Rapid quantification of Staphylococcus aureus from endotracheal aspirates of ventilated patients: A proof-of-concept study

Diagnostic Microbiology and Infectious Disease

Volumn 83, Issue 2, 2015, Pages 117-120

  Lacroix, Morgane ^a   Barraud, Olivier ^b   Clavel, Marc ^c   Filiputti, Delphine ^a   Prudent, Sandrine ^a   François, Bruno ^c   Ploy, Marie Cécile ^b   Jestin, Marie Astrid ^a   Rodrigue, Marc ^a   Pachot, Alexandre ^a   Yugueros Marcos, Javier ^a   Moucadel, Virginie ^a  

^a BIOMÉRIEUX   (France)

^b UMR 1092   (France)

^c LIMOGES UNIVERSITY HOSPITAL   (France)

Author keywords

Endotracheal aspirate (ETA); Molecular diagnosis; Staphylococcus aureus; Ventilator associated pneumonia (VAP)

Indexed keywords

AMOXICILLIN; CEFTRIAXONE; CLAVULANIC ACID; FOSFOMYCIN; OFLOXACIN; OXACILLIN;

ARTICLE; BACTERIUM DETECTION; CLINICAL PROTOCOL; CLINICAL STUDY; COLONY FORMING UNIT; CONTROLLED STUDY; EARLY DIAGNOSIS; HUMAN; HUMAN TISSUE; INTENSIVE CARE UNIT; LIMIT OF DETECTION; MOLECULAR DIAGNOSIS; NONHUMAN; PRIORITY JOURNAL; PROOF OF CONCEPT STUDY; QUANTITATIVE ANALYSIS; STAPHYLOCOCCUS AUREUS; TRACHEAL ASPIRATION PROCEDURE; VENTILATOR ASSOCIATED PNEUMONIA; ADVERSE EFFECTS; ARTIFICIAL VENTILATION; BACTERIAL LOAD; EVALUATION STUDY; ISOLATION AND PURIFICATION; PNEUMONIA, VENTILATOR-ASSOCIATED; PROCEDURES; PROSPECTIVE STUDY; STAPHYLOCOCCAL INFECTIONS; TIME FACTOR;

BACTERIAL LOAD; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; PNEUMONIA, VENTILATOR-ASSOCIATED; PROSPECTIVE STUDIES; RESPIRATION, ARTIFICIAL; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; TIME FACTORS;

EID: 84941317150     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2015.06.014     Document Type: Article

References (18)

1. Apfalter P., Stoiser B., Barousch W., Nehr M., Kramer L., Burgmann H. Community-acquired bacteria frequently detected by means of quantitative polymerase chain reaction in nosocomial early-onset ventilator-associated pneumonia. Crit Care Med 2005, 33:1492-1498.
2. Barbier F., Andremont A., Wolff M., Bouadma L. Hospital-acquired pneumonia and ventilator-associated pneumonia: recent advances in epidemiology and management. Curr Opin Pulm Med 2013, 19:216-228.
3. Chastre J., Fagon J.Y. Ventilator-associated pneumonia. Am J Respir Crit Care Med 2002, 165:867-903.
4. File T.M. Recommendations for treatment of hospital-acquired and ventilator-associated pneumonia: review of recent international guidelines. Clin Infect Dis 2010, 51(Suppl. 1):S42-S47.
5. Koenig S.M., Truwit J.D. Ventilator-associated pneumonia: diagnosis, treatment, and prevention. Clin Microbiol Rev 2006, 19:637-657.
6. Leone M., Malavieille F., Papazian L., Meyssignac B., Cassir N., Textoris J., et al. Routine use of Staphylococcus aureus rapid diagnostic test in patients with suspected ventilator-associated pneumonia. Crit Care 2013, 17(4):R170. 1-6.
7. Luna C.M., Bledel I., Raimondi A. The role of surveillance cultures in guiding ventilator-associated pneumonia therapy. Curr Opin Infect Dis 2014, 27:184-193.
8. Melsen W.G., Rovers M.M., Groenwold R.H., Bergmans D.C., Camus C., Bauer T.T., et al. Attributable mortality of ventilator-associated pneumonia: a meta-analysis of individual patient data from randomised prevention studies. Lancet Infect Dis 2013, 13:665-671.
9. Michel F., Franceschini B., Berger P., Arnal J.M., Gainnier M., Sainty J.M., et al. Early antibiotic treatment for BAL-confirmed ventilator-associated pneumonia: a role for routine endotracheal aspirate cultures. Chest 2005, 127:589-597.
10. Nair G.B., Niederman M.S. Ventilator-associated pneumonia: present understanding and ongoing debates. Intensive Care Med 2014, 18(5):572. 1-12.
11. Restrepo M.I., Anzueto A., Arroliga A.C., Afessa B., Atkinson M.J., Ho N.J., et al. Economic burden of ventilator-associated pneumonia based on total resource utilization. Infect Control Hosp Epidemiol 2010, 31:509-515.
12. Restrepo M.I., Peterson J., Fernandez J.F., Qin Z., Fisher A.C., Nicholson S.C. Comparison of the bacterial etiology of early-onset and late-onset ventilator-associated pneumonia in subjects enrolled in 2 large clinical studies. Respir Care 2013, 58:1220-1225.
13. Rios-Licea M.M., Bosques F.J., Arroliga A.C., Galindo-Galindo J.O., Garza-Gonzalez E. Quadruplex real-time quantitative PCR assay for the detection of pathogens related to late-onset ventilator-associated pneumonia: a preliminary report. J Microbiol Methods 2010, 81:232-234.
14. Safdar N., Dezfulian C., Collard H.R., Saint S. Clinical and economic consequences of ventilator-associated pneumonia: a systematic review. Crit Care Med 2005, 33:2184-2193.
15. Scholte J.B., van Dessel H.A., Linssen C.F., Bergmans D.C., Savelkoul P.H., Roekaerts P.M., et al. Endotracheal aspirate and bronchoalveolar lavage fluid analysis: interchangeable diagnostic modalities in suspected ventilator-associated pneumonia?. J Clin Microbiol 2014, 52:3597-3604.
16. Schulte B., Eickmeyer H., Heininger A., Juretzek S., Karrasch M., Denis O., et al. Detection of pneumonia associated pathogens using a prototype multiplexed pneumonia test in hospitalized patients with severe pneumonia. PLoS One 2014, 9(11):e110566. 1-7.
17. Sriramulu D.D., Lunsdorf H., Lam J.S., Romling U. Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung. J Med Microbiol 2005, 54:667-676.
18. Torres A., American Thoracic S., Infectious Disease Society of North A The new American Thoracic Society/Infectious Disease Society of North America guidelines for the management of hospital-acquired, ventilator-associated and healthcare-associated pneumonia: a current view and new complementary information. Curr Opin Crit Care 2006, 12:444-445.