SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy

Nature Communications

Volumn 6, Issue , 2015, Pages

  Lukinavičius, Gražvydas ^a   Blaukopf, Claudia ^b   Pershagen, Elias ^c   Schena, Alberto ^a   Reymond, Luc ^a   Derivery, Emmanuel ^d   Gonzalez Gaitan, Marcos ^d   D'Este, Elisa ^e   Hell, Stefan W ^e   Gerlich, Daniel Wolfram ^b   Johnsson, Kai ^a  

^a EPFL   (Switzerland)

^b ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^c UPPSALA UNIVERSITY   (Sweden)

^d UNIVERSITY OF GENEVA   (Switzerland)

^e MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BENZIMIDAZOLE DERIVATIVE; DNA; SIR HOECHST; UNCLASSIFIED DRUG; FLUORESCENT DYE;

CELLS AND CELL COMPONENTS; DNA; MICROSCOPY; NANOTECHNOLOGY; PROBE; TOXIC MATERIAL;

ARTICLE; BLUE LIGHT; CELL MEMBRANE PERMEABILITY; CELL PROLIFERATION; CELLS BY BODY ANATOMY; CHEMICAL STRUCTURE; CONTROLLED STUDY; CYTOTOXICITY; EPIFLUORESCENCE MICROSCOPY; FEMALE; HELA CELL LINE; HUMAN; HUMAN CELL; ILLUMINATION; MICROSCOPY; PHOTOTOXICITY; STAIN; STAINING; SYNTHESIS; ANIMAL; CELL LINE; CHEMISTRY; DROSOPHILA; FLUORESCENCE MICROSCOPY; MOLECULAR IMAGING; PROCEDURES;

ANIMALS; CELL LINE; DNA; DROSOPHILA; FLUORESCENT DYES; HUMANS; MICROSCOPY; MICROSCOPY, FLUORESCENCE; MOLECULAR IMAGING; MOLECULAR STRUCTURE; STAINING AND LABELING;

EID: 84943139292     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms9497     Document Type: Article

References (20)

1. Conrad, C. & Gerlich, D. W. Automated microscopy for high-content RNAi screening. J. Cell. Biol. 188, 453-461 (2010).
2. Purschke, M., Rubio, N., Held, K. D. & Redmond, R. W. Phototoxicity of Hoechst 33342 in time-lapse fluorescence microscopy. Photochem. Photobiol. Sci. 9, 1634-1639 (2010).
3. Smith, P. J. et al. Characteristics of a novel deep red/infrared fluorescent cellpermeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy. Cytometry 40, 280-291 (2000).
4. Lukinavicius, G. et al. Fluorogenic probes for live-cell imaging of the cytoskeleton. Nat. Methods 11, 731-733 (2014).
5. Lukinavicius, G. et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nat. Chem. 5, 132-139 (2013).
6. Nakamura, A. et al. Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging. Chem. Commun. 50, 6149-6152 (2014).
7. Hoffman, S. et al. Verapamil inhibits proliferation, migration and protein kinase C activity in human retinal pigment epithelial cells. Exp. Eye Res. 67, 45-52 (1998).
8. Schmidt, W. F., Huber, K. R., Ettinger, R. S. & Neuberg, R. W. Antiproliferative effect of verapamil alone on brain tumor cells in vitro. Cancer Res. 48, 3617-3621 (1988).
9. Giepmans, B. N., Adams, S. R., Ellisman, M. H. & Tsien, R. Y. The fluorescent toolbox for assessing protein location and function. Science 312, 217-224 (2006).
10. Ricci, M. A., Manzo, C., Garcia-Parajo, M. F., Lakadamyali, M. & Cosma, M. P. Chromatin fibers are formed by heterogeneous groups of nucleosomes in vivo. Cell 160, 1145-1158 (2015).
11. Kim, E. et al. Optimized near-IR fluorescent agents for in vivo imaging of Btk expression. Bioconjug. Chem. 6, 1513-1518 (2015).
12. Grimm, J. B. et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12, 244-250. 243 p following 250 (2015)
13. D'Este, E., Kamin, D., Gottfert, F., El-Hady, A. & Hell, S. W. STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons. Cell Rep. 10, 1246-1251 (2015).
14. Wang, B., Chai, X., Zhu, W., Wang, T. & Wu, Q. A general approach to spirolactonized Si-rhodamines. Chem. Commun. 50, 14374-14377 (2014).
15. Kim, E., Yang, K. S., Giedt, R. J. & Weissleder, R. Red Si-rhodamine drug conjugates enable imaging in GFP cells. Chem. Commun. 50, 4504-4507 (2014).
16. Erdmann, R. S. et al. Super-resolution imaging of the Golgi in live cells with a bioorthogonal ceramide probe. Angew. Chem. Int. Ed. 53, 10242-10246 (2014).
17. Breusegem, S. Y., Clegg, R. M. & Loontiens, F. G. Base-sequence specificity of Hoechst 33258 and DAPI binding to five (A/T)4 DNA sites with kinetic evidence for more than one high-affinity Hoechst 33258-AATT complex. J. Mol. Biol. 315, 1049-1061 (2002).
18. Steigemann, P. et al. Aurora B-mediated abscission checkpoint protects against tetraploidization. Cell 136, 473-484 (2009).
19. Loubery, S. & Gonzalez-Gaitan, M. Monitoring notch/delta endosomal trafficking and signaling in Drosophila. Methods Enzymol. 534, 301-321 (2014).
20. Held, M. et al. CellCognition: time-resolved phenotype annotation in highthroughput live cell imaging. Nat. Methods 7, 747-754 (2010).