Dynamic assessment of cell viability, proliferation and migration using real time cell analyzer system (RTCA)

Cytotechnology

Volumn 67, Issue 2, 2015, Pages 379-386

  Roshan Moniri, Mani ^a   Young, Ada ^a   Reinheimer, Kelsey ^a   Rayat, Jarrett ^a   Dai, Long Jun ^a   Warnock, Garth L ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Cytotoxicity assay; Migration assay; RTCA; xCELLigence

Indexed keywords

ANALYZER; ARTICLE; BIOLOGICAL MONITORING; CELL ADHESION; CELL ASSAY; CELL INVASION ASSAY; CELL MIGRATION; CELL MIGRATION ASSAY; CELL PROLIFERATION; CELL PROLIFERATION ASSAY; CELL VIABILITY; COCULTURE; CONTROLLED STUDY; CYTOTOXICITY; CYTOTOXICITY ASSAY; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; MICROELECTRODE; PANCREATIC CANCER CELL LINE; REAL TIME CELL ANALYZER SYSTEM;

EID: 84941731107     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-014-9692-5     Document Type: Article

References (18)

1. Boyden S (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med 115:453–466
2. Dai LJ, Moniri MR, Zeng ZR, Zhou JX, Rayat J, Warnock GL (2011) Potential implications of mesenchymal stem cells in cancer therapy. Cancer Lett 305:8–20. doi:10.1016/j.canlet.2011.02.012
3. Duncan AW, Rattis FM, DiMascio LN, Congdon KL, Pazianos G, Zhao C, Yoon K, Cook JM, Willert K, Gaiano N, Reya T (2005) Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance. Nat Immunol 6:314–322. doi:10.1038/ni1164
4. Friedenstein AJ, Piatetzky S II, Petrakova KV (1966) Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 16:381–390
5. Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP (1968) Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation 6:230–247
6. Krishan A (1975) Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 66:188–193
7. Lingen MW (2003) Endothelial cell migration assay. A quantitative assay for prediction of in vivo biology. Methods Mol Med 78:337–347
8. Menon MB, Ronkina N, Schwermann J, Kotlyarov A, Gaestel M (2009) Fluorescence-based quantitative scratch wound healing assay demonstrating the role of MAPKAPK-2/3 in fibroblast migration. Cell Motil Cytoskelet 66:1041–1047
9. Moniri MR, Sun XY, Rayat J, Dai D, Ao Z, He Z, Verchere CB, Dai LJ, Warnock GL (2012) TRAIL-engineered pancreas-derived mesenchymal stem cells: characterization and cytotoxic effects on pancreatic cancer cells. Cancer Gene Ther 19:652–658. doi:10.1038/cgt.2012.46
10. Qiao L, Xu Z, Zhao T, Zhao Z, Shi M, Zhao RC, Ye L, Zhang X (2008) Suppression of tumorigenesis by human mesenchymal stem cells in a hepatoma model. Cell Res 18:500–507. doi:10.1038/cr.2008.40
11. Reya T, Morrison SJ, Clarke MF, Weissman IL (2001) Stem cells, cancer, and cancer stem cells. Nature 414:105–111. doi:10.1038/35102167
12. Sasportas LS, Kasmieh R, Wakimoto H, Hingtgen S, van de Water JA, Mohapatra G, Figueiredo JL, Martuza RL, Weissleder R, Shah K (2009) Assessment of therapeutic efficacy and fate of engineered human mesenchymal stem cells for cancer therapy. Proc Natl Acad Sci USA 106:4822–4827. doi:10.1073/pnas.0806647106
13. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV (1992) Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13:204–208. doi:10.1002/cyto.990130216
14. Shimokawa I, Higami Y, Horiuchi S, Iwasaki M, Ikeda T (1998) Advanced glycosylation end products in adrenal lipofuscin. J Gerontol Ser A Biol Sci Med Sci 53:B49–B51
15. Siegel R, Naishadham D, Jemal A (2012) Cancer statistics, 2012. CA Cancer J Clin 62:10–29. doi:10.3322/caac.20138
16. Sun XY, Nong J, Qin K, Lu H, Moniri MR, Dai LJ, Warnock GL (2011) MSC (TRAIL)-mediated HepG2 cell death in direct and indirect co-cultures. Anticancer Res 31:3705–3712
17. Wang L, Zhu J, Deng C, Xing WL, Cheng J (2008) An automatic and quantitative on-chip cell migration assay using self-assembled monolayers combined with real-time cellular impedance sensing. Lab Chip 8:872–878
18. Yang B, Wu X, Mao Y, Bao W, Gao L, Zhou P, Xie R, Zhou L, Zhu J (2009) Dual-targeted antitumor effects against brainstem glioma by intravenous delivery of tumor necrosis factor-related, apoptosis-inducing, ligand-engineered human mesenchymal stem cells. Neurosurgery 65:610–624. doi:10.1227/01.NEU.0000350227.61132.A7 discussion 624