Silica bioreplication preserves three-dimensional spheroid structures of human pluripotent stem cells and HepG2 cells

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Lou, Yan Ru ^a   Kanninen, Liisa ^a   Kaehr, Bryan ^b,c   Townson, Jason L ^c,d   Niklander, Johanna ^a   Harjumäki, Riina ^a   Jeffrey Brinker, C ^b,c   Yliperttula, Marjo ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b ADVANCED MATERIALS LABORATORY   (United States)

^c UNIVERSITY OF NEW MEXICO   (United States)

^d UNIVERSITY OF NEW MEXICO SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGEL; SILICON DIOXIDE;

CELL CULTURE TECHNIQUE; CHEMISTRY; CYTOLOGY; FLUORESCENCE MICROSCOPY; HEP-G2 CELL LINE; HUMAN; HYDROGEL; IMMUNOHISTOCHEMISTRY; MULTICELLULAR SPHEROID; PHENOTYPE; PLURIPOTENT STEM CELL; SCANNING ELECTRON MICROSCOPY;

CELL CULTURE TECHNIQUES; HEP G2 CELLS; HUMANS; HYDROGELS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; PHENOTYPE; PLURIPOTENT STEM CELLS; SILICON DIOXIDE; SPHEROIDS, CELLULAR;

EID: 84940661337     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep13635     Document Type: Article

References (39)

