Enterovirus71 virus-like particles produced from insect cells and purified by multistep chromatography elicit strong humoral immune responses in mice

Journal of Applied Microbiology

Volumn 119, Issue 4, 2015, Pages 1196-1205

  Zhao, D ^a   Sun, B ^a   Jiang, H ^b   Sun, S ^a   Kong, F T ^c   Ma, Y ^d   Jiang, L ^a   Bai, L ^d   Chen, X ^d   Yang, P ^d   Liu, C ^a   Xu, Y ^a   Su, W ^a,e   Kong, W ^a   Xu, F ^a   Jiang, C ^a  

^a JILIN UNIVERSITY   (China)

^b CHINA JAPAN UNION HOSPITAL OF JILIN UNIVERSITY   (China)

^c Pioneer High School   (United States)

^d Changchun BCHT Biotechnology Co Ltd   (China)

^e CHANGCHUN INSTITUTE OF APPLIED CHEMISTRY   (China)

Author keywords

Chromatography; Enterovirus 71; Immunogenicity; Insect cells; Virus like particle

Indexed keywords

ANTIBODIES; BIOREACTORS; CELLS; CYTOLOGY; DYNAMIC LIGHT SCATTERING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MAMMALS; PURIFICATION; RECOMBINANT PROTEINS; VIRUSES;

CHROMATOGRAPHIC PURIFICATION; ENTEROVIRUS 71; HUMORAL IMMUNE RESPONSE; IMMUNOGENICITY; INSECT CELL SF9; INSECT CELLS; MULTISTEPS; NEUTRALISATION; PURIFICATION PROCESS; VIRUS-LIKE PARTICLES;

HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY;

ENTEROVIRUS 71 VIRUS LIKE PARTICLE VACCINE; NEUTRALIZING ANTIBODY; UNCLASSIFIED DRUG; VIRUS LIKE PARTICLE VACCINE;

BIOREACTOR; CHROMATOGRAPHY; IMMUNE RESPONSE; INDUSTRIAL PRODUCTION; INFECTIVITY; PURIFICATION; RODENT; VACCINE; VIRUS;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY BLOOD LEVEL; ARTICLE; CONTROLLED STUDY; DRUG PURIFICATION; ENTEROVIRUS 71 VIRUS LIKE PARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMORAL IMMUNITY; INSECT CELL; MOLECULAR WEIGHT; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; TRANSMISSION ELECTRON MICROSCOPY; VIRUS LIKE AGENT; VIRUS NEUTRALIZATION; WESTERN BLOTTING; ANIMAL; BAGG ALBINO MOUSE; CELL LINE; CHEMISTRY; CHROMATOGRAPHY; ENTEROVIRUS A; ENTEROVIRUS INFECTION; EVALUATION STUDY; FEMALE; GENETICS; HUMAN; IMMUNIZATION; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PROCEDURES; SPODOPTERA; VIROLOGY;

HEXAPODA; HUMAN ENTEROVIRUS 71; MUS;

ANIMALS; ANTIBODIES, NEUTRALIZING; CELL LINE; CHROMATOGRAPHY; ENTEROVIRUS A, HUMAN; ENTEROVIRUS INFECTIONS; FEMALE; HUMANS; IMMUNITY, HUMORAL; IMMUNIZATION; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; SPODOPTERA;

EID: 84942094155     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12922     Document Type: Article

References (35)

