DSDecode: A web-based tool for decoding of sequencing chromatograms for genotyping of targeted mutations

Molecular Plant

Volumn 8, Issue 9, 2015, Pages 1431-1433

  Liu, Weizhi ^a,b,c   Xie, Xianrong ^a,b,c   Ma, Xingliang ^a,b,c   Li, Jun ^d   Chen, Jiehu ^d   Liu, Yao Guang ^a,b,c  

^a State Key Laboratory for Conservation and Utilization of Subtropical Agro bioresources   (China)

^b Key Laboratory of Plant Functional Genomics and Biotechnology of Education Department   (China)

^c SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^d Science Corporation of Gene Ltd   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; BIOLOGY; COMPUTER PROGRAM; DNA SEQUENCE; GENETICS; GENOTYPE; INTERNET; MUTATION; PROCEDURES;

ALLELES; COMPUTATIONAL BIOLOGY; GENOTYPE; INTERNET; MUTATION; SEQUENCE ANALYSIS, DNA; SOFTWARE;

EID: 84941188088     PISSN: 16742052     EISSN: 17529867     Source Type: Journal    
DOI: 10.1016/j.molp.2015.05.009     Document Type: Letter

References (8)

1. L. Cong, F.A. Ran, D. Cox, S. Lin, R. Barretto, N. Habib, P.D. Hsu, X. Wu, W. Jiang, L.A. Marraffini, and et al. Multiplex genome engineering using CRISPR/Cas systems Science 339 2013 819 823
2. Z. Feng, Y. Mao, N. Xu, B. Zhang, P. Wei, D.L. Yang, Z. Wang, Z. Zhang, R. Zheng, L. Yang, and et al. Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis Proc. Natl. Acad. Sci. USA 111 2014 4632 4637
3. T. Li, B. Liu, M.H. Spalding, D.P. Weeks, and B. Yang High-efficiency TALEN-based gene editing produces disease-resistant rice Nat. Biotechnol. 30 2012 390 392
4. J.F. Li, J.E. Norville, J. Aach, M. McCormack, D. Zhang, J. Bush, G.M. Church, and J. Sheen Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 Nat. Biotechnol. 31 2013 688 691
5. X. Ma, L. Chen, Q. Zhu, and Y. Liu Rapid decoding of sequence-specific nuclease-induced heterozygous and biallelic mutations by direct sequencing of PCR products Mol. Plant 2015 10.1016/j.molp.2015.02.012
6. X. Ma, Q. Zhang, Q. Zhu, W. Liu, Y. Chen, R. Qiu, B. Wang, Z. Yang, H. Li, Y. Lin, and et al. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants Mol. Plant 2015 10.1016/j.molp.2015.04.007
7. H. Zhang, J. Zhang, P. Wei, B. Zhang, F. Gou, Z. Feng, Y. Mao, L. Yang, H. Zhang, N. Xu, and et al. The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation Plant Biotechnol. J. 12 2014 797 807
8. H. Zhang, F. Gou, J. Zhang, W. Liu, Q. Li, Y. Mao, J.R. Botella, and J.-K. Zhu TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice Plant Biotechnol. J. 2015 10.1111/pbi.12372