Evaluation of different phospho-tyrosine antibodies for label-free phosphoproteomics

Journal of Proteomics

Volumn 127, Issue , 2015, Pages 259-263

  van der Mijn, Johannes C ^a   Labots, Mariette ^a   Piersma, Sander R ^a   Pham, Thang V ^a   Knol, Jaco C ^a   Broxterman, Henk J ^a   Verheul, Henk M ^a   Jiménez, Connie R ^a  

^a VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Cancer; EGFR; Erlotinib; Phosphoproteomics; Signaling; Tyrosine

Indexed keywords

4G10 ANTIBODY; AGAROSE; ANTIBODY; EPHRIN RECEPTOR A2; FOCAL ADHESION KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 1; P TYR 1000 ANTIBODY; PHOSPHOPEPTIDE; PHOSPHOPROTEIN; PHOSPHOTYROSINE; PROTEIN KINASE FYN; SCATTER FACTOR RECEPTOR; UNCLASSIFIED DRUG; CANCER ANTIBODY; EGFR PROTEIN, HUMAN; EPIDERMAL GROWTH FACTOR RECEPTOR; ERLOTINIB;

ARTICLE; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETER; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEOMICS; QUANTITATIVE ANALYSIS; CHEMISTRY; EVALUATION STUDY; METABOLISM; PROCEDURES; TUMOR CELL LINE;

ANTIBODIES, NEOPLASM; CELL LINE, TUMOR; ERLOTINIB HYDROCHLORIDE; HUMANS; MASS SPECTROMETRY; PHOSPHOPROTEINS; PHOSPHOTYROSINE; RECEPTOR, EPIDERMAL GROWTH FACTOR;

EID: 84944177269     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2015.04.006     Document Type: Article

References (13)

1. Keefe D.M., Bateman E.H. Tumor control versus adverse events with targeted anticancer therapies. Nat Rev Clin Oncol Dec 20 2011, 9(2):98-109.
2. Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., et al. Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. Cell Dec 14 2007, 131(6):1190-1203.
3. Huang P.H., Mukasa A., Bonavia R., Flynn R.A., Brewer Z.E., Cavenee W.K., et al. Quantitative analysis of EGFRvIII cellular signaling networks reveals a combinatorial therapeutic strategy for glioblastoma. Proc Natl Acad Sci U S A Jul 31 2007, 104(31):12867-12872.
4. Guo A., Villen J., Kornhauser J., Lee K.A., Stokes M.P., Rikova K., et al. Signaling networks assembled by oncogenic EGFR and c-Met. Proc Natl Acad Sci U S A Jan 15 2008, 105(2):692-697.
5. 3+) affinity chromatography. Anal Biochem 1986, 154(1):250-254.
6. Villen J., Gygi S.P. The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry. Nat Protoc 2008, 3(10):1630-1638.
7. Larsen M.R., Thingholm T.E., Jensen O.N., Roepstorff P., Jorgensen T.J. Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns. Mol Cell Proteomics Jul 2005, 4(7):873-886.
8. Pinkse M.W., Uitto P.M., Hilhorst M.J., Ooms B., Heck A.J. Selective isolation at the femtomole level of phosphopeptides from proteolytic digests using 2D-NanoLC-ESI-MS/MS and titanium oxide precolumns. Anal Chem Jul 15 2004, 76(14):3935-3943.
9. Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., et al. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nat Biotechnol Jan 2005, 23(1):94-101.
10. Kettenbach A.N., Gerber S.A. Rapid and reproducible single-stage phosphopeptide enrichment of complex peptide mixtures: application to general and phosphotyrosine-specific phosphoproteomics experiments. Anal Chem Oct 15 2011, 83(20):7635-7644.
11. Sharma K., D'Souza R.C., Tyanova S., Schaab C., Wisniewski J.R., Cox J., et al. Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep Sep 11 2014, 8(5):1583-1594.
12. Vizcaino J.A., Deutsch E.W., Wang R., Csordas A., Reisinger F., Rios D., et al. ProteomeXchange provides globally coordinated proteomics data submission and dissemination. Nat Biotechnol Mar 2014, 32(3):223-226.
13. Stommel J.M., Kimmelman A.C., Ying H., Nabioullin R., Ponugoti A.H., Wiedemeyer R., et al. Coactivation of receptor tyrosine kinases affects the response of tumor cells to targeted therapies. Science Oct 12 2007, 318(5848):287-290.