Targeted mutagenesis in zebrafish by TALENs

Methods in Molecular Biology

Volumn 1338, Issue , 2016, Pages 191-206

  Huang, Peng ^a   Xiao, An ^a   Tong, Xiangjun ^a   Lin, Shuo ^a   Zhang, Bo ^a  

^a NONE

Author keywords

Chromosomal deletion; Gene disruption; Gene targeting; Genome manipulation; Homologous recombination; Reverse genetics; TALE nuclease; TALEN; Targeted mutagenesis; Zebrafish

Indexed keywords

GENOMIC DNA; MESSENGER RNA; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNTRANSLATED RNA; RESTRICTION ENDONUCLEASE; ENDONUCLEASE; TRANSACTIVATOR PROTEIN;

CONTROLLED STUDY; DNA TEMPLATE; EMBRYO; GENE CLUSTER; GENE DELETION; GENE DISRUPTION; GENOME; HOMOLOGOUS RECOMBINATION; IN VITRO STUDY; INDEL MUTATION; MICROINJECTION; NONHUMAN; RNA TRANSCRIPTION; SITE DIRECTED MUTAGENESIS; SOUTHERN BLOTTING; ZEBRA FISH; ADULT; ANIMAL EXPERIMENT; CHROMOSOME DELETION; EXPRESSION VECTOR; GENE EDITING; GENE KNOCK-IN; GENE TARGETING; GENETIC MANIPULATION; GENETIC MODIFICATION; HOMOLOGOUS CHROMOSOME; MUTAGENESIS; PROMOTER REGION; REVERSE GENETICS; SURVIVAL RATE; ANIMAL; GENETIC ENGINEERING; GENETICS; GENOMICS; HUMAN; METABOLISM; PROCEDURES; RNA EDITING;

ANIMALS; ENDONUCLEASES; GENE TARGETING; GENETIC ENGINEERING; GENOMICS; HUMANS; RNA EDITING; TRANS-ACTIVATORS; ZEBRAFISH;

EID: 84943558107     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2932-0_15     Document Type: Chapter

References (14)

1. Blackburn PR, Campbell JM, Clark KJ, Ekker SC (2013) The CRISPR system: keeping zebrafish gene targeting fresh. Zebrafish 10(1):116-118
2. Huang P, Zhu Z, Lin S, Zhang B (2012) Reverse genetic approaches in zebrafish. J Genet Genomics 39(9):421-433
3. Wei C, Liu J, Yu Z, Zhang B, Gao G, Jiao R (2013) TALEN or Cas9: rapid, efficient and specific choices for genome modifications. J Genet Genomics 40(6):281-289
4. Xiao A, Wu Y, Yang Z, Hu Y, Wang W, Zhang Y, Kong L, Gao G, Zhu Z, Lin S, Zhang B (2013) EENdb: a database and knowledge base of ZFNs and TALENs for endonuclease engineering. Nucleic Acids Res 41(Database issue):D415-D422
5. Huang P, Xiao A, Zhou M, Zhu Z, Lin S, Zhang B (2011) Heritable gene targeting in zebrafish using customized TALENs. Nat Biotechnol 29(8):699-700
6. Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol 29(8):697-698
7. Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG 2nd, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC (2012) In vivo genome editing using a high-efficiency TALEN system. Nature 491(7422):114-118
8. Zu Y, Tong X, Wang Z, Liu D, Pan R, Li Z, Hu Y, Luo Z, Huang P, Wu Q, Zhu Z, Zhang B, Lin S (2013) TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nat Methods 10(4):329-331
9. Gupta A, Hall VL, Kok FO, Shin M, McNulty JC, Lawson ND, Wolfe SA (2013) Targeted chromosomal deletions and inversions in zebrafish. Genome Res 23(6):1008-1017
10. Lim S, Wang Y, Yu X, Huang Y, Featherstone MS, Sampath K (2013) A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases. Genome Biol 14(7):R69
11. Ma AC, Lee HB, Clark KJ, Ekker SC (2013) High efficiency in vivo genome engineering with a simplified 15-RVD GoldyTALEN design. PLoS One 8(5), e65259
12. Xiao A, Wang Z, Hu Y, Wu Y, Luo Z, Yang Z, Zu Y, Li W, Huang P, Tong X, Zhu Z, Lin S, Zhang B (2013) Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish. Nucleic Acids Res 41(14), e141
13. Meeker ND, Hutchinson SA, Ho L, Trede NS (2007) Method for isolation of PCR-ready genomic DNA from zebrafish tissues. Biotechniques 43(5):610, 612, 614
14. Wang D, Jao LE, Zheng N, Dolan K, Ivey J, Zonies S, Wu X, Wu K, Yang H, Meng Q, Zhu Z, Zhang B, Lin S, Burgess SM (2007) Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions. Proc Natl Acad Sci U S A 104(30):12428-12433