Bioluminescent, Nonlytic, Real-Time Cell Viability Assay and Use in Inhibitor Screening

Assay and Drug Development Technologies

Volumn 13, Issue 8, 2015, Pages 456-465

  Duellman, Sarah J ^a   Zhou, Wenhui ^b   Meisenheimer, Poncho ^b   Vidugiris, Gediminas ^a   Cali, James J ^a   Gautam, Prson ^c   Wennerberg, Krister ^c   Vidugiriene, Jolanta ^a  

^a PROMEGA CORPORATION   (United States)

^b Promega Biosciences LLC   (United States)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC AGENT; BENZETHONIUM CHLORIDE; BINIMETINIB; BORTEZOMIB; CYTOSTATIC AGENT; DIGITONIN; DOXORUBICIN; IDARUBICIN; INDIBULIN; NILOTINIB; PANOBINOSTAT; SEPANTRONIUM BROMIDE; TEMSIROLIMUS; TERFENADINE; THAPSIGARGIN; TIPIFARNIB; VINCRISTINE; MOLECULAR LIBRARY;

ANIMAL CELL; ARTICLE; BIOLUMINESCENCE; CELL DEATH; CELL VIABILITY; CELL VIABILITY ASSAY; CONTROLLED STUDY; DOSE RESPONSE; DRUG EFFICACY; DRUG POTENCY; EC50; HIGH THROUGHPUT SCREENING; MAXIMUM DRUG EFFECT; MEASUREMENT; NONHUMAN; RAT; REAL TIME CELL VIABILITY ASSAY; TIME; ANALYSIS; APOPTOSIS; CELL SURVIVAL; COMPUTER SYSTEM; DRUG EFFECTS; HUMAN; K-562 CELL LINE; LUMINESCENCE; MOLECULAR LIBRARY; PHARMACOLOGY; PHYSIOLOGY; PRECLINICAL STUDY; PROCEDURES;

ANTINEOPLASTIC AGENTS; APOPTOSIS; CELL SURVIVAL; COMPUTER SYSTEMS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; HUMANS; K562 CELLS; LUMINESCENT MEASUREMENTS; SMALL MOLECULE LIBRARIES;

EID: 84944328344     PISSN: 1540658X     EISSN: 15578127     Source Type: Journal    
DOI: 10.1089/adt.2015.669     Document Type: Article

References (17)

1. Minor LK: Label-free cell-based functional assays. Comb Chem High Throughput Screen 2008;11:573-580.
2. Atienza JM, Yu N, Kirstein SL, et al.: Dynamic and label-free cell-based assays using the real-time cell electronic sensing system. Assay Drug Dev Technol 2006;4:597-607.
3. Hall MP, Unch J, Binkowski BF, et al.: Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 2012; 7:1848-1857.
4. Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65:55-63.
5. Cory AH, Owen TC, Barltrop JA, Cory JG: Use of an aqueous soluble tetrazolium/ formazan assay for cell growth assays in culture. Cancer Commun 1991;3:207-212.
6. Paull KD, Shoemaker RH, Boyd MR, Parsons JL, Risbood PA, Barbera WA: The synthesis of XTT: a new tetrazolium reagent that is bioreducible to a watersoluble formazan. J Heterocycl Chem 1988;25:911-914.
7. Ishiyama M, Shiga M, Sasamoto K, Mizoguchi M, He P: A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem Pharm Bull (Tokyo) 1993;41:1118-1122.
8. Ahmed SA, Gogal RM Jr., Walsh JE: A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J Immunol Methods 1994;170:211-224.
9. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73.
10. Pemovska T, Kontro M, Yadav B, et al.: Individualized systems medicine strategy to tailor treatments for patients with chemorefractory acute myeloid leukemia. Cancer Discov 2013;3:1416-1429.
11. Yadav B, Pemovska T, Szwajda A, et al.: Quantitative scoring of differential drug sensitivity for individually optimized anticancer therapies. Sci Rep 2014;4:5193.
12. Nakahara T, Kita A, Yamanaka K, et al.: YM155, a novel small-molecule survivin suppressant, induces regression of established human hormone-refractory prostate tumor xenografts. Cancer Res 2007;67:8014-8021.
13. Chattopadhyay S, Moran RG, Goldman ID: Pemetrexed: biochemical and cellular pharmacology, mechanisms, and clinical applications. Mol Cancer Ther 2007; 6:404-417.
14. Budzik GP, Colletti LM, Faltynek CR: Effects of methotrexate on nucleotide pools in normal human T cells and the CEM T cell line. Life Sci 2000;66:2297-2307.
15. Kaminskas E: Effects of methotrexate on ribonucleotide pools in growing and in growth-arrested tumor cells and antagonism by RNA synthesis inhibitors. J Biol Chem 1982;257:4279-4284.
16. NCI Drug Dictionary - National Cancer Institute. Available at: www.cancer.gov/ publications/dictionaries/cancer-drug (Last accessed July 23, 2015).
17. Schust J, Sperl B, Hollis A, et al.: Stattic: a small-molecule inhibitor of STAT3 activation and dimerization. Chem Biol 2006;13:1235-1242.