Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris

Journal of Biomolecular NMR

Volumn 62, Issue 3, 2015, Pages 239-245

  Clark, Lindsay ^a   Zahm, Jacob A ^a   Ali, Rustam ^a   Kukula, Maciej ^c   Bian, Liangqiao ^c   Patrie, Steven M ^b   Gardner, Kevin H ^d   Rosen, Michael ^a   Rosenbaum, Daniel M ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c UNIVERSITY OF TEXAS AT ARLINGTON   (United States)

^d Structural Biology Initiative   (United States)

Author keywords

Actin; Deuteration; Eukaryotic expression system; Methyl labeling; Pichia pastoris; TROSY

Indexed keywords

ACTIN; FUNGAL PROTEIN; MALTOSE BINDING PROTEIN; METHYL GROUP; TIOCONAZOLE; CARBON; ISOLEUCINE; RECOMBINANT PROTEIN;

ACTIN FILAMENT; ACTIN POLYMERIZATION; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CARBOXY TERMINAL SEQUENCE; CHEMICAL LABELING; ELECTRON MICROSCOPY; FEASIBILITY STUDY; GENE EXPRESSION SYSTEM; HETERONUCLEAR MULTIPLE QUANTUM COHERENCE; HETERONUCLEAR SINGLE QUANTUM COHERENCE; KOMAGATAELLA PASTORIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; X RAY CRYSTALLOGRAPHY; CHEMISTRY; METABOLISM; NUCLEAR MAGNETIC RESONANCE; PICHIA; PROCEDURES;

ACTINS; CARBON ISOTOPES; ISOLEUCINE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PICHIA; RECOMBINANT PROTEINS;

EID: 84943353072     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-015-9939-2     Document Type: Article

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