The use of a Spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male sterile lines in Brassica juncea L.

Molecular Breeding

Volumn 8, Issue 1, 2001, Pages 11-23

  Jagannath, Arun ^a   Bandyopadhyay, Panchali ^a   Arumugam, N ^a   Gupta, Vibha ^a   Burma, Pradeep Kumar ^a   Pental, Deepak ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

Barnase; Brassica juncea; Male sterility; Spacer DNA; Tapetum specific promoters

Indexed keywords

BACKCROSSING; BARNASE; CYTOTOXICITY; DEVELOPMENTAL STAGE; DNA FRAGMENT; GENE EXPRESSION; GENETIC TRANSFORMATION; MALE STERILITY; MARKER GENE; PHENOTYPE; PLANT BREEDING; PROMOTER REGION; SEGREGATION DISTORTION; SPACER DNA; TISSUE SPECIFICITY; TRANSGENE; TRANSGENIC PLANT;

CROPS; GENE EXPRESSION; PLANTS (BOTANY); SEED; TISSUE;

BARNASE; BRASSICA JUNCEA; CYTOTOXIC; DNA FRAGMENT; MALE STERILE; MALE STERILITY; MARKER GENES; SPACER DNA; TAPETUM-SPECIFIC PROMOTER; TISSUE-SPECIFIC EXPRESSION;

DNA;

BRASSICA; BRASSICA JUNCEA; EMBRYOPHYTA;

EID: 0034772843     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1011916216191     Document Type: Article

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