Effective restoration of male-sterile (barnase) lines requires overlapping and higher levels of barstar expression: A multi-generation field analysis in Brassica juncea

Journal of Plant Biochemistry and Biotechnology

Volumn 24, Issue 4, 2015, Pages 393-399

  Bisht, Naveen C ^a,b   Jagannath, Arun ^a   Augustine, Rehna ^b   Burma, Pradeep K ^a   Gupta, Vibha ^a   Pradhan, Akshay K ^a   Pental, Deepak ^a  

^a UNIVERSITY OF DELHI   (India)

^b NATIONAL INSTITUTE OF PLANT GENOME RESEARCH   (India)

Author keywords

Barnase barstar; Brassica juncea; Expression profiles; Male fertility restoration; Male sterile plants; Transgene stability

Indexed keywords

EID: 84944069532     PISSN: 09717811     EISSN: 09741275     Source Type: Journal    
DOI: 10.1007/s13562-014-0289-z     Document Type: Article

References (13)

1. Bisht NC, Jagannath A, Burma PK, Pradhan AK, Pental D (2007) Retransformation of a male sterile barnase line with the barstar gene as an efficient alternative method to identify male sterile-restorer combinations for heterosis breeding. Plant Cell Rep 26:727–33
2. Bisht NC, Jagannath A, Gupta V, Burma PK, Pental D (2004) A two gene–two promoter system for enhanced expression of a restorer gene (barstar) and development of improved fertility restorer lines for hybrid seed production in crop plants. Mol Breed 14:129–144
3. Denis M, Delourme R, Gourret JP, Mariani C, Renard M (1993) Expression of engineered nuclear male sterility in Brassica napus: Genetics, morphology, cytology and sensitivity to temperature. Plant Physiol 101:1295–1304
4. Hartley RW, Smeaton JR (1973) On the reaction between the extracellular ribonuclease of Bacillus amyloliquifaciens (Barnase) and its intracellular inhibitor (Barstar). J Biol Chem 245:5624–5626
5. Heslop-Harrison J, Heslop-Harrison Y, Shivanna KR (1984) The evaluation of pollen quality and a further appraisal of the fluorochromatic (FCR) test procedure. Theor Appl Genet 67:367–375
6. Jagannath A, Arumugam N, Gupta V, Pradhan AK, Burma PK, Pental D (2002) Development of transgenic barstar lines and identification of a male sterile (barnase)/restorer (barstar) combination for heterosis breeding in Indian oilseed mustard (Brassica juncea). Curr Sci 82:46–52
7. Jagannath A, Bandyopadhyay P, Arumugam N, Gupta V, Burma PK, Pental D (2001) The use of a spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high frequency generation of transgenic male sterile lines in Brassica juncea L. Mol Breed 8:11–23
8. Kavita P, Burma PK (2008) A comparative analysis of green fluorescent protein and β-glucuronidase protein-encoding genes as a reporter system for studying the temporal expression profiles of promoters. J Biosci 33:337–43
9. Kempe K, Gils M (2011) Pollination control technologies for hybrid breeding. Mol Breed 27:417–437
10. Klimyuk VI, Carroll BJ, Thomas CM, Jones JDG (1993) Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J 3:493–494
11. Mariani C, De Beuckeleer M, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimeric ribonuclease gene. Nature 347:737–41
12. Mariani C, Gossele V, De Beuckeleer M, De Block M, Goldberg RB, De Greef W, Leemans J (1992) A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants. Nature 357:384–387
13. Perez-Prat E, van Lookeren Campagne MM (2002) Hybrid seed production and the challenge of propagating male-sterile plants. Trends Plant Sci 7:199–203