1. Blom, H., Åkerblom, A., Kon, T., Shaker, S., van der Pol, L. and Lundgren, M. (2014) Efficient chromatographic reduction of ovalbumin for egg-based influenza virus purification. Vaccine 372, 1-3724.
2. Champion, K., Madden, H., Dougherty, J. and Shacter, E. (2005) Defining your product profile and maintaining control over it, part 2. Bioprocess Int 3, 52-57.
3. Chen, J., Tetrault, J. and Ley, A. (2008) Comparison of standard and new generation hydrophobic interaction chromatography resins in the monoclonal antibody purification process. J Chromatogr A 1177, 272-281.
4. Chou, A.H., Liu, C.C., Chang, C.P., Guo, M.S., Hsieh, S.Y., Yang, W.H., Chao, H.J., Wu, C.L. et al. (2012) Pilot scale production of highly efficacious and stable enterovirus 71 vaccine candidates. PLoS One 7, e34834.
5. Chung, Y.C., Huang, J.H., Lai, C.W., Sheng, H.C., Shih, S.R., Ho, M.S. and Hu, Y.C. (2006) Expression, purification and characterization of enterovirus-71 virus-like particles. World J Gastroenterol 12, 921-927.
6. Chung, Y.C., Ho, M.S., Wu, J.C., Chen, W.J., Huang, J.H., Chou, S.T. and Hu, Y.C. (2008) Immunization with virus-like particles of enterovirus 71 elicits potent immune responses and protects mice against lethal challenge. Vaccine 26, 1855-1862.
7. FDA (1997) Points to consider in the manufacture and testing of monoclonal antibody products for human use (1997). U.S. Food and Drug Administration Center for Biologics Evaluation and Research. J Immunother (Hagerstown, Md.) 20, 214-243.
8. Ho, M., Chen, E.R., Hsu, K.H., Twu, S.J., Chen, K.T., Tsai, S.F., Wang, J.R. and Shih, S.R. (1999) An epidemic of enterovirus 71 infection in Taiwan. N Engl J Med 341, 929-935.
9. Jin, J., Xu, L., Guo, S.J., Sun, S.Y., Zhang, S., Zhu, C.L., Kong, W. and Jiang, C.L. (2012) Safe and objective assay of enterovirus 71 neutralizing. Chem Res Chinese U 28, 91-95.
10. Ku, Z.Q., Ye, X.H., Huang, X.L., Cai, Y.C., Liu, Q.W., Li, Y., Su, Z.G. and Huang, Z. (2013) Neutralizing antibodies induced by recombinant virus-like particles of enterovirus 71 genotype C4 inhibit infection at pre- and post-attachment steps. PLoS One 8, e57601.
11. Ku, Z.Q., Liu, Q.W., Ye, X.H., Wang, X.L., Shi, J.P., Li, D.P., Jin, X., An, W.Q. et al. (2014) A virus-like particle based bivalent vaccine confers dual protection against enterovirus 71 and coxsackievirus A16 infections in mice. Vaccine 32, 4296-4303.
12. Lee, M.S. and Chang, L.Y. (2010) Development of enterovirus 71 vaccines. Expert Rev Vaccines 9, 149-156.
13. Lee, D.H., Bae, J.E., Lee, J.H., Shin, J.S. and Kim, I.S. (2010) Quantitative detection of residual E. coli host cell DNA by Real-Time PCR. J Microbiol Biotechnol 20, 1463-1470.
14. Li, H.Y., Han, J.F., Qin, C.F. and Chen, R. (2013) Virus-like particles for enterovirus 71 produced from Saccharomyces cerevisiae potently elicits protective immune responses in mice. Vaccine 31, 3281-3287.
15. Liang, Z.L., Mao, Q.Y., Wang, Y.P., Zhu, F.C., Li, J.X., Yao, X., Gao, F., Xu, X. et al. (2013) Progress on the research and development of inactivated EV71 whole-virus vaccines. Hum Vaccin Immunother 9, 1701-1705.
16. Lin, S.Y., Chung, Y.C., Chiu, W.K., Chiang, B.L. and Hu, Y.C. (2014) Evaluation of the stability of enterovirus 71 virus-like particle. J Biosci Bioeng 117, 366-371.
17. Liu, L.D. (2013) A Protected Study of Inactivated EV71 Vaccine (Human Diploid Cell, KMB-17) in Chinese Infants and Children. Chinese Academy of Medical Sciences.
