TLR5 expression in the small intestine depends on the adaptors MyD88 and TRIF, but is independent of the enteric microbiota

Gut Microbes

Volumn 6, Issue 3, 2015, Pages 202-206

  Brandão, Inês ^a   Hörmann, Nives ^a   Jäckel, Sven ^a   Reinhardt, Christoph ^a  

^a UNIVERSITY MEDICAL CENTER MAINZ   (Germany)

Author keywords

Germ free; Gut microbiota; MyD88; Small intestine; TLR2; TLR5; TOLLIP; TRIF

Indexed keywords

CASPASE 3; CYCLIN D1; FLAGELLIN; KI 67 ANTIGEN; MESSENGER RNA; MYELOID DIFFERENTIATION FACTOR 88; TOLL LIKE RECEPTOR 1; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 4; TOLL LIKE RECEPTOR 5; TOLL LIKE RECEPTOR 6; TOLL LIKE RECEPTOR ADAPTOR MOLECULE 1; MYD88 PROTEIN, HUMAN; TICAM1 PROTEIN, HUMAN; TLR5 PROTEIN, HUMAN; VESICULAR TRANSPORT ADAPTOR PROTEIN;

APOPTOSIS; ARTICLE; CELL PROLIFERATION; INNATE IMMUNITY; INTESTINE EPITHELIUM; INTESTINE FLORA; NONHUMAN; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; SMALL INTESTINE; TRANSCRIPTION REGULATION; BIOLOGICAL MODEL; BIOSYNTHESIS; GENE EXPRESSION; HUMAN; IMMUNOLOGY; METABOLISM; MICROBIOLOGY; MICROFLORA;

ADAPTOR PROTEINS, VESICULAR TRANSPORT; GASTROINTESTINAL MICROBIOME; GENE EXPRESSION; HUMANS; INTESTINE, SMALL; MICROBIOTA; MODELS, BIOLOGICAL; MYELOID DIFFERENTIATION FACTOR 88; TOLL-LIKE RECEPTOR 5;

EID: 84944446087     PISSN: 19490976     EISSN: 19490984     Source Type: Journal    
DOI: 10.1080/19490976.2015.1034417     Document Type: Article

References (21)

