메뉴 건너뛰기




Volumn 13, Issue 4, 2015, Pages 485-493

Selection of suitable reference genes for normalization of genes of interest in canine soft tissue sarcomas using quantitative real-time polymerase chain reaction

Author keywords

Canine sarcoma; geNorm; GUSB; PSMB6; Reference gene; RT qPCR

Indexed keywords

BETA GLUCURONIDASE; PROTEASOME;

EID: 84944277010     PISSN: 14765810     EISSN: 14765829     Source Type: Journal    
DOI: 10.1111/vco.12108     Document Type: Article
Times cited : (10)

References (38)
  • 1
    • 27644563899 scopus 로고    scopus 로고
    • The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
    • Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, Johnson MA, et al. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Analytical Biochemistry 2005; 344: 141-143.
    • (2005) Analytical Biochemistry , vol.344 , pp. 141-143
    • Dheda, K.1    Huggett, J.F.2    Chang, J.S.3    Kim, L.U.4    Bustin, S.A.5    Johnson, M.A.6
  • 2
    • 0033775615 scopus 로고    scopus 로고
    • Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
    • Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology 2000; 25: 169-193.
    • (2000) Journal of Molecular Endocrinology , vol.25 , pp. 169-193
    • Bustin, S.A.1
  • 3
    • 77649235913 scopus 로고    scopus 로고
    • How to do successful gene expression analysis using real-time PCR
    • Derveaux S, Vandesompele J and Hellemans J. How to do successful gene expression analysis using real-time PCR. Methods 2010; 50: 227-230.
    • (2010) Methods , vol.50 , pp. 227-230
    • Derveaux, S.1    Vandesompele, J.2    Hellemans, J.3
  • 4
    • 34147198640 scopus 로고    scopus 로고
    • Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies
    • Peters IR, Peeters D, Helps CR and Day MJ. Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies. Veterinary Immunology and Immunopathology 2007; 117: 55-66.
    • (2007) Veterinary Immunology and Immunopathology , vol.117 , pp. 55-66
    • Peters, I.R.1    Peeters, D.2    Helps, C.R.3    Day, M.J.4
  • 5
    • 14044265394 scopus 로고    scopus 로고
    • Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
    • Bustin SA and Nolan T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. Journal of Biomolecular Techniques 2004; 15: 155-166.
    • (2004) Journal of Biomolecular Techniques , vol.15 , pp. 155-166
    • Bustin, S.A.1    Nolan, T.2
  • 6
    • 0036278859 scopus 로고    scopus 로고
    • Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream
    • Ginzinger DG. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Experimental Hematology 2002; 30: 503-512.
    • (2002) Experimental Hematology , vol.30 , pp. 503-512
    • Ginzinger, D.G.1
  • 7
    • 21344471138 scopus 로고    scopus 로고
    • Real-time RT-PCR normalisation; strategies and considerations
    • Huggett J, Dheda K, Bustin S and Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes and Immunity 2005; 6: 279-284.
    • (2005) Genes and Immunity , vol.6 , pp. 279-284
    • Huggett, J.1    Dheda, K.2    Bustin, S.3    Zumla, A.4
  • 8
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • research0034.1-0034.11.
    • Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002; 3: research0034.1-0034.11.
    • (2002) Genome Biology , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6
  • 9
    • 24944534978 scopus 로고    scopus 로고
    • Real-time PCR for mRNA quantitation
    • Wong ML and Medrano JF. Real-time PCR for mRNA quantitation. BioTechniques 2005; 39: 1-11.
    • (2005) BioTechniques , vol.39 , pp. 1-11
    • Wong, M.L.1    Medrano, J.F.2
  • 10
    • 3442876969 scopus 로고    scopus 로고
    • Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
    • Andersen CL, Jensen JL and Orntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Research 2004; 64: 5245-5250.
    • (2004) Cancer Research , vol.64 , pp. 5245-5250
    • Andersen, C.L.1    Jensen, J.L.2    Orntoft, T.F.3
  • 11
