Perdeuteration and methyl-selective 1H, 13C-labeling by using a Kluyveromyces lactis expression system

Journal of Biomolecular NMR

Volumn 57, Issue 3, 2013, Pages 297-304

  Miyazawa Onami, Mayumi ^a   Takeuchi, Koh ^b   Takano, Toshiaki ^a   Sugiki, Toshihiko ^a   Shimada, Ichio ^b,c   Takahashi, Hideo ^b,d  

^a Koto ku   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Eukaryotic expression system; Kluyveromyces lactis; Large molecular weight protein; Methyl selective 1H, 13C labeling; Perdeuteration; Stable isotope labeling

Indexed keywords

BACTERIAL PROTEIN; BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE; CARBON 13; METHYL GROUP; PROTON;

ARTICLE; BIOSYNTHESIS; CHEMICAL REACTION; ESCHERICHIA COLI; KLUYVEROMYCES LACTIS; MITOCHONDRIAL MEMBRANE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PERDEUTERATION; PRECURSOR; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ESCHERICHIA COLI; EUKARYOTA; KLUYVEROMYCES LACTIS; PROKARYOTA;

CARBON ISOTOPES; DEUTERIUM; GENE EXPRESSION; ISOLEUCINE; ISOTOPE LABELING; KLUYVEROMYCES; LEUCINE; MALTOSE-BINDING PROTEINS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEINS; TRANSAMINASES; VALINE;

EID: 84890119562     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-013-9789-8     Document Type: Article

References (18)

1. Colón M, Hernández F, López K, Quezada H, González J, López G, Aranda C, González A (2011) Saccharomyces cerevisiae Bat1 and Bat2 aminotransferases have functionally diverged from the ancestral-like Kluyveromyces lactis orthologous enzyme. PLoS One 6:e16099
2. Gardner KH, Zhang X, Gehring K, Kay LE (1998) Solution NMR studies of a 42 KDa Escherichia Coli maltose binding protein/β-cyclodextrin complex: chemical shift assignments and analysis. J Am Chem Soc 120:11738-11748
3. Gossert AD, Hinniger A, Gutmann S, Jahnke W, Strauss A, Fernández C (2011) A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility. J Biomol NMR 51:449-456
4. Gross JD, Gelev VM, Wagner G (2003) A sensitive and robust method for obtaining intermolecular NOEs between side chains in large protein complexes. J Biomol NMR 25:235-242
5. Harju S, Fedosyuk H, Peterson K (2004) Rapid isolation of yeast genomic DNA: bust n' Grab. BMC Biotechnol 4:8
6. Kellis M, Birren BW, Lander ES (2004) Proof and evolutionary analysis of ancient genome duplication in the yeast Saccharomyces cerevisiae. Nature 428:617-624
7. Matsumoto M, Ueda T, Shimada I (2010) Theoretical analyses of the transferred cross-saturation method. J Magn Reson 205:114-124
8. Morgan WD, Kragt A, Feeney J (2000) Expression of deuterium-isotope- labelled protein in the yeast Pichia pastoris for NMR studies. J Biomol NMR 17:337-347
9. Ryan ED, Kohlhaw GB (1974) Subcellular localization of isoleucine-valine biosynthetic enzymes in yeast. J Bacteriol 120:631-637
10. Ryan ED, Tracy JW, Kohlhaw GB (1973) Subcellular localization of the leucine biosynthetic enzymes in yeast. J Bacteriol 116:222-225
11. Sastry M, Xu L, Georgiev IS, Bewley CA, Nabel GJ, Kwong PD (2011) Mammalian production of an isotopically enriched outer domain of the HIV-1 gp120 glycoprotein for NMR spectroscopy. J Biomol NMR 50:197-207
12. Shimada I, Ueda T, Matsumoto M, Sakakura M, Osawa M, Takeuchi K, Nishida N, Takahashi H (2009) Cross-saturation and transferred cross-saturation experiments. Prog Nucl Magn Reson Spectrosc 54:123-140
13. Solà A, Maaheimo H, Ylönen K, Ferrer P, Szyperski T (2004) Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris. Eur J Biochem 271:2462-2470
14. Solà A, Jouhten P, Maaheimo H, Sánchez-Ferrando F, Szyperski T, Ferrer P (2007) Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates. Microbiology 153:281-290
15. Sugiki T, Shimada I, Takahashi H (2008) Stable isotope labeling of protein by Kluyveromyces lactis for NMR study. J Biomol NMR 42:159-162
16. Takahashi H, Nakanishi T, Kami K, Arata Y, Shimada I (2000) A novel NMR method for determining the interfaces of large protein-protein complexes. Nat Struct Biol 7:220-223
17. 13C NMR spectroscopy of methyl groups in very high molecular weight proteins and protein complexes. J Am Chem Soc 125:10420-10428
18. Tugarinov V, Sprangers R, Kay LE (2007) Probing side-chain dynamics in the proteasome by relaxation violated coherence transfer NMR spectroscopy. J Am Chem Soc 129:1743-1750