Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 36, 2015, Pages 11377-11382

  Tischbirek, Carsten ^a,b,c   Birkner, Antje ^a,b,c   Jia, Hongbo ^a,b,c   Sakmann, Bert ^a   Konnerth, Arthur ^a,b,c  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b Technische Universität München Munich Cluster for Systems Neurology   (Germany)

^c Munich Center for Integrated Protein Science   (Germany)

Author keywords

Calcium imaging; Mouse cortical circuits; Multicolor functional imaging; Neuronal activity

Indexed keywords

CALCIUM ION; FLUORESCENT DYE; 1,2-BIS(2-AMINOPHENOXY)ETHANE-N,N,N',N'-TETRAACETIC ACID; CALCIUM; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; EGTAZIC ACID;

ACTION POTENTIAL; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; BRAIN TISSUE; CELL ACTIVITY; CELL ATTACHED PATCH CLAMP; FEMALE; FLUOROMETRY; IMAGING AND DISPLAY; MALE; MOUSE; NERVE CELL NETWORK; NERVE CELL STIMULATION; NEUROIMAGING; NONHUMAN; PHOTON; PRIORITY JOURNAL; PYRAMIDAL NERVE CELL; SIGNAL NOISE RATIO; TWO PHOTON BRAIN CALCIUM IMAGING; VISUAL CORTEX; ANALOGS AND DERIVATIVES; ANIMAL; BRAIN CORTEX; C57BL MOUSE; CHEMISTRY; CYTOLOGY; DENDRITE; METABOLISM; MULTIPHOTON MICROSCOPY; NERVE CELL; PATCH CLAMP TECHNIQUE; PHYSIOLOGY; PROCEDURES; REPRODUCIBILITY;

ACTION POTENTIALS; ANIMALS; CALCIUM; CEREBRAL CORTEX; DENDRITES; EGTAZIC ACID; FEMALE; FLUORESCENT DYES; FLUOROMETRY; INDICATORS AND REAGENTS; MALE; MICE, INBRED C57BL; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; NEUROIMAGING; NEURONS; PATCH-CLAMP TECHNIQUES; REPRODUCIBILITY OF RESULTS;

EID: 84941255076     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1514209112     Document Type: Article

References (45)

