Nanobody Engineering and Research Center   (China)

^c NANJING UNIVERSITY   (China)

^d INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Biotin; Cry1Fa; Nanobody; Phage displayed library; Sandwich immune ELISA; Streptavidin

Indexed keywords

BIOTIN; CRY1FA TOXIN; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN HEAVY CHAIN; NANOBODY; TOXIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; ENDOTOXIN; FREUND ADJUVANT; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; PEPTIDE LIBRARY;

ANIMAL CELL; ANTIBODY AFFINITY; ARTICLE; BACTRIAN CAMEL; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOASSAY; INCUBATION TIME; LIMIT OF DETECTION; MAIZE; NONHUMAN; PHAGE DISPLAY; SANDWICH IMMUNE ELISA; SENSITIVITY AND SPECIFICITY; SOYBEAN; THERMOSTABILITY; TOXIN ANALYSIS; ANIMAL; CAMEL; DIAGNOSTIC USE; EPITOPE MAPPING; ESCHERICHIA COLI; EVALUATION STUDY; IMMUNOLOGY; ISOLATION AND PURIFICATION; PEPTIDE LIBRARY; PROCEDURES;

CAMELUS BACTRIANUS;

ANIMALS; BACTERIAL PROTEINS; CAMELS; ENDOTOXINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; ESCHERICHIA COLI; FREUND'S ADJUVANT; HEMOLYSIN PROTEINS; IMMUNOASSAY; PEPTIDE LIBRARY; SINGLE-DOMAIN ANTIBODIES;

EID: 84910040831     PISSN: 00410101     EISSN: 18793150     Source Type: Journal    
DOI: 10.1016/j.toxicon.2014.10.024     Document Type: Article

References (19)

1. Asensio, L., Gonzalez, I., Pavon, M.A., Garcia, T., Martin, R., 2008. An indirect ELISA and a PCR technique for the detection of grouper (Epinephelus marginatus) mislabeling. Food Addit. Contam. Part A, Chem. Analysis, Control, Expo. Risk Assess. 25, 677-683.
2. Bravo, A., Soberon, M., 2008. How to cope with insect resistance to Bt toxins? Trends Biotechnol. 26, 573-579.
3. Conrath, K.E., Lauwereys, M., Galleni, M., Matagne, A., Frere, J.M., Kinne, J., Wyns, L., Muyldermans, S., 2001. Beta-lactamase inhibitors derived from single-domain antibody fragments elicited in the camelidae. Antimicrob. Agents Chemother. 45, 2807-2812.
4. Culouscou, J.M., Plowman, G.D., Carlton, G.W., Green, J.M., Shoyab, M., 1993. Characterization of a breast cancer cell differentiation factor that specifically activates the HER4/p180erbB4 receptor. J. Biol. Chem. 268, 18407-18410.
5. Dale, P.J., Clarke, B., Fontes, E.M., 2002. Potential for the environmental impact of transgenic crops. Nat. Biotechnol. 20, 567-574.
6. He, T., Wang, Y., Li, P., Zhang, Q., Lei, J., Zhang, Z., Ding, X., Zhou, H., Zhang, W., 2014. A nanobody-based enzyme immunoassay for aflatoxin in agro-products with high tolerance to co-solvent methanol. Anal. Chem..
7. Hmila, I., Abdallah, R.B., Saerens, D., Benlasfar, Z., Conrath, K., Ayeb, M.E., Muyldermans, S., Bouhaouala-Zahar, B., 2008. VHH, bivalent domains and chimeric heavy chain-only antibodies with high neutralizing efficacy for scorpion toxin AahI'. Mol. Immunol. 45, 3847-3856.
8. Li, H., Sun, Y., Elseviers, J., Muyldermans, S., Liu, S., Wan, Y., 2014. A nanobody-based electrochemiluminescent immunosensor for sensitive detection of human procalcitonin. Analyst 139, 3718-3721.
9. Ma, L., Sun, Y., Kang, X., Wan, Y., 2014. Development of nanobody-based flow injection chemiluminescence immunoassay for sensitive detection of human prealbumin. Biosens. Bioelectron. 61, 165-171.
10. Muyldermans, S., 2013. Nanobodies: natural single-domain antibodies. Annu. Rev. Biochem. 82, 775-797.
11. Pardon, E., Laeremans, T., Triest, S., Rasmussen, S.G., Wohlkonig, A., Ruf, A., Muyldermans, S., Hol, W.G., Kobilka, B.K., Steyaert, J., 2014. A general protocol for the generation of nanobodies for structural biology. Nat. Protoc. 9, 674-693.
12. Randhawa, G.J., Singh, M., 2012. Multiplex, construct-specific, and real-time PCR-based analytical methods for Bt rice with cry1Ac gene. J. AOAC Int. 95, 186-194.
13. Romeis, J., Meissle, M., Bigler, F., 2006. Transgenic crops expressing bacillus thuringiensis toxins and biological control. Nat. Biotechnol. 24, 63-71.
14. Shelton, A.M., Zhao, J.Z., Roush, R.T., 2002. Economic, ecological, food safety, and social consequences of the deployment of bt transgenic plants. Annu. Rev. Entomol. 47, 845-881.
15. Sun, Q., Qian, J., Tian, H., Duan, L., Zhang, W., 2014. Rational design of biotinylated probes: fluorescent turn-on detection of (strept)avidin and bioimaging in cancer cells. Chem. Commun. 50, 8518-8521.
16. Tian, J.C., Wang, X.P., Long, L.P., Romeis, J., Naranjo, S.E., Hellmich, R.L., Wang, P., Earle, E.D., Shelton, A.M., 2013. Bt crops producing Cry1Ac, Cry2Ab and Cry1Fa do not harm the green lacewing, Chrysoperla rufilabris. PloS One 8, e60125.
17. Veloso, A.J., Chow, A.M., Ganesh, H.V., Li, N., Dhar, D., Wu, D.C., Mikhaylichenko, S., Brown, I.R., Kerman, K., 2014. Electrochemical immunosensors for effective evaluation of amyloid-beta modulators on oligomeric and fibrillar aggregation processes. Anal. Chem. 86, 4901-4909.
18. Vincke, C., Gutierrez, C., Wernery, U., Devoogdt, N., Hassanzadeh-Ghassabeh, G., Muyldermans, S., 2012. Generation of single domain antibody fragments derived from camelids and generation of manifold constructs. Methods Mol. Biol. 907, 145-176.
19. Zhang, X., Liu, Y., Zhang, C., Wang, Y., Xu, C., Liu, X., 2012. Rapid isolation of single-chain antibodies from a human synthetic phage display library for detection of Bacillus thuringiensis (Bt) Cry1B toxin. Ecotoxicol. Environ. Saf. 81, 84-90.