Active site cleft mutants of Os9BGlu31 transglucosidase modify acceptor substrate specificity and allow production of multiple kaempferol glycosides

Biochimica et Biophysica Acta - General Subjects

Volumn 1850, Issue 7, 2015, Pages 1405-1414

  Komvongsa, Juthamath ^a   Luang, Sukanya ^a   Marques, Joaquim V ^b   Phasai, Kannika ^a   Davin, Laurence B ^b   Lewis, Norman G ^b   Ketudat Cairns, James R ^a,c  

^a SURANAREE UNIVERSITY OF TECHNOLOGY   (Thailand)

^b WASHINGTON STATE UNIVERSITY   (United States)

^c Krung Thep Maha Nakhon   (Thailand)

Author keywords

Electrospray ionization mass spectrometry; Glycoside hydrolase family GH1; Glycosylation; Rice (Oryza sativa L.); Site directed mutagenesis; Transglucosidase

Indexed keywords

4 NITROPHENYL BETA DEXTRO GLUCOPYRANOSIDE; ASTRAGALIN; GLUCOPYRANOSIDE; GLUCOSIDASE; GLYCOSIDE; HYDROXYL GROUP; KAEMPFEROL; KAEMPFEROL 4 O GLUCOSIDE; KAEMPFEROL 7 O GLYCOSIDE; UNCLASSIFIED DRUG; KAEMPFEROL DERIVATIVE; KAEMPFEROL-3-O-GLUCOSIDE; MONOSACCHARIDE; MUTANT PROTEIN; VEGETABLE PROTEIN;

ARTICLE; BINDING SITE; ENZYME ANALYSIS; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GLYCOSYLATION; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; METABOLISM; MUTATION; PROTEIN TERTIARY STRUCTURE; RICE; TANDEM MASS SPECTROMETRY;

CATALYTIC DOMAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLUCOSIDASES; KAEMPFEROLS; KINETICS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MONOSACCHARIDES; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION; ORYZA SATIVA; PLANT PROTEINS; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY; TANDEM MASS SPECTROMETRY;

EID: 84942693641     PISSN: 03044165     EISSN: 18728006     Source Type: Journal    
DOI: 10.1016/j.bbagen.2015.03.013     Document Type: Article

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