    • 1842483281 scopus 로고    scopus 로고
    • Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper - Excel-based tool using pair-wise correlarions
    • Pfaffl MW, Tichopad A, Prgomet C and Neuvians TP. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper - Excel-based tool using pair-wise correlarions. Biotechnology Letters 2004; 26: 509-515.
    • (2004) Biotechnology Letters , vol.26 , pp. 509-515
    • Pfaffl, M.W.1    Tichopad, A.2    Prgomet, C.3    Neuvians, T.P.4
  • 12
    • 45449087921 scopus 로고    scopus 로고
    • qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data
    • Hellemans J, Mortier G, De Paepe A, Speleman F and Vandesompele J. qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biology 2007; 8: R19.0-R19.14.
    • (2007) Genome Biology , vol.8 , pp. R190-R1914
    • Hellemans, J.1    Mortier, G.2    De Paepe, A.3    Speleman, F.4    Vandesompele, J.5
  • 13
    • 64149097786 scopus 로고    scopus 로고
    • The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
    • Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 2009; 55: 611-622.
    • (2009) Clinical Chemistry , vol.55 , pp. 611-622
    • Bustin, S.A.1    Benes, V.2    Garson, J.A.3    Hellemans, J.4    Huggett, J.5    Kubista, M.6
  • 15
    • 38549112058 scopus 로고    scopus 로고
    • Translation of new cancer treatments from pet dogs to humans
    • Paoloni M and Khanna C. Translation of new cancer treatments from pet dogs to humans. Nature Reviews. Cancer 2008; 8: 147-156.
    • (2008) Nature Reviews. Cancer , vol.8 , pp. 147-156
    • Paoloni, M.1    Khanna, C.2
  • 16
    • 0033671255 scopus 로고    scopus 로고
    • Spontaneously occurring tumors of companion animals as models for human cancer
    • Vail DM and MacEwen EG. Spontaneously occurring tumors of companion animals as models for human cancer. Cancer Investigation 2000; 18: 781-792.
    • (2000) Cancer Investigation , vol.18 , pp. 781-792
    • Vail, D.M.1    MacEwen, E.G.2
  • 18
    • 84944278962 scopus 로고    scopus 로고
    • [accessed 13 September 2012 - 21 September 2012].
    • National Center for Biotechnology Information N. 2012: http://www.ncbi.nlm.nih.gov/ [accessed 13 September 2012 - 21 September 2012].
    • (2012)
  • 19
    • 33751236463 scopus 로고    scopus 로고
    • Selection of reference genes for quantitative real-time PCR analysis in canine mammary tumors using the GeNorm algorithm
    • Etschmann B, Wilcken B, Stoevesand K, von der Schulenburg A and Sterner-Kock A. Selection of reference genes for quantitative real-time PCR analysis in canine mammary tumors using the GeNorm algorithm. Veterinary Pathology 2006; 43: 934-942.
    • (2006) Veterinary Pathology , vol.43 , pp. 934-942
    • Etschmann, B.1    Wilcken, B.2    Stoevesand, K.3    von der Schulenburg, A.4    Sterner-Kock, A.5
  • 20
    • 84865754120 scopus 로고    scopus 로고
    • Array-based comparative genomic hybridization-guided identification of reference genes for normalization of real-time quantitative polymerase chain reaction assay data for lymphomas, histiocytic sarcomas, and osteosarcomas of dogs
    • Tsai P-C and Breen M. Array-based comparative genomic hybridization-guided identification of reference genes for normalization of real-time quantitative polymerase chain reaction assay data for lymphomas, histiocytic sarcomas, and osteosarcomas of dogs. American Journal of Veterinary Research 2012; 79: 1335-1343.
    • (2012) American Journal of Veterinary Research , vol.79 , pp. 1335-1343
    • Tsai, P.-C.1    Breen, M.2
  • 23
    • 84862228981 scopus 로고    scopus 로고
    • 64Cu-ATSM and 18FDG PET uptake and 64Cu- ATSM autoradiography in spontaneous canine tumors: comparison with pimonidazole hypoxia immunohistochemistry
    • Hansen AE, Kristensen AT, Jorgensen JT, McEvoy FJ, Busk M, van der Kogel AJ, et al. 64Cu-ATSM and 18FDG PET uptake and 64Cu- ATSM autoradiography in spontaneous canine tumors: comparison with pimonidazole hypoxia immunohistochemistry. Radiation Oncology 2012; 7: 89.