1. Grienberger C, Konnerth A (2012) Imaging calcium in neurons. Neuron 73(5):862-885.
2. Chen TW, et al. (2013) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499(7458):295-300.
3. Inoue M, et al. (2015) Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12(1):64-70.
4. 2+ based on green fluorescent proteins and calmodulin. Nature 388(6645):882-887.
5. 2+ probe composed of a single green fluorescent protein. Nat Biotechnol 19(2):137-141.
6. Tsien RY (1989) Fluorescent probes of cell signaling. Annu Rev Neurosci 12:227-253.
7. Helmchen F, Denk W (2005) Deep tissue two-photon microscopy. Nat Methods 2(12):932-940.
8. Horton NG, et al. (2013) In vivo three-photon microscopy of subcortical structures within an intact mouse brain. Nat Photonics 7 (3).
9. Stosiek C, Garaschuk O, Holthoff K, Konnerth A (2003) In vivo two-photon calcium imaging of neuronal networks. Proc Natl Acad Sci USA 100(12):7319-7324.
10. Kobat D, et al. (2009) Deep tissue multiphoton microscopy using longer wavelength excitation. Opt Express 17(16):13354-13364.
11. Smith AM, Mancini MC, Nie S (2009) Bioimaging: Second window for in vivo imaging. Nat Nanotechnol 4(11):710-711.
12. Xu C, Wise FW (2013) Recent advances in fibre lasers for nonlinear microscopy. Nat Photonics 7(11):875-882.
13. Oheim M, et al. (2014) New red-fluorescent calcium indicators for optogenetics, photoactivation and multi-color imaging. Biochim Biophys Acta 1843(10):2284-2306.
14. Tada M, Takeuchi A, Hashizume M, Kitamura K, Kano M (2014) A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo. Eur J Neurosci 39(11):1720-1728.
15. 2+ signals in layer 4 cortical neurons in vivo. Proc Natl Acad Sci USA 111(25):9277-9282.
16. Tsien RY (1981) A non-disruptive technique for loading calcium buffers and indicators into cells. Nature 290(5806):527-528.
17. Hofer SB, et al. (2011) Differential connectivity and response dynamics of excitatory and inhibitory neurons in visual cortex. Nat Neurosci 14(8):1045-1052.
18. Rochefort NL, et al. (2009) Sparsification of neuronal activity in the visual cortex at eye-opening. Proc Natl Acad Sci USA 106(35):15049-15054.
19. Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F (2004) Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods 1(1):31-37.
20. Kitamura K, Judkewitz B, Kano M, Denk W, Häusser M (2008) Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo. Nat Methods 5(1):61-67.
21. De Kock CP, Bruno RM, Spors H, Sakmann B (2007) Layer- and cell-type-specific suprathreshold stimulus representation in rat primary somatosensory cortex. J Physiol 581(Pt 1):139-154.
22. Niell CM, Stryker MP (2008) Highly selective receptive fields in mouse visual cortex. J Neurosci 28(30):7520-7536.
23. Eberhard M, Erne P (1991) Calcium binding to fluorescent calcium indicators: Calcium green, calcium orange and calcium crimson. Biochem Biophys Res Commun 180(1):209-215.
24. Ranganathan R, Bacskai BJ, Tsien RY, Zuker CS (1994) Cytosolic calcium transients: Spatial localization and role in Drosophila photoreceptor cell function. Neuron 13(4):837-848.
25. 2+ signals. Cell Calcium 28(4):213-223.
26. 2+ imaging. Nat Methods 3(5):377-383.
27. Hyrc KL, et al. (2013) Synthesis and properties of Asante Calcium Red-a novel family of long excitation wavelength calcium indicators. Cell Calcium 54(4):320-333.
28. Egawa T, et al. (2011) Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region. Chem Commun (Camb) 47(14):4162-4164.
29. Egawa T, et al. (2011) Development of a far-red to near-infrared fluorescence probe for calcium ion and its application to multicolor neuronal imaging. J Am Chem Soc 133(36):14157-14159.
30. 2+ imaging. J Am Chem Soc 134(36):14923-14931.
31. Collot M, et al. (2015) CaRuby-Nano: A novel high affinity calcium probe for dual color imaging. eLife 4.
32. Garaschuk O, et al. (2006) Optical monitoring of brain function in vivo: From neurons to networks. Pflugers Arch 453(3):385-396.
33. 2+ imaging in transgenic mice. J Neurosci 27(8):2145-2149.
34. 2+ channel activation in frog olfactory bulb neurones. J Physiol 487(Pt 2):305-317.
35. 2+-measurements with visible wavelength indicators in isolated cardiac myocytes. Cell Calcium 14(5):359-372.
36. Berger T, et al. (2007) Combined voltage and calcium epifluorescence imaging in vitro and in vivo reveals subthreshold and suprathreshold dynamics of mouse barrel cortex. J Neurophysiol 97(5):3751-3762.
37. Ma H, et al. (2014) Wide-field in vivo neocortical calcium dye imaging using a convectionenhanced loading technique combined with simultaneous multiwavelength imaging of voltage-sensitive dyes and hemodynamic signals. Neurophotonics 1(1):015003.
38. Mittmann W, et al. (2011) Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo. Nat Neurosci 14(8):1089-1093.
39. Ji N, Sato TR, Betzig E (2012) Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex. Proc Natl Acad Sci USA 109(1):22-27.
40. Ouzounov DG, et al. (2014) In vivo three-photon calcium imaging of brain activity from layer 6 neurons in mouse brain. OSA Tech Digest STh5C.2.
41. Dunn AK, Wallace VP, Coleno M, Berns MW, Tromberg BJ (2000) Influence of optical properties on two-photon fluorescence imaging in turbid samples. Appl Opt 39(7):1194-1201.
42. Zhao Q, Guo H, Yi G, Liao J, Diwu Z (2015) A novel red fluorescence calcium indicator for functional analysis of GPCRs and calcium channel targets. Biophys J 108(2):109a-110a.
43. Garaschuk O, Milos RI, Konnerth A (2006) Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo. Nat Protoc 1(1):380-386.
44. Sasaki T, Matsuki N, Ikegaya Y (2012) Targeted axon-attached recording with fluorescent patch-clamp pipettes in brain slices. Nat Protoc 7(6):1228-1234.
45. Jia H, Rochefort NL, Chen X, Konnerth A (2010) Dendritic organization of sensory input to cortical neurons in vivo. Nature 464(7293):1307-1312.