1. Thomson, J. A. et al. Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147 (1998).
2. Martin, M. J., Muotri, A., Gage, F., Varki, A. Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat. Med. 11, 228-232 (2005).
3. Brafman, D. A. et al. Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces. Biomaterials 31, 9135-9144 (2010).
4. Irwin, E. F., Gupta, R., Dashti, D. C., Healy, K. E. Engineered polymer-media interfaces for the long-term self-renewal of human embryonic stem cells. Biomaterials 32, 6912-6919 (2011).
5. Villa-Diaz, L. G. et al. Synthetic polymer coatings for long-term growth of human embryonic stem cells. Nat. Biotechnol. 28, 581-583 (2010).
6. Derda, R. et al. High-throughput discovery of synthetic surfaces that support proliferation of pluripotent cells. J. Am. Chem. Soc. 132, 1289-1295 (2010).
7. Rodin, S. et al. Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511. Nat. Biotechnol. 28, 611-615 (2010).
8. Melkoumian, Z. et al. Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells. Nat. Biotechnol. 28, 606-610 (2010).
9. Braam, S. R. et al. Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell selfrenewal via alphavbeta5 integrin. Stem Cells 26, 2257-2265 (2008).
10. Gerecht, S. et al. Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells. Proc. Natl. Acad. Sci. USA 104, 11298-11303 (2007).
11. Li, Z., Leung, M., Hopper, R., Ellenbogen, R., Zhang, M. Feeder-free self-renewal of human embryonic stem cells in 3D porous natural polymer scaffolds. Biomaterials 31, 404-412 (2010).
12. Lu, H. F. et al. A 3D microfibrous scaffold for long-term human pluripotent stem cell self-renewal under chemically defined conditions. Biomaterials 33, 2419-2430 (2012).
13. Siti-Ismail, N., Bishop, A. E., Polak, J. M., Mantalaris, A. The benefit of human embryonic stem cell encapsulation for prolonged feeder-free maintenance. Biomaterials 29, 3946-3952 (2008).
14. Lou, Y. R. et al. The use of nanofibrillar cellulose hydrogel as a flexible three-dimensional model to culture human pluripotent stem cells. Stem Cells Dev. 23, 380-392 (2014).
15. Marinho, P. A. et al. Xeno-free production of human embryonic stem cells in stirred microcarrier systems using a novel animal/human-component-free medium. Tissue Eng. Part C Methods 19, 146-155 (2013).
16. Beier, A. F. et al. Effective surface-based cryopreservation of human embryonic stem cells by vitrification. Cryobiology 63, 175-185 (2011).
17. Kaehr, B. et al. Cellular complexity captured in durable silica biocomposites. Proc. Natl. Acad. Sci. USA 109, 17336-17341 (2012).
18. Townson, J. L. et al. Synthetic fossilization of soft biological tissues and their shape-preserving transformation into silica or electron-conductive replicas. Nat. Commun. 5, 5665 (2014).
19. Cha, J. N., Stucky, G. D., Morse, D. E., Deming, T. J. Biomimetic synthesis of ordered silica structures mediated by block copolypeptides. Nature 403, 289-292 (2000).
20. Coffman, E. A., Melechko, A. V., Allison, D. P., Simpson, M. L., Doktycz, M. J. Surface patterning of silica nanostructures using bio-inspired templates and directed synthesis. Langmuir. 20, 8431-8436 (2004).
21. Pouget, E. et al. Hierarchical architectures by synergy between dynamical template self-assembly and biomineralization. Nat. Mater. 6, 434-439 (2007).
22. Khripin, C. Y., Pristinski, D., Dunphy, D. R., Brinker, C. J., Kaehr, B. Protein-directed assembly of arbitrary three-dimensional nanoporous silica architectures. ACS Nano 5, 1401-1409 (2011).
23. Baca, H. K. et al. Cell-directed assembly of lipid-silica nanostructures providing extended cell viability. Science 313, 337-341 (2006).
24. Baca, H. K. et al. Cell-directed assembly of bio/nano interfaces-a new scheme for cell immobilization. Acc. Chem. Res. 40, 836-845 (2007).
25. Carnes, E. C. et al. Cell-directed localization and orientation of a functional foreign transmembrane protein within a silica nanostructure. J. Am. Chem. Soc. 131, 14255-14257 (2009).
26. Harper, J. C. et al. Cell-directed integration into three-dimensional lipid-silica nanostructured matrices. ACS Nano 4, 5539-5550 (2010).
27. Meyer, K. C., Coker, E. N., Bolintineanu, D. S., Kaehr, B. Mechanically encoded cellular shapes for synthesis of anisotropic mesoporous particles. J. Am. Chem. Soc. 136, 13138-13141 (2014).
28. Bhattacharya, M. et al. Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture. J. Control. Release 164, 291-298 (2012).
29. Malinen, M. M. et al. Differentiation of liver progenitor cell line to functional organotypic cultures in 3D nanofibrillar cellulose and hyaluronan-gelatin hydrogels. Biomaterials 35, 5110-5121 (2014).
30. Lee, J., Cuddihy, M. J., Cater, G. M., Kotov, N. A. Engineering liver tissue spheroids with inverted colloidal crystal scaffolds. Biomaterials 30, 4687-4694 (2009).
31. Li, X. et al. Micro-scaffold array chip for upgrading cell-based high-throughput drug testing to 3D using benchtop equipment. Lab Chip 14, 471-481 (2014).
32. Amit, M. et al. Suspension culture of undifferentiated human embryonic and induced pluripotent stem cells. Stem Cell Rev. 6, 248-259 (2010).
33. Steiner, D. et al. Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat. Biotechnol. 28, 361-364 (2010).
34. Kelm, J. M., Timmins, N. E., Brown, C. J., Fussenegger, M., Nielsen, L. K. Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnol. Bioeng. 83, 173-180 (2003).
35. Subramanian, K. et al. Spheroid culture for enhanced differentiation of human embryonic stem cells to hepatocyte-like cells. Stem Cells Dev. 23, 124-131 (2014).
36. Braet, F., De Zanger, R., Wisse, E. Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells. J. Microsc. 186, 84-87 (1997).
37. Niu, L. N. et al. Biomimetic silicification of demineralized hierarchical collagenous tissues. Biomacromolecules 14, 1661-1668 (2013).
38. Ying, J. Y. Biocomposites. Cells made of silica. Nat. Nanotechnol. 7, 777-778 (2012).
39. Yu, J. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920 (2007).