    • (2012) Radiation Oncology , vol.7 , pp. 89
    • Hansen, A.E.1    Kristensen, A.T.2    Jorgensen, J.T.3    McEvoy, F.J.4    Busk, M.5    van der Kogel, A.J.6
  • 24
    • 84887339426 scopus 로고    scopus 로고
    • Dose escalation to high-risk sub-volumes based on non-invasive imaging of hypoxia and glycolytic activity in canine solid tumors: a feasibility study
    • Clausen MM, Hansen AE, Af Rosenschold PM, Kjaer A, Kristensen AT, McEvoy FJ, et al. Dose escalation to high-risk sub-volumes based on non-invasive imaging of hypoxia and glycolytic activity in canine solid tumors: a feasibility study. Radiation Oncology 2013; 8: 262.
    • (2013) Radiation Oncology , vol.8 , pp. 262
    • Clausen, M.M.1    Hansen, A.E.2    Af Rosenschold, P.M.3    Kjaer, A.4    Kristensen, A.T.5    McEvoy, F.J.6
  • 25
    • 33644975004 scopus 로고    scopus 로고
    • RNA integrity and the effect on the real-time qRT-PCR performance
    • Fleige S and Pfaffl MW. RNA integrity and the effect on the real-time qRT-PCR performance. Molecular Aspects of Medicine 2006; 27: 126-139.
    • (2006) Molecular Aspects of Medicine , vol.27 , pp. 126-139
    • Fleige, S.1    Pfaffl, M.W.2
  • 26
    • 7444226814 scopus 로고    scopus 로고
    • Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization
    • Haller F, Kulle B, Schwager S, Gunawan B, von Heydebreck A, Sultmann H, et al. Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization. Analytical Biochemistry 2004; 335: 1-9.
    • (2004) Analytical Biochemistry , vol.335 , pp. 1-9
    • Haller, F.1    Kulle, B.2    Schwager, S.3    Gunawan, B.4    von Heydebreck, A.5    Sultmann, H.6
  • 27
    • 84876154013 scopus 로고    scopus 로고
    • Selection of internal reference genes for normalization of quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis in the canine brain and other organs
    • Park SJ, Huh JW, Kim YH, Lee SR, Kim SH, Kim SU, et al. Selection of internal reference genes for normalization of quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis in the canine brain and other organs. Molecular Biotechnology 2012; 54: 47-57. doi:10.1007/s12033-012-9543-6.
    • (2012) Molecular Biotechnology , vol.54 , pp. 47-57
    • Park, S.J.1    Huh, J.W.2    Kim, Y.H.3    Lee, S.R.4    Kim, S.H.5    Kim, S.U.6
  • 28
    • 64849117301 scopus 로고    scopus 로고
    • Quantitative gene-expression of the tumor angiogenesis markers vascular endothelial growth factor, integrin αV and integrin β3 in human neuroendocrine tumors
    • Oxboel J, Binderup T, Knigge U and Kjaer A. Quantitative gene-expression of the tumor angiogenesis markers vascular endothelial growth factor, integrin αV and integrin β3 in human neuroendocrine tumors. Oncology Reports 2009; 21: 769-775.
    • (2009) Oncology Reports , vol.21 , pp. 769-775
    • Oxboel, J.1    Binderup, T.2    Knigge, U.3    Kjaer, A.4
  • 29
    • 84874116867 scopus 로고    scopus 로고
    • (64)Cu-NODAGA-c(RGDyK) is a promising new angiogenesis PET tracer: correlation between tumor uptake and integrin alpha(V)beta(3) expression in human neuroendocrine tumor xenografts
    • Oxboel J, Schjoeth-Eskesen C, El-Ali HH, Madsen J and Kjaer A. (64)Cu-NODAGA-c(RGDyK) is a promising new angiogenesis PET tracer: correlation between tumor uptake and integrin alpha(V)beta(3) expression in human neuroendocrine tumor xenografts. International Journal of Molecular Imaging. 2012; 2012: 379807.
    • (2012) International Journal of Molecular Imaging. , vol.2012 , pp. 379807
    • Oxboel, J.1    Schjoeth-Eskesen, C.2    El-Ali, H.H.3    Madsen, J.4    Kjaer, A.5
  • 30
    • 84875257999 scopus 로고    scopus 로고
    • High tumor uptake of (64)Cu: implications for molecular imaging of tumor characteristics with copper-based PET tracers
    • Jorgensen JT, Persson M, Madsen J and Kjaer A. High tumor uptake of (64)Cu: implications for molecular imaging of tumor characteristics with copper-based PET tracers. Nuclear Medicine and Biology 2013; 40: 345-350.