18. Liu, C.C., Guo, M.S., Lin, F.H., Hsiao, K.N., Chang, K.H., Chou, A.H., Wang, Y.C., Chen, Y.C. et al. (2011) Purification and characterization of enterovirus 71 viral particles produced from vero cells grown in a serum free microcarrier bioreactor system. PLoS One 6, e20005.
19. Lua, L.H., Connors, N.K., Sainsbury, F., Chuan, Y.P., Wibowo, N. and Middelberg, A.P. (2014) Bioengineering virus-like particles as vaccines. Biotechnol Bioeng 111, 425-440.
20. McMinn, P.C. (2002) An overview of the evolution of enterovirus 71 and its clinical and public health significance. FEMS Microbiol Rev 26, 91-107.
21. Plummer, E.M. and Manchester, M. (2011) Viral nanoparticles and virus-like particles: platforms for contemporary vaccine design. Wiley Interdiscip Rev Nanomed Nanobiotechnol 3, 174-196.
22. Ramqvist, T., Andreasson, K. and Dalianis, T. (2007) Vaccination, immune and gene therapy based on virus-like particles against viral infections and cancer. Expert Opin Biol Ther 7, 997-1007.
23. Shi, L., Sanyal, G., Ni, A., Luo, Z., Doshna, S., Wang, B., Graham, T.L., Wang, N. et al. (2005) Stabilization of human papillomavirus virus-like particles by non-ionic surfactants. J Pharm Sci 94, 1538-1551.
24. Solomon, T., Lewthwaite, P., Perera, D., Cardosa, M.J., McMinn, P. and Ooi, M.H. (2010) Virology, epidemiology, pathogenesis, and control of enterovirus 71. Lancet Infect Dis 10, 778-790.
25. Sun, S.Y., Jiang, L.P., Liang, Z.L., Mao, Q.Y., Su, W.H., Zhang, H.F., Li, X.J., Jin, J. et al. (2014) Evaluation of monovalent and bivalent vaccines against lethal Enterovirus 71 and Coxsackievirus A16 infection in newborn mice. Hum Vaccin Immunother 10, 2885-2895.
26. Vicente, T., Roldão, A., Peixoto, C., Carrondo, M.J. and Alves, P.M. (2011) Large-scale production and purification of VLP-based vaccines. J Invertebr Pathol 107, S42-S48.
27. Walsh, G. (2002) Proteins Biochemistry and Biotechnology. pp. 205-206. West Sussex, UK: John Wiley & Sons Ltd.
28. World Health Organization (2011) A Guide to Clinical Management and Public Health Response for Hand, Foot and Mouth Disease (HFMD). Geneva: Switzerland.
29. Xu, J., Qian, Y., Wang, S.X., Serrano, J.M.G., Li, W., Huang, Z.H. and Lu, S. (2010) An emerging infectious disease vaccine target in the Far East? Vaccine 28, 3516.
30. Yi, L., Lu, J., Kung, H.F. and He, M.L. (2011) The virology and developments toward control of human enterovirus 71. Crit Rev Microbiol 37, 313-327.
31. Zhang, Y., Zhu, Z., Yang, W., Ren, J., Tan, X., Mao, N.Y., Xu, S.T., Zhu, S.L. et al. (2010) An emerging recombinant human enterovirus 71 responsible for the 2008 outbreak of hand foot and mouth disease in Fuyang city of China. Virol J 7, 94.
32. Zhang, J.H., Dong, M., Jiang, B.F., Dai, X. and Meng, J.H. (2012) Antigenic characteristics of the complete and truncated capsid protein VP1 of enterovirus 71. Virus Res 167, 337-342.
33. Zhang, Y., Tan, X.J., Cui, A.L., Mao, N.Y., Xu, S.T., Zhu, Z., Zhou, J.H. and Shi, J. (2013) Complete genome analysis of the C4 subgenotype strains of enterovirus 71: predominant recombination C4 viruses persistently circulating in China for 14 years. PLoS One 8, e56341.
34. Zhang, H.F., An, D., Liu, W., Mao, Q.Y., Jin, J., Xu, L., Sun, S.Y., Jiang, L.P. et al. (2014) Analysis of cross-reactive neutralizing antibodies in human HFMD serum with an EV71 Pseudovirus-based assay. PLoS One 9, e100545.
35. Zhao, G.F., Dong, X.Y. and Sun, Y. (2009) Ligands for mixed-mode protein chromatography: principles, characteristics and design. J Biotechnol 144, 3-11.