Targeted axon-attached recording with fluorescent patch-clamp pipettes in brain slices

Nature Protocols

Volumn 7, Issue 6, 2012, Pages 1228-1234

  Sasakit, Takuya ^a   Matsuki, Norio ^a   Ikegaya, Yuji ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

ACTION POTENTIAL; ANIMAL; ARTICLE; BRAIN; COMPUTER PROGRAM; CONFOCAL MICROSCOPY; IMAGE PROCESSING; IN VITRO STUDY; INSTITUTE FOR CANCER RESEARCH MOUSE; INSTRUMENTATION; METABOLISM; METHODOLOGY; MOUSE; NERVE FIBER; PATCH CLAMP; PHYSIOLOGY; RAT; WISTAR RAT;

ACTION POTENTIALS; ANIMALS; AXONS; BRAIN; FLUORESCENT DYES; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MICE, INBRED ICR; MICROSCOPY, CONFOCAL; PATCH-CLAMP TECHNIQUES; RATS; RATS, WISTAR; SOFTWARE;

EID: 84865821909     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2012.061     Document Type: Article

References (30)

1. Debanne, D., Information processing in the axon. Nat. Rev. Neurosci. 5, 304-316 (2004).
2. Astman, N., Gutnick, M. J., Fleidervish, I. A., Persistent sodium current in layer 5 neocortical neurons is primarily generated in the proximal axon. J. Neurosci. 26, 3465-3473 (2006).
3. De Polavieja, G. G., Harsch, A., Kleppe, I., Robinson, H. P., Juusola, M., Stimulus history reliably shapes action potential waveforms of cortical neurons. J. Neurosci. 25, 5657-5665 (2005).
4. Shu, Y., Hasenstaub, A., Duque, A., Yu, Y., McCormick, D. A., Modulation of intracortical synaptic potentials by presynaptic somatic membrane potential. Nature 441, 761-765 (2006).
5. Kole, M. H., Letzkus, J. J., Stuart, G. J., Axon initial segment Kv1 channels control axonal action potential waveform and synaptic efficacy. Neuron 55, 633-647 (2007).
6. Shu, Y., Yu, Y., Yang, J., McCormick, D. A., Selective control of cortical axonal spikes by a slowly inactivating K+ current. Proc. Natl. Acad. Sci. USA 104, 11453- 11458 (2007).
7. Alle, H., Geiger, J. R., Combined analog and action potential coding in hippocampal mossy fibers. Science 311, 1290-1293 (2006).
8. Scott, R., Ruiz, A., Henneberger, C., Kullmann, D. M., Rusakov, D. A., Analog modulation of mossy fiber transmission is uncoupled from changes in presynaptic Ca2+. J. Neurosci. 28, 7765-7773 (2008).
9. Christie, J. M., Chiu, D. N., Jahr, C. E. Ca2+-dependent enhancement of release by subthreshold somatic depolarization. Nat. Neurosci. 14, 62-68 (2011).
10. Sasaki, T., Matsuki, N., Ikegaya, Y., Effects of axonal topology on the somatic modulation of synaptic outputs. J. Neurosci. 32, 2868-2876 (2012).
11. Sasaki, T., Matsuki, N., Ikegaya, Y., Action-potential modulation during axonal conduction. Science 331, 599-601 (2011).
12. Awatramani, G. B., Price, G. D., Trussell, L. O., Modulation of transmitter release by presynaptic resting potential and background calcium levels. Neuron 48, 109-121 (2005).
13. Hori, T., Takahashi, T., Mechanisms underlying short-term modulation of transmitter release by presynaptic depolarization. J. Physiol. 587, 2987-3000 (2009).
14. Alle, H., Geiger, J. R., Analog signalling in mammalian cortical axons. Curr. Opin. Neurobiol. 18, 314-320 (2008).
15. Geiger, J. R., Jonas, P., Dynamic control of presynaptic Ca2+ inflow by fast-inactivating K+ channels in hippocampal mossy fiber boutons. Neuron 28, 927-939 (2000).
16. Bischofberger, J., Engel, D., Li, L., Geiger, J. R., Jonas, P., Patch-clamp recording from mossy fiber terminals in hippocampal slices. Nat. Protoc. 1, 2075-2081 (2006).
17. Forsythe, I. D., Direct patch recording from identified presynaptic terminals mediating glutamatergic EPSCs in the rat CNS, in vitro. J. Physiol. 479 (Part 3), 381-387 (1994).
18. Palmer, L. M., et al. Initiation of simple and complex spikes in cerebellar Purkinje cells. J. Physiol. 588, 1709-1717 (2010).
19. Rudolph, S., Overstreet-Wadiche, L., Wadiche J. I., Desynchronization of multivesicular release enhances Purkinje cell output. Neuron 70, 991-1004 (2011).
20. Atherton, J. F., Wokosin, D. L., Ramanathan, S., Bevan, M. D., Autonomous initiation and propagation of action potentials in neurons of the subthalamic nucleus. J. Physiol. 586, 5679-5700 (2008).
21. Monsivais, P., Clark, B. A., Roth, A., Hausser, M., Determinants of action potential propagation in cerebellar Purkinje cell axons. J. Neurosci. 25, 464-472 (2005).
22. Ishikawa, D., et al. Fluorescent pipettes for optically targeted patch-clamp recordings. Neural Netw. 23, 669-672 (2010).
23. Sasaki, T., Takahashi, N., Matsuki, N., Ikegaya, Y., Fast and accurate detection of action potentials from somatic calcium fluctuations. J. Neurophysiol. 100, 1668-1676 (2008).
24. Takahashi, N., Sasaki, T., Matsumoto, W., Matsuki, N., Ikegaya, Y., Circuit topology for synchronizing neurons in spontaneously active networks. Proc. Natl. Acad. Sci. USA 107, 10244-10249 (2010).
25. Koyama, R., et al. A low-cost method for brain slice cultures. J. Pharmacol. Sci. 104, 191-194 (2007).
26. De Simoni, A., Yu, L. M., Preparation of organotypic hippocampal slice cultures: interface method. Nat. Protoc. 1, 1439-1445 (2006).
27. Debanne, D., et al. Paired-recordings from synaptically coupled cortical and hippocampal neurons in acute and cultured brain slices. Nat. Protoc. 3, 1559-1568 (2008).
28. Takahashi, N., et al. High-speed multineuron calcium imaging using Nipkow-type confocal microscopy. Curr. Protoc. Neurosci. 57, 2.14.1-2.14.10 (2011).
29. Perkins, K. L., Cell-attached voltage-clamp and current-clamp recording and stimulation techniques in brain slices. J., Neurosci. Methods 154, 1-18 (2006).
30. Takahashi, N., et al. Locally synchronized synaptic inputs. Science 335, 353-356 2012