    • (2013) Nuclear Medicine and Biology , vol.40 , pp. 345-350
    • Jorgensen, J.T.1    Persson, M.2    Madsen, J.3    Kjaer, A.4
  • 31
    • 84870605153 scopus 로고    scopus 로고
    • 18F FLT PET for non-invasive assessment of tumor sensitivity to chemotherapy: studies with experimental chemotherapy TP202377 in human cancer xenografts in mice
    • Jensen MM, Erichsen KD, Björkling F, Madsen J, Jensen PB, Sehested M, et al. 18F FLT PET for non-invasive assessment of tumor sensitivity to chemotherapy: studies with experimental chemotherapy TP202377 in human cancer xenografts in mice. PloS One 2012; 7: e50618.
    • (2012) PloS One , vol.7 , pp. e50618
    • Jensen, M.M.1    Erichsen, K.D.2    Björkling, F.3    Madsen, J.4    Jensen, P.B.5    Sehested, M.6
  • 32
    • 84893438855 scopus 로고    scopus 로고
    • Imaging of treatment response to the combination of carboplatin and paclitaxel in human ovarian cancer xenograft tumors in mice using FDG and FLT PET
    • Jensen MM, Erichsen KD, Björkling F, Madsen J, Jensen PB, Sehested M, et al. Imaging of treatment response to the combination of carboplatin and paclitaxel in human ovarian cancer xenograft tumors in mice using FDG and FLT PET. PloS One 2013; 8: e85126.
    • (2013) PloS One , vol.8 , pp. e85126
    • Jensen, M.M.1    Erichsen, K.D.2    Björkling, F.3    Madsen, J.4    Jensen, P.B.5    Sehested, M.6
  • 33
    • 84876142262 scopus 로고    scopus 로고
    • 18F FDG and 18F FLT positron emission tomography imaging following treatment with belinostat in human ovary cancer xenografts in mice
    • Jensen MM, Erichsen KD, Johnbeck CB, Björkling F, Madsen J, Jensen PB, et al. 18F FDG and 18F FLT positron emission tomography imaging following treatment with belinostat in human ovary cancer xenografts in mice. BMC Cancer 2013; 13: 168.
    • (2013) BMC Cancer , vol.13 , pp. 168
    • Jensen, M.M.1    Erichsen, K.D.2    Johnbeck, C.B.3    Björkling, F.4    Madsen, J.5    Jensen, P.B.6
  • 34
    • 33747175374 scopus 로고    scopus 로고
    • Development and evaluation of canine reference genes for accurate quantification of gene expression
    • Brinkhof B, Spee B, Rothuizen J and Penning LC. Development and evaluation of canine reference genes for accurate quantification of gene expression. Analytical Biochemistry 2006; 356: 36-43.
    • (2006) Analytical Biochemistry , vol.356 , pp. 36-43
    • Brinkhof, B.1    Spee, B.2    Rothuizen, J.3    Penning, L.C.4
  • 36
    • 62549143913 scopus 로고    scopus 로고
    • A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis
    • Schlotter YM, Veenhof EZ, Brinkhof B, Rutten VPMG, Spee B, Willemse T, et al. A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis. Veterinary Immunology and Immunopathology 2009; 129: 115-118.
    • (2009) Veterinary Immunology and Immunopathology , vol.129 , pp. 115-118
    • Schlotter, Y.M.1    Veenhof, E.Z.2    Brinkhof, B.3    Rutten, V.P.M.G.4    Spee, B.5    Willemse, T.6
  • 37
    • 34250673544 scopus 로고    scopus 로고
    • Expression stability of commonly used reference genes in canine articular connective tissues
    • Ayers D, Clements DN, Salway F and Day PJ. Expression stability of commonly used reference genes in canine articular connective tissues. BMC Veterinary Research 2007; 3: 7.
    • (2007) BMC Veterinary Research , vol.3 , pp. 7
    • Ayers, D.1    Clements, D.N.2    Salway, F.3    Day, P.J.4
  • 38
    • 34548743038 scopus 로고    scopus 로고
    • Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data
    • Maccoux LJ, Clements DN, Salway F and Day PJ. Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data. BMC Molecular Biology 2007; 8: 62.
    • (2007) BMC Molecular Biology , vol.8 , pp. 62
    • Maccoux, L.J.1    Clements, D.N.2    Salway, F.3    Day, P.J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.