Expression, purification, and characterization of the Necator americanus aspartic protease-1 (Na-APR-1 (M74)) antigen, a component of the bivalent human hookworm vaccine

Human Vaccines and Immunotherapeutics

Volumn 11, Issue 6, 2015, Pages 1474-1488

  Seid, Christopher A ^a,b   Curti, Elena ^a,b   Jones, R Mark ^c   Hudspeth, Elissa ^a,b   Rezende, Wanderson ^a,b   Pollet, Jeroen ^a,b   Center, Lori ^a,b   Versteeg, Leroy ^a,b   Pritchard, Sonya ^c   Musiychuk, Konstantin ^c   Yusibov, Vidadi ^c   Hotez, Peter J ^a,b,d   Bottazzi, Maria Elena ^a,b,d  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b TEXAS CHILDREN S HOSPITAL   (United States)

^c Fraunhofer USA Inc   (United States)

^d BAYLOR UNIVERSITY   (United States)

Author keywords

Aspartic protease; Human hookworm; Process development; Product development partnership; Tobacco plant expression; Vaccine

Indexed keywords

ASPARTIC PROTEASE 1 ANTIGEN; ASPARTIC PROTEINASE; UNCLASSIFIED DRUG; ASPARTIC PROTEINASE-I; PARASITE ANTIGEN; RECOMBINANT VACCINE;

ANIMAL CELL; ARTICLE; CELL INFILTRATION; CHEMOLUMINESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NECATOR AMERICANUS; NECATORIASIS; NICOTIANA BENTHAMIANA; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; WESTERN BLOTTING; ANIMAL; BIOTECHNOLOGY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PHARMACEUTICS; PROCEDURES; TOBACCO; TRANSGENIC PLANT;

ANIMALS; ANTIGENS, HELMINTH; ASPARTIC ACID ENDOPEPTIDASES; BIOTECHNOLOGY; GENE EXPRESSION; NECATOR AMERICANUS; PLANTS, GENETICALLY MODIFIED; TECHNOLOGY, PHARMACEUTICAL; TOBACCO; VACCINES, SYNTHETIC;

EID: 84944457576     PISSN: 21645515     EISSN: 2164554X     Source Type: Journal    
DOI: 10.1080/21645515.2015.1036207     Document Type: Article

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