Fluorescence-based quantitative scratch wound healing assay demonstrating the role of MAPKAPK-2/3 in fibroblast migration

Cell Motility and the Cytoskeleton

Volumn 66, Issue 12, 2009, Pages 1041-1047

  Menon, Manoj B ^a   Ronkina, Natalia ^a   Schwermann, Jessica ^a   Kotlyarov, Alexey ^a   Gaestel, Matthias ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Cell migration; High throughput; Live cell staining; Phosphorylation; Protein kinase; Scanner based quantification

Indexed keywords

1,1' DIOCTADECYL 3,3,3',3' TETRAMETHYLINDOCARBOCYANINE IODIDE; CYTOCHALASIN D; MAP KINASE ACTIVATED PROTEIN KINASE 2; MAP KINASE ACTIVATED PROTEIN KINASE 3; PROTEIN KINASE; TRACER; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL MIGRATION; COMPUTER PROGRAM; DIAGNOSTIC ACCURACY; EMBRYO; ENZYME DEFICIENCY; FIBROBLAST; FLUORESCENCE ANALYSIS; FLUORESCENCE BASED QUANTITATIVE SCRATCH WOUND HEALING ASSAY; FLUOROSCOPY; IMAGE ANALYSIS; IN VITRO STUDY; MONOLAYER CULTURE; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN FUNCTION; QUANTITATIVE ANALYSIS; VALIDATION PROCESS; WOUND CLOSURE; WOUND HEALING;

ANIMALS; BIOLOGICAL ASSAY; CARBOCYANINES; CELL MOVEMENT; CELLS, CULTURED; EMBRYO, MAMMALIAN; FIBROBLASTS; FLUORESCENCE; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; PROTEIN-SERINE-THREONINE KINASES; STAINING AND LABELING; WOUND HEALING;

EID: 73249143312     PISSN: 08861544     EISSN: 10970169     Source Type: Journal    
DOI: 10.1002/cm.20418     Document Type: Article

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