1. Hörmann N, Brand~ao I, Jäckel S, Ens N, Lillich M, Walter U, Reinhardt C. Gut microbial colonization orchestrates TLR2 expression, signaling and epithelial proliferation in the small intestinal mucosa. PLoS One 2014; 9(11): e113080; PMID:25396415; http://dx. doi.org/10.1371/journal.pone.0113080
2. Giraud A. Axenic mice model. Method Mol Biol 2008; 415: 321-36
3. Wooten RM, Ma Y, Yoder RA, Brown JP, Weis JH, Zachary JF, Kirschning CJ, Weis JJ. Toll-like receptor 2 is required for innate, but not acquired, host defense to Borrelia burgdorferi. J Immunol 2002; 168: 348-55; PMID:11751980; http://dx.doi. org/10.4049/ jimmunol.168.1.348
4. Reinhardt C, Bergentall M, Grainer TU, Schaffner F, Östergren-Lundén G, Petersen LC, Ruf W, Bäckhed F. Tissue factor and PAR1 promote microbiota-induced intestinal vascular remodeling. Nature 2012; 483: 627-31; PMID:22407318; http://dx.doi.org/10.1038/ nature10893
5. Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, Delzenne NM, Burcelin R. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 2008; 57: 1470-81; PMID:18305141; http://dx.doi.org/10.2337/db07-1403
6. Abreu MT. Toll-like receptor signalling in the intestinal epithelium: how bacterial recognition shapes intestinal function. Nat Rev Immunol 2010; 10: 131-44; PMID:20098461; http://dx.doi.org/10.1038/nri2707
7. Vidal K, Grosjean I, Evillard JP, Gespach C, Kaiserlian D. Immortalization of mouse intestinal epithelial cells by the SV40-large T gene. Phenotypic and immune characterization of the MODE-K cell line. J Immunol Methods 1993; 166: 63-73; PMID:7693823; http://dx. doi.org/10.1016/0022-1759(93)90329-6
8. Gewirtz AT, Navas TA, Lyons S, Godowski PJ, Madara JL. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol 2001; 167: 1882-5; PMID:11489966; http://dx.doi.org/10.4049/ jimmunol.167.4.1882
9. Stockinger S, Duerr CU, Fulde M, Dolowschiak T, Pott J, Yang I, Eibach D, Bäckhed F, Akira S, Sauerbaum S, et al. TRIF signaling drives homeostatic intestinal epithelial antimicrobial peptide expression. J Immunol 2014; 193: 4223-34; PMID:25210121; http://dx.doi.org/10.4049/jimmunol.1302708
10. Zhang G, Ghosh S. Negative regulation of toll-like receptor-mediated signaling by Tollip. J Biol Chem 2002; 277: 7059-65; PMID:11751856; http://dx.doi. org/10.1074/jbc.M109537200
11. Petnicki-Ocwieja T, Chung E, Acosta DI, Ramos LT, Shin OS, Gosh S, Kobzik L, Li X, Hu LT. TRIF mediates Toll-like receptor 2-dependent inflammatory responses to Borrelia burgdorferi. Infect Immun 2013; 81: 402-10; PMID:23166161; http://dx.doi.org/ 10.1128/IAI.00890-12
12. Choi YJ, Im E, Chung HK, Pothoulakis C, Rhee SH. TRIF mediates Toll-like receptor 5-induced signaling in intestinal epithelial cells. J Biol Chem 2010; 285: 37570-8; PMID:20855887; http://dx.doi.org/10.1074/ jbc.M110.158394
13. Sommer F, Bäckhed F. The gut microbiota-masters of host development and physiology. Nature Rev Microbiol 2013; 11: 227-38; PMID:23435359; http://dx. doi.org/10.1038/nrmicro2974
14. Melmed G, Thomas LS, Lee N, Tesfay SY, Lukasek K, Michelsen KS, Zhou Y, Hu B, Arditi M, Abreu MT. Human intestinal epithelial cells are broadly unresponsive to Toll-like receptor 2-dependent bacterial ligands: implications for host-microbial interactions in the gut. J Immunol 2003; 170: 1406-15; PMID:12538701; http://dx.doi.org/10.4049/jimmunol.170.3.1406
15. Shaykhiev R, Behr J, Bals R. Microbial patterns signaling via toll-like receptors 2 and 5 contribute to epithelial repair, growth and survival. PLoS One 2008; 3: e1393; PMID:18167552; http://dx.doi.org/10.1371/ journal.pone.0001393
16. Scheeren FA, Kuo AH, van Weele LJ, Cai S, Glykofridis I, Sikandar SS, Zabala M, Qian D, Lam JS, Johnston D, et al. A cell-intrinsic role for TLR2-MYD88 in intestinal and breast epithelia and oncogenesis. Nat Cell Biol 2014; 16: 1238-44; PMID:25362351; http:// dx.doi.org/10.1038/ncb3058
17. Rhee SH, Im E, Riegler M, Kokkotou E, O'brien M, Pothoulakis C. Pathophysiological role of toll-like receptor 5 engagement by bacterial flagellin in colonic inflammation. Proc Natl Acad Sci USA 2005; 102: 13610-5; PMID:16157881; http://dx.doi.org/10.1073/ pnas.0502174102
18. Rakoff-Nahoum S, Paglino J, Eslami-Varzaneh F, Edberg S, Medzhitov R. Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis. Cell 2004; 118: 229-41; PMID:15260992; http://dx.doi.org/10.1016/j. cell.2004.07.002
19. Vijay-Kumar M, Sanders CJ, Taylor RT, Kumar A, Aitken JD, Sitaraman SV, Neish AS, Uematsu S, Akira S, Williams IR, et al. Deletion of TLR5 results in spontaneous colitis in mice. J Clin Invest 2007; 117: 3909-21; PMID:18008007
20. Carvalho FA, Koren O, Goodrich JK, Johansson ME, Nalbantoglu I, Aitken JI, Su Y, Chassaing B, Walters WA, González A, et al. Transient inability to manage proteobacteria promotes chronic gut inflammation in TLR5-deficient mice. Cell Host Microbe 2012; 12: 139-52; PMID:22863420; http://dx.doi.org/10.1016/j. chom.2012.07.004
21. Heimesaat MM, Fischer A, Siegmund B, Kupz A, Niebergall J, Fuchs D, Jahn HK, Freudenberg M, Loddenkemper C, Batra A, et al. Shift towards proinflammatory intestinal bacteria aggravates acute murine colitis via toll-like receptors 2 and 4. PLoS One 2007; 2: e662; PMID:17653282; http://dx.doi.org/ 10.1371/journal.pone.0000662