A two gene - Two promoter system for enhanced expression of a restorer gene (barstar) and development of improved fertility restorer lines for hybrid seed production in crop plants

Molecular Breeding

Volumn 14, Issue 2, 2004, Pages 129-144

  Bisht, Naveen Chandra ^a   Jagannath, Arun ^a   Gupta, Vibha ^a   Kumar Burma, Pradeep ^a   Pental, Deepak ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

Barnase barstar; Brassica juncea; Chimeric promoter; Fertility restoration; Synthetic gene; Tapetum specific promoters

Indexed keywords

CROPS; OILSEEDS; PLANTS (BOTANY); TRANSCRIPTION;

BARNASE; BARNASE-BARSTAR; BARSTAR; BRASSICA JUNCEA; CHIMERIC PROMOTERS; FERTILITY RESTORATION; MALE STERILE; SYNTHETIC GENES; TAPETUM-SPECIFIC PROMOTER; WILD TYPES;

RESTORATION;

BRASSICA; BRASSICA JUNCEA; SYNTHETIC CONSTRUCT;

EID: 4444290146     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:MOLB.0000038002.45312.08     Document Type: Article

References (22)

1. Bate N. and Twell D. 1998. Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements. Plant Mol. Biol. 37: 859-869.
2. Dillon P.J. and Rosen C.A. 1990. A rapid method for the construction of synthetic genes using the polymerase chain reaction. Biotechniques 9: 298-299.
3. Hajdukiewicz P., Svab Z. and Maliga P. 1994. The small versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol. Biol. 25: 989-994.
4. Hartley R.W. and Smeaton J.R. 1973. On the reaction between the extracellular ribonuclease of Bacillus amyloliquifaciens (Barnase) and its intracellular inhibitor (Barstar). J. Biol. Chem. 245: 5624-5626.
5. Heslop-Harrison J., Heslop-Harrison Y. and Shivanna K.R. 1984. The evaluation of pollen quality and a further appraisal of the fluorochromatic (FCR) test procedure. Theor. Appl. Genet. 67: 367-375.
6. Horton R.M. 1993. In vitro recombination and mutagenesis of DNA: SOEing together tailor-made genes. In: White B.A. (ed.), PCR Protocols : Current Methods and Applications. Humana Press, pp. 251-262.
7. Imai Y., Matsushima Y., Sugimura T. and Terada M. 1991. A simple and rapid method for generating a deletion by PCR. Nucl. Acids Res. 19: 2785.
8. Jagannath A., Bandyopadhyay P., Arumugam N., Gupta V., Burma P.K. and Pental D. 2001. The use of a Spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male sterile lines in Brassica juncea L. Mol. Breeding 8: 11-23.
9. Jagannath A., Arumugam N., Gupta V., Pradhan A.K., Burma P.K. and Pental D. 2002. Development of transgenic barstar lines and identification of a male sterile (barnase)/restorer (barstar) combination for heterosis breeding in Indian oilseed mustard (Brassica juncea). Curr. Sci. 82: 46-52.
10. Jagannath A., Bandyopadhyay P., Mehra S., Arumugam N., Burma P.K. and Pental D. 2003. Agrobacterium-mediated genetic transformation of Brassica juncea.. In: Jaiwal P.K. and Singh R.P. (eds), Plant Genetic Engineering Volume 2: Improvement of Food Crops. USA Sci Tech Publishing LLC, USA, pp. 349-360.
11. Jefferson R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387-405.
12. Klimyuk V.I., Carroll B.J., Thomas C.M. and Jones J.D.G. 1993. Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J. 3: 493-494.
13. Koltunow A.M., Truettner J., Cox K.H., Wallroth M. and Goldberg R.B. 1990. Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2: 1201-1224.
14. Mariani C., De Beuckeleer M., Truettner J., Leemans J. and Goldberg R.B. 1990. Induction of male sterility in plants by a chimeric ribonuclease gene. Nature 347: 737-741.
15. Mariani C., Gossele V., De Beuckeleer M., De Block M., Goldberg R.B., De Greef W. and Leemans J. 1992. A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants. Nature 357: 384-387.
16. Mehra S., Pareek A., Bandyopadhyay P., Sharma P., Burma P.K. and Pental D. 2000. Development of transgenics in Indian oilseed mustard (Brassica juncea) resistant to herbicide phosphinothricin. Curr Sci. 78: 1358-1364.
17. Murray E.E., Lotzer J. and Eberle M. 1989. Codon usage in plant genes. Nucl. Acids Res. 17: 477-498.
18. Nakamura Y., Gojobori T. and Ikemura T. 1997. Codon usage tabulated from the international DNA sequence databases. Nucl. Acids Res. 25: 244-245.
19. Paul W., Hodge R., Smartt S., Draper J. and Scott R. 1992. The isolation and characterization of the tapetum specific Arabidopsis thaliana A9 gene. Plant Mol. Biol. 19: 611-622.
20. nd ed. Cold Spring Harbor Laboratory Press, Plainview, New York, USA.
21. Scott R., Hodge R., Paul W. and Draper J. 1991. The molecular biology of anther differentiation. Plant Sci. 80: 167-191.
22. Wada K., Wada Y., Ishibashi F., Gojobori T. and Ikemura T. 1992. Codon usage tabulated from the GenBank genetic sequence data. Nucl. Acids Res. 20: 